biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 60:469-481, 2016 | DOI: 10.1007/s10535-016-0616-4

Identification and characterization of a novel iron deficiency and salt stress responsive transcription factor IDEF1 in Porteresia coarctata

D. Purohit1, H. M. Sankararamasubramanian1, A. Kumar Pal1, A. Kumar Parida1,*
1 M.S. Swaminathan Research Foundation, Chennai, India

Iron availability affects plant growth depending on soil type. Mangroves are characterized by alkaline soils in which the halophytic wild rice relative Porteresia coarctata thrives. Young plants of P. coarctata grew optimally in the presence of 150 mM NaCl in a hydroponic medium and tolerated iron deficiency and salt up to 21 d without showing any symptoms of stress. A homolog of the rice iron deficiency responsive cis-acting element binding factor (IDEF1) that functions at the base of an iron regulated network was isolated and characterized from P. coarctata. PcIDEF1 had a close paralog in P. coarctata genome and its transcript expression was upregulated by both iron deficient conditions and salt treated conditions for up to three weeks. Sub-cellular localization study suggests nuclear targeting PcIDEF1 protein in guard cells and root tissues of tobacco. In vitro assays for metal binding affinity and binding PcIDEF1 to iron deficiency responsive element 1 (IDE1)-like elements in the 5' flanking region of an iron regulated transporter from P. coarctata suggest that PcIDEF1 could potentially sense iron content in a plant cell and regulate expression of iron responsive genes containing IDE1-like elements in their promoter region. This study provides evidence for a possible cross-talk between iron deficiency and salt responses.

Keywords: alkaline pH; iron transporter; NaCl; tobacco; wild rice
Subjects: transcription factor; iron deficiency; salinity; alkaline stress; chlorophyll; subcellular localization; metal binding; tobacco; wild rice

Received: June 24, 2015; Revised: October 27, 2015; Accepted: November 4, 2015; Published: September 1, 2016  Show citation

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Purohit, D., Sankararamasubramanian, H.M., Kumar Pal, A., & Kumar Parida, A. (2016). Identification and characterization of a novel iron deficiency and salt stress responsive transcription factor IDEF1 in Porteresia coarctata. Biologia plantarum, 60(3), 469-481. doi: 10.1007/s10535-016-0616-4
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