biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 59:463-468, 2015 | DOI: 10.1007/s10535-015-0526-x

Generation of expressed sequence tags from a cDNA library of Coleus forskohlii for identification of genes involved in terpene biosynthesis

Y. Fang1,2, J. Huang1, X. Huang1, S. H. Chen2, P. C. Zou3, W. S. Li3, K. Yu1,2,3,*, Y. W. Liu1,2,*
1 College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P.R. China
2 Key Laboratory of Traditional Chinese Medicine Resource and Chemistry, Hubei University of Chinese Medicine, Wuhan, P.R. China
3 Postdoctoral Research Center, Furen Pharmaceutical Co., Tongcheng, P.R. China

Coleus forskohlii (syn. Plectranthus barbatus) is a widely used medicinal plant and its main bioactive constituents are diterpenes forskolin and isoforskolin. The present study aimed to construct a cDNA library to identify expressed sequence tags related to terpene biosynthesis in C. forskohlii. We constructed a high quality normalized full-length cDNA library which reached the requirements (abundance, integrity, and library content) for isolating full-length genes. A total of 4 224 cDNA clones were sequenced and 2 394 unigenes were assembled with an average unigene size of 753 bp. A total of 2 100 (87.7 %) unigenes were functionally classified using gene ontologies, and 1 716 (71.7 %) unigenes were assigned to establish pathway associations in KEGG mapings. Notably, 64 unigenes putatively participated in the biosynthesis of secondary metabolites, in which 17 unigenes were identified that might be involved in the biosynthesis of the terpenoid backbone and monoterpenes, diterpenes, and triterpenes.

Keywords: forskolin; isoforskolin; terpene backbone
Subjects: expressed sequence tags; terpene biosynthesis; forskolin; cDNA library; isoforskolin

Received: November 5, 2013; Revised: February 26, 2015; Accepted: April 7, 2015; Published: September 1, 2015  Show citation

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Fang, Y., Huang, J., Huang, X., Chen, S.H., Zou, P.C., Li, W.S., Yu, K., & Liu, Y.W. (2015). Generation of expressed sequence tags from a cDNA library of Coleus forskohlii for identification of genes involved in terpene biosynthesis. Biologia plantarum, 59(3), 463-468. doi: 10.1007/s10535-015-0526-x
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