biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:783-787, 2014 | DOI: 10.1007/s10535-014-0448-z

Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

N. Cerovska1, H. Plchova1,*, P. Vaculik1,2, T. Moravec1, T. Gichner1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
2 Faculty of Science, Charles University in Prague, Prague 2, Czech Republic

We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail moment value, was observed in the inoculated leaves and decreased in the 1st to 4th systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rate of somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi.

Keywords: Comet assay; ethyl methanesulphonate; single cell gel electrophoresis; somatic mutations
Subjects: DNA damage; leaf nuclei; comet assay; ethyl methanesulphonate; mutant; virus infection; tobacco

Received: March 6, 2014; Revised: June 4, 2014; Accepted: June 11, 2014; Published: December 1, 2014  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cerovska, N., Plchova, H., Vaculik, P., Moravec, T., & Gichner, T. (2014). Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi. Biologia plantarum, 58(4), 783-787. doi: 10.1007/s10535-014-0448-z
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download filebpl-201404-0023_S1.pdf

    File size: 174.88 kB

    References

    1. Anindya, R., Savithri, H.S.: Potyviral NIa proteinase, a proteinase with novel deoxyribonuclease activity. - J. biol. Chem. 279; 32159-32169, 2004. Go to original source...
    2. Atabekov, J., Dobrov, E., Rodionova, N.: Potato virus X; structure, disassembly and reconstitution. - Mol. Plant Pathol. 8; 667-675, 2007. Go to original source...
    3. Batten, J.S., Yoshinari, S., Hemengway, C.: Potato virus X; a model system for virus replication, movement and gene expression. - Mol. Plant Pathol. 4; 125-131, 2003. Go to original source...
    4. Boyko, A., Kovalchuk, I.: Genetic and epigenetic effects of plant-pathogen interactions: an evolutionary perspective. - Mol. Plant 4; 1014-1023, 2011. Go to original source...
    5. Carrington, J.C., Freed, D.D., Leinicke, A.J.: Bipartite signal sequence mediates nuclear translocation of the plant potyviral NIa protein. - Plant Cell 3; 953-962, 1991. Go to original source...
    6. Cerovska, N., Hoffmeisterova, H., Pecenkova, T., Moravec, T., Synkova, H., Plchova, H., Veleminsky, J.: Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector. - Protein exp. Purification 58; 154-161, 2008. Go to original source...
    7. Cvjetko, P., Balen, B., Peharec ©tefanić, P., DebogoviĆ, L., Pavlica, M., Klobučar, G.I.V.: Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants. - Biol. Plant. 58; 71-79, 2014. Go to original source...
    8. Dulieu, H.L., Dalebroux, M.A.: Spontaneous and induced reversion rates in a double heterozygous mutant of Nicotiana tabacum var. xanthi n.c. - dose-response relationship. - Mutat. Res. 30; 63-69, 1975. Go to original source...
    9. Dulieu, H.L.: Somatic variations on a yellow mutant in Nicotiana tabacum (a 1 +/a 1; a 2 + +/a 2) II. Reciprocal genetic events occurring in leaf cells. - Mutat. Res. 28; 69-77, 1975. Go to original source...
    10. Gao, R., Liu, P., Wong S.M.: Identification of a plant viral RNA genome in the nucleus. - PLoS ONE 7; e48736, 2012. Go to original source...
    11. Gichner, T.: DNA damage induced by indirect and direct acting mutagens in catalase-deficient transgenic tobacco. Cellular and acellular Comet assays. - Mutat. Res. 535; 187-193, 2003. Go to original source...
    12. Gichner, T., Plewa, M.J.: Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. - Mutat. Res. 401; 143-152, 1998. Go to original source...
    13. Gichner, T., Ptacek, O., Stavreva D.A., Plewa, M.J.: Comparison of DNA damage in plants as measured by single cell gel electrophoresis and somatic leaf mutations induced by monofunctional alkylating agents. - Environ. mol. Mutagen. 35; 279-286, 1999. Go to original source...
    14. Gichner, T., Znidar, I., Wagner, E.D., Plewa, M.J.: The use of higher plants in the comet assay. - In Dhawan, A., Anderson, D. (ed.): The Comet Assay in Toxicology. Pp. 98-119. Royal Society of Chemistry, Cambridge 2009. Go to original source...
    15. Huisman, M.J., Linthorst, H.J.M., Bol, J.F., Cornelissen, B.J.C.: The complete nucleotide sequence of Potato virus X and its homologies at the amino acid level with various plusstranded RNA viruses. - J. gen. Virol. 69; 1789-1798, 1988. Go to original source...
    16. Ju, H.J., Ye, C.M., Verchot-Lubicz, J.: Mutational analysis of PVX TGBp3 links subcellular accumulation and protein turnover. - Virology 375; 103-117, 2008. Go to original source...
    17. Koppen, G., Verschaeve, L.: The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells. - Mutat. Res. 360; 193-200, 1996. Go to original source...
    18. Kovalchuk, I., Kovalchuk, O., Kalck, V., Boyko, V., Filkowski, J., Heinlein, M., Hohn, B.: Pathogen-induced systemic plant signal triggers DNA rearrangements. - Nature 423; 760-762, 2003. Go to original source...
    19. Krichevsky, A., Kozlovsky, S.V., Gafni, Y., Citovsky, V.: Nuclear import and export of plant virus proteins and genomes. - Mol. Plant Pathol. 7; 131-146, 2006. Go to original source...
    20. Li, X.H., Carrington, J.C.: Nuclear transport of tobacco etch potyviral RNA-dependent RNA polymerase is highly sensitive to sequence alterations. - Virology 193; 951-958, 1993. Go to original source...
    21. Lucht, J.M., Mauch-Mani, B., Steiner, H.Y., Metraux, J.P., Ryals, J., Hohn, B.: Pathogen stress increases somatic recombination frequency in Arabidopsis. - Natur. Genet. 30; 311-314, 2002. Go to original source...
    22. Mehdy, M.C.: Active oxygen species in plant defense against pathogens. - Plant Physiol. 105; 467-472, 1994. Go to original source...
    23. Procházková, D., Wilhelmová, N., PavlÍková, D., Száková, J., Gichner, T.: Zinc induces DNA damage in tobacco roots. - Biol. Plant. 57; 783-787, 2013. Go to original source...
    24. Ptacek, O., Stavreva D.A., Kim, J.K., Gichner, T.: Induction and repair of DNA damage as measured by the Comet assay and the yield of somatic mutations in gamma-irradiated tobacco seedlings. - Mutat. Res. 491:17-23, 2001. Go to original source...
    25. Ritzenthaler, C.: Parallels and distinctions in the direct cell-tocell spread of the plant and animal viruses. - Curr. Opin.Virol. 1; 403-409, 2011. Go to original source...
    26. Ryabov, E.V., Kim, S.H., Taliansky, M.: Identification of a nuclear localization signal and nuclear export signal of the umbraviral long-distance RNA movement protein. - J. gen. Virol. 85; 1329-1333, 2004. Go to original source...
    27. Skryabin, K.G., Kraev, A.S., Morozov, S.Y., Rozanov, M.N., Chernov, B.K., Lukasheva, L.I., Atabekov, J.G.: The nucleotide sequence of Potato virus X RNA. - Nucl. Acids Res. 16; 10929-10930, 1988. Go to original source...
    28. Sonenberg, N., Shatkin, A.J., Riccardi, R.P., Rubin, M., Goodman, R.M.: Analysis of terminal structures of RNA from potato virus X. - Nucl. Acids Res. 5; 2501-2521, 1978. Go to original source...
    29. Stavreva, D.A., Gichner, T.: DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage. - Mutat. Res. 514; 147-152, 2002. Go to original source...
    30. Termini, J.: Hydroperoxide-induced DNA damage and mutations. - Mutat. Res. 450; 107-124, 2000. Go to original source...
    31. Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F.: Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. - Environ. mol. Mutagen. 35; 206-221, 2000. Go to original source...
    32. Turnell, A.S., Grand, R.J.: DNA viruses and the cellular DNA-damage response. - J. gen. Virol. 93; 2076-2097, 2012. Go to original source...
    33. Verchot-Lubicz, J.: A new cell-to-cell transport model for potexviruses. - Mol. Plant-Microbe Interact. 18; 283-290, 2005. Go to original source...
    34. Verchot-Lubicz, J., Ye, C.M., Bamunusinghe, J.: Molecular biology of potexviruses: recent advances. - J. gen. Virol. 88; 1643-1655, 2007. Go to original source...
    35. Vetter, G., Hily, J.M., Klein, E., Schmidlin, L., Haas, M., Merkle, T., Gilmer, D.: Nucleo-cytoplasmic shuttling of the beet necrotic yellow vein virus RNA-3-encoded p25 protein. - J. gen. Virol. 85; 2459-2469, 2004. Go to original source...
    36. Weitzman, M.D., Lilley, C.E., Chaurushiya, M.S.: Genomes in conflict: maintaining genome integrity during virus infection. - Annu. Rev. Microbiol. 64; 61-81, 2010. Go to original source...
    37. Yao, Y., Bilichak, A., Golubov, A., Kovalchuk, I.: Local infection with oilseed rape mosaic virus promotes genetic rearrangements in systemic Arabidopsis tissue. - Mutat. Res. 709-710; 7-14, 2011. Go to original source...

    Return to the content