biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 60:79-85, 2016 | DOI: 10.1007/s10535-015-0577-z

Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering

Z.-Z. Song1,2, B.-B. Zhang1,2, C.-H. Zhang1,2, R.-J. Ma1,2, M.-L. Yu1,2,*
1 Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, P.R. China
2 Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, P.R. China

Iron is required for the Fe-S cluster assembly which occurs in chloroplasts, mitochondria, and cytosol and here we characterized 44 Fe-S cluster biosynthesis genes and investigated their expression profiles during different peach flowering stages. Quantitative real-time PCR analysis shows that the highest expression of most peach Fe-S cluster biosynthesis genes appeared in the full bloom stage. Also, the highest Fe accumulation occurred in the full bloom stage followed by beginning bloom, petal fall, and bud swell stages. Activities of nitrite reductase (NiR) and succinate dehydrogenase (SDH) were closely correlated to the flower Fe content, whereas the aconitase (ACO) activity kept steady during the whole flowering process. Moreover, shading treatment significantly reduced Fe accumulation and NiR, SDH, and ACO activities of the full blooming flowers. Seventeen Fe-S cluster biosynthesis genes were down-regulated in response to a shading treatment. In particular, plastid sulfur mobilization genes were sensitive to the shading treatment.

Keywords: aconitase; Fe homeostasis; nitrite reductase; Prunus persica; shading; succinate dehydrogenase
Subjects: iron; sulfur; flowering; Fe-S cluster biosynthesis; gene expression; peach; shading

Received: January 27, 2015; Revised: July 17, 2015; Accepted: July 27, 2015; Published: January 1, 2016  Show citation

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Song, Z.-Z., Zhang, B.-B., Zhang, C.-H., Ma, R.-J., & Yu, M.-L. (2016). Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering. Biologia plantarum, 60(1), 79-85. doi: 10.1007/s10535-015-0577-z
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