biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:677-685, 2016 | DOI: 10.1007/s10535-016-0628-0

Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress

D.-Y. Yang1, N.-N. Ma1, Z.-M. Liu1, X.-C. Ma1, S.-J. Zhao1,*, Q.-W. Meng1,*
1 College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China

Ascorbate (AsA) is an important antioxidant that can scavenge reactive oxygen species to protect plant cells against oxidative stress. Guanosine 5'-diphosphate (GDP)-L-galactose phosphorylase (GGP) is a key enzyme in the AsA biosynthetic pathway. To investigate the functions of GGP in AsA synthesis and oxidative stress tolerance in tomato, antisense lines with a reduced expression of SlGGP were obtained. Photobleaching after treatment of leaf disks with methyl viologen was more severe in transgenic lines compared to wild type (WT) plants. Moreover, compared with the WT plants, the transgenic plants showed a higher content of hydrogen peroxide, superoxide anion, malondialdehyde, as well as ion leakage, but a lower content of AsA and chlorophylls, ascorbate peroxidase activity, net photosynthetic rate, and maximal photochemical efficiency of photosystem II. Results of real-time quantitative polymerase chain reaction show that suppression of the SlGGP gene in the transgenic plants reduced their oxidative stress tolerance.

Keywords: ascorbate; ascorbate peroxidase; chlorophyll; GDP-L-galactose phosphorylase; malondialdehyde; net photosynthetic rate; photosystem II; Solanum lycopersicum
Subjects: oxidative stress; methyl viologen; malondialdehyde; electrolyte leakage; net photosynthetic rate; chlorophyll content; chlorophyll fluorescence; ascorbate peroxidase; catalase; superoxide dismutase; tomato

Received: July 23, 2015; Revised: December 23, 2015; Accepted: January 22, 2016; Published: December 1, 2016  Show citation

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Yang, D.-Y., Ma, N.-N., Liu, Z.-M., Ma, X.-C., Zhao, S.-J., & Meng, Q.-W. (2016). Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress. Biologia plantarum, 60(4), 677-685. doi: 10.1007/s10535-016-0628-0
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