biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 59:570-580, 2015 | DOI: 10.1007/s10535-015-0519-9

NADPH oxidase RBOHD contributes to autophagy and hypersensitive cell death during the plant defense response in Arabidopsis thaliana

H. B. Liu1,2,3, X. D. Wang1, Y. Y. Zhang3, J. J. Dong1,2, C. Ma1,2, W. L. Chen1,*
1 MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China
2 Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, P.R. China
3 Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, P.R. China

Autophagy has been implicated as a cellular protein degradation process that is used to recycle cytoplasmic components under biotic and abiotic stresses and so restrict programmed cell death (PCD). In this study, we report a novel regulatory mechanism by which NADPH oxidase respiratory burst oxidase homolog D (RBOHD) regulated pathogen-induced autophagy and hypersensitive (HR) cell death. We found that the Pseudomonas syringae pv tomato bacteria DC3000 expressing avrRps4 (Pst-avrRps4) induction of RBOHD-dependent reactive oxygen species (ROS) production promoted the onset of autophagy, whereas a pretreatment with an NADPH oxidase RBOHD inhibitor reversed this trend. The inhibitor significantly blocked pathogen-induced autophagosome formation and ROS increase. Moreover, we also show that in the wild-type and atrbohF mutant, Pst-avrRps4-induced cell death was limited, whereas in the case of the atrbohD mutant, the infection triggered a spreading-type necrosis. Our results demonstrate that the RBOHD-dependent ROS accumulation stimulated autophagosome formation and limited HR cell death.

Keywords: mutants; pathogen resistance; programmed cell death; Pst-avrRps4
Subjects: autophagy; hypersensitive reaction; pathogen resistance; NADPH oxidase; mutants; gene expression

Received: July 15, 2014; Revised: January 2, 2015; Accepted: January 8, 2015; Published: September 1, 2015  Show citation

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Liu, H.B., Wang, X.D., Zhang, Y.Y., Dong, J.J., Ma, C., & Chen, W.L. (2015). NADPH oxidase RBOHD contributes to autophagy and hypersensitive cell death during the plant defense response in Arabidopsis thaliana. Biologia plantarum, 59(3), 570-580. doi: 10.1007/s10535-015-0519-9
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