biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:25-36, 2016 | DOI: 10.1007/s10535-015-0558-2

Precise karyotyping of carrot mitotic chromosomes using multicolour-FISH with repetitive DNA

A. Nowicka1, E. Grzebelus2, D. Grzebelus2,*
1 Department of Cell Biology, Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, Krakow, Poland
2 Department of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Krakow, Poland

Carrot (Daucus carota L.) chromosomes are small and uniform in shape and length. Here, mitotic chromosomes were subjected to multicolour fluorescence in situ hybridization (mFISH) with probes derived from conserved plant repetitive DNA (18-25S and 5S rDNA, telomeres), a carrot-specific centromeric repeat (Cent-Dc), carrot-specific repetitive elements (DCREs), and miniature inverted-repeat transposable elements (MITEs). A set of major chromosomal landmarks comprising rDNA and telomeric and centromeric sequences in combination with chromosomal measurements enabled discrimination of carrot chromosomes. In addition, reproducible and unique FISH patterns generated by three carrot genome-specific repeats (DCRE22, DCRE16, and DCRE9) and two transposon families (DcSto and Krak) in combination with telomeric and centromeric reference probes allowed identification of chromosome pairs and construction of detailed carrot karyotypes. Hybridization patterns for DCREs were observed as pericentromeric and interstitial dotted tracks (DCRE22), signals in pericentromeric regions (DCRE16), or scattered signals (DCRE9) along chromosomes similar to those observed for both MITE families.

Keywords: Daucus carota repetitive elements; fluorescence in situ hybridization; miniature inverted-repeat transposable elements
Subjects: karyotyping; FISH; repetitive DNA; carrot

Received: April 21, 2015; Revised: June 8, 2015; Accepted: June 18, 2015; Published: January 1, 2016  Show citation

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Nowicka, A., Grzebelus, E., & Grzebelus, D. (2016). Precise karyotyping of carrot mitotic chromosomes using multicolour-FISH with repetitive DNA. Biologia plantarum, 60(1), 25-36. doi: 10.1007/s10535-015-0558-2
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