biologia plantarum

Biologia plantarum 60:163-172, 2016 | DOI: 10.1007/s10535-015-0570-6

Glutathione metabolism in Urtica dioica in response to cadmium based oxidative stress

L. Tarhan^1,*, B. Kavakcioglu¹

¹ Faculty of Science, Department of Chemistry, University of Dokuz Eylul, Buca, Izmir, Turkey

To investigate the antioxidative response of glutathione metabolism in Urtica dioica L. to a cadmium induced oxidative stress, activities of glutathione reductase (GR), glutathione-S-transferase (GST), and glutathione peroxidase (GSH-Px), content of reduced (GSH) and oxidized (GSSG) glutathione, lipid peroxidation (LPO), and also accumulation of Fe, Zn, Mn, Cu besides Cd were determined in the roots, stems, and leaves of plants exposed to 0 (control), 0.045, and 0.09 mM CdCl₂ for 58 h. Whereas the Cd content continuously increased in all organs, the Fe, Zn, Mn, and Cu content decreased in dependence on the applied Cd concentration and incubation time. The Cd treatment resulted in increased GR and GST activities in all organs, however, GSH-Px activity was dependent on Cd concentration and plant organ. The GSH/GSSG ratio maintained above the control level in the stems at both Cd concentrations. The LPO was generally close to the control values in the roots and stems but it increased in the leaves especially at 0.09 mM Cd.

Keywords: glutathione peroxidase; glutathione reductase; glutathione-S-transferase; lipid peroxidation; nettle

Subjects: glutathione; cadmium; glutathione reductase; glutathione peroxidase; glutathione S-transferase; iron; zinc; manganese; copper; malondialdehyde; nettle

Received: December 16, 2014; Revised: May 24, 2015; Accepted: May 27, 2015; Published: January 1, 2016  Show citation