biologia plantarum

Biologia plantarum 60:505-512, 2016 | DOI: 10.1007/s10535-016-0609-3

Molecular characterization and phylogenetic analysis of Wx genes from three Taeniatherum diploid species

S. F. Dai¹, J. Q. Jiang¹, Y. N. Jia¹, X. F. Xue¹, D. C. Liu¹, Y. M. Wei¹, Y. L. Zheng¹, Z. H. Yan^1,*

¹ Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China

In wheat seeds, starch synthase I or the Waxy protein is an enzyme involved in amylose synthesis. The gene encoding this enzyme is Wx and in this study, eight novel Wx alleles were identified in three diploid Taeniatherum species. The variability of these alleles was evaluated, and their nucleotide sequences were compared with those of homologous alleles from wheat. Two types of Taeniatherum Wx alleles were detected in three diploid species Ta. caput-medusae, Ta. asperum, and Ta. crinitum. A phylogenetic analysis indicates that the Taeniatherum Wx alleles were more closely related to Wx alleles from Aegilops species with C, D, M, and U genomes than to Wx alleles of other species. These alleles represent a potential genetic resource that may be useful in wheat breeding programs.

Keywords: amylose; starch; Triticum aestivum; Waxy protein

Subjects: starch synthase; amylose; gene expression; phylogenetic tree; wheat relatives

Received: October 21, 2015; Revised: January 5, 2016; Accepted: January 18, 2016; Published: September 1, 2016  Show citation