biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 59:187-192, 2015 | DOI: 10.1007/s10535-014-0481-y

Rice leaf heterogeneity in chlorophyll fluorescence parameters under short-term osmotic stress

J. Z. Li1, Y. P. Chen1, K. Q. Teng1, L. Z. Qin1, Y. X. Du1, J. Zhang1, Q. Z. Zhao1,*
1 Collaborative Innovation Center of Henan Grain Crops, Rice Engineer Center and Key Laboratory of Physiology, Ecology and Genetics Improvement of Food Crop in Henan Province, Henan Agricultural University, Zhengzhou, P.R. China

The effects of short-term osmotic stress [a polyethylene glycol (PEG) treatment] on photosystem II (PS II) of upland and lowland rice seedlings were investigated using chlorophyll (Chl) fluorescence imaging. Spatial heterogeneity in the top fully expanded leaf for all Chl fluorescence parameters was found under stress conditions. After exposure to PEG, a decrease in the effective quantum yield of PS II photochemistry (φPS II) and photochemical quenching (qP), and an increase in non-photochemical quenching (NPQ) proceeded from the upper section to the base of the leaf. The most sensitive position in the leaf was different between the two ecotypes. Chl fluorescence parameters, net photosynthetic rates (PN), and stomatal conductance (gs) were more sensitive to the PEG stress in upland rice than in lowland rice. These results also indicate different leaf anatomy and development in the two rice ecotypes. Additionally, the findings suggest a more rapid stress response in upland rice.

Keywords: chlorophyll fluorescence imaging; net photosynthetic rate; Oryza sativa; polyethylene glycol; stomatal conductance
Subjects: leaf heterogeneity; chlorophyll fluorescence; osmotic stress; chlorophyll fluorescence imaging; net photosynthetic rate; stomatal conductance; rice

Received: March 8, 2014; Revised: July 17, 2014; Accepted: July 28, 2014; Published: January 1, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Li, J.Z., Chen, Y.P., Teng, K.Q., Qin, L.Z., Du, Y.X., Zhang, J., & Zhao, Q.Z. (2015). Rice leaf heterogeneity in chlorophyll fluorescence parameters under short-term osmotic stress. Biologia plantarum, 59(1), 187-192. doi: 10.1007/s10535-014-0481-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download filebpl-201501-0023_S1.pdf

    File size: 207.68 kB

    References

    1. Ainsworth, E.A., Walter, A., Schurr, U.: Glycine max leaflets lack a base-tip gradient in growth rate. - J. Plant. Res. 118: 343-346, 2005. Go to original source...
    2. Calatayud, A., Roca, D., Martínez, P.F.: Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging. - Plant. Physiol. Biochem. 44: 564-573, 2006. Go to original source...
    3. Gao, F.H., Zhang, H.L., Wang, H.G., Gao, H., Li, Z.C.: Comparative transcriptional profiling under drought stress between upland and lowland rice (Oryza sativa L.) using cDNA-AFLP. - Chin. sci. Bull. 54: 3555-3571, 2009. Go to original source...
    4. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    5. Giardi, M.T., Cona, A., Geiken, B., Kučera, T., Masojidek, J., Mattoo, A.K.: Long-term drought stress induces structural and functional reorganization of photosystem II. - Planta 199: 118-125, 1996. Go to original source...
    6. Hu, S.P., Zhou, Y., Zhang, L., Zhu, X.D., Li, L., Luo, L.J., Liu, G.L., Zhou, Q.M.: Correlation and quantitative trait loci analyses of total chlorophyll content and photosynthetic rate of rice (Oryza sativa) under water stress and well-watered conditions. - J. integr. Plant Biol. 51: 879-888, 2009. Go to original source...
    7. Iqbal, M.J., Goodwin, P.H., Leonardos, E.D., Grodzinski, B.: Spatial and temporal changes in chlorophyll fluorescence images of Nicotiana benthamiana leaves following inoculation with Pseudomonas syringae pv. tabaci. - Plant. Pathol. 61: 1052-1062, 2012. Go to original source...
    8. Li, S., Zhang, Y.J., Sack, L., Scoffoni, C., Ishida, A., Chen, Y.J., Cao, K.F.: The heterogeneity and spatial patterning of structure and physiology across the leaf surface in giant leaves of Alocasia macrorrhiza. - PloS One 8: e66016, 2013. Go to original source...
    9. Lian, H.L., Yu, X., Lane, D., Sun, W.N., Tang, Z.C., Su, W.A.: Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment. - Cell. Res. 16: 651-660, 2006. Go to original source...
    10. Lichtenthaler, H.K..: Vegetation stress: an introduction to the stress concept in plants. - J. Plant Physiol. 148: 4-14, 1996. Go to original source...
    11. Massacci, A., Nabiev, S.M., Pietrosanti, L., Nematov, S.K., Chernikova, T.N., Thor, K., Leipner, J.: Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging. - Plant Physiol. Biochem. 46: 189-195, 2008. Go to original source...
    12. Meng, Q.W., Siebke, K., Lippert, P., Baur, B., Mukherjee, U., Weis, E.: Sink-source transition in tobacco leaves visualized using chlorophyll fluorescence imaging. - New Phytol. 151: 585-595, 2001. Go to original source...
    13. Osmond, C.B., Kramer, D., Lüttge, U.: Reversible, water stress-induced non-uniform chlorophyll fluorescence quenching in wilting leaves of Potentilla reptans may not be due to patchy stomatal responses. - Plant. Biol. 1: 618-624, 1999. Go to original source...
    14. Oxborough, K., Baker, N.R.: Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical componentscalculation of qP and Fv'/Fm' without measuring Fo'. - Photosynth. Res. 54: 135-142, 1997. Go to original source...
    15. Pal, A.K., Acharya, K., Vats, S.K., Kumar, S., Ahuja, P.S.: Over-expression of PaSOD in transgenic potato enhances photosynthetic performance under drought. - Biol. Plant. 57: 359-364, 2013. Go to original source...
    16. Pereira, E.G., Oliva, M.A., Rosado-Souza, L., Mendes, G.C., Colares, D.S., Stopato, C.H., Almeida, A.M.: Iron excess affects rice photosynthesis through stomatal and nonstomatal limitations. - Plant. Sci. 201-202: 81-92, 2013. Go to original source...
    17. Pietrini, F., Zacchini, M., Iori, V., Pietrosanti, L., Ferretti, M., Massacci, A.: Spatial distribution of cadmium in leaves and its impact on photosynthesis: examples of different strategies in willow and poplar clones. - Plant. Biol. 12: 355-363, 2010. Go to original source...
    18. Qu, Y.Y., Mu, P., Li, X.Q., Tian, Y.X., Wen, F., Zhang, H.L., Li, Z.C.: QTL mapping and correlations between leaf water potential and drought resistance in rice under upland and lowland environments. - Acta agron. sin. 34: 198-206, 2008. Go to original source...
    19. Shaikh, N.U., Quick, W.P., Rolfe, S.A.: The base of the leaf acts as a localized sink for photosynthate in mature barley leaves. - New Phytol. 148: 257-266, 2000. Go to original source...
    20. Siebke, K., Weis, E.: Assimilation images of leaves of Glechoma hederacea: analysis of non-synchronous stomata related oscillations. - Planta 196: 155-165, 1995a. Go to original source...
    21. Siebke, K., Weis, E.: Imaging of chlorophyll-a-fluorescence in leaves: topography of photosynthetic oscillations in leaves of Glechoma hederacea. - Photosynth. Res. 45: 225-237, 1995b. Go to original source...
    22. Song, J., Yang, Y.Q., Zhu, S.H., Chen, G.C., Yuan, X.F., Liu, T.T., Yu, X.H., Shi, J.Y.: Spatial distribution and speciation of copper in root tips of cucumber revealed by μ-XRF and μ-XANES. - Biol. Plant. 57: 581-586, 2013. Go to original source...
    23. Sperdouli, I., Moustakas, M.: Spatio-temporal heterogeneity in Arabidopsis thaliana leaves under drought stress. - Plant Biol. 14: 118-128, 2012. Go to original source...
    24. Wingler, A., Marès, M., Pourtau, N.: Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence. - New Phytol. 161: 781-789, 2004. Go to original source...

    Return to the content