biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:284-292, 2017 | DOI: 10.1007/s10535-016-0701-8

Function of Malus prunifolia WRKY6 transcription factor in response to different stresses

N. Wang1, Z.-Y. Yue1, P. Wang1, X. Sun1, X.-Q. Gong1, F.-W. Ma1,*
1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, P.R. China

The WRKY transcription factors (TFs) are integral parts of signaling pathways that regulate many processes, such as senescence, seed dormancy, seed germination, and resistance to abiotic and biotic stresses. Stress-related functions of WRKY6 have been characterized in Arabidopsis and other plant species, but its role has not been identified in apple. Here, we cloned WRKY6 genes from Malus prunifolia. Two homologues MpWRKY6a and MpWRKY6b found in this species were members of Group II WRKY6 TFs. They were localized to the cell nucleus. MpWRKY6a can bind to W-boxes. Compared with the untransformed wild type plants, MpWRKY6a-overexpressing Arabidopsis plants were more sensitive to methyl jasmonate (MeJA) and less sensitive to methyl viologen and abscisic acid (ABA), which suggests its role in responses to oxidative stress and MeJA or ABA signaling. The results fill a gap in the WRKY6 function in apple and provide basis for resistance improvement of Malus.

Keywords: abscisic acid; methyl jasmonate; methyl viologen; open reading frame; ubiquitin-like protein
Subjects: WRKY genes; transcription factor; abscisic acid; methyl jasmonate; methyl viologen; ubiquitin-like protein; yeast hybrid assay; sub-cellular localization; gene expression; transgenic plant

Received: May 11, 2016; Revised: August 12, 2016; Accepted: September 2, 2016; Published: June 1, 2017  Show citation

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Wang, N., Yue, Z.-Y., Wang, P., Sun, X., Gong, X.-Q., & Ma, F.-W. (2017). Function of Malus prunifolia WRKY6 transcription factor in response to different stresses. Biologia plantarum, 61(2), 284-292. doi: 10.1007/s10535-016-0701-8
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    References

    1. Besseau, S., Li, J, Palva, E.T.: WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. - J. exp. Bot. 63: 2667-2679, 2012. Go to original source...
    2. Castrillo, G., Sánchez-Bermejo, E., De Lorenzo, L., Crevillén, P., Fraile-Escanciano, A., Mohan, T.C., M., Mouriz, A., Catarecha, P., Sobrino-Plata, J., Olsson, S., Leo Del Puerto, Y., Mateos, I., Rojo, E., Hernández, L.E., Jarillo, J.A., Piñeiro, M., Paz-Ares, J., Leyva, A.: WRKY6 transcription factor restricts arsenate uptake and transposon activation in Arabidopsis. - Plant Cell 25: 2944-2957, 2013. Go to original source...
    3. Chen, H., Lai, Z.B., Shi, J.W., Xiao, Y., Chen, Z.X., Xu, X.P.: Roles of Arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. - BMC Plant Biol. 10: 281, 2010. Go to original source...
    4. Chen, L.G., Song, Y., Li, S.J., Zhang, L.P., Zou, C.S., Yu, D.Q.: The role of WRKY transcription factors in plant abiotic stresses. - Biochim. biophys. Acta 1819: 120-128, 2012. Go to original source...
    5. Chen, Y.F., Li, L.Q., Xu, Q., Kong, Y.H., Wang, H., Wu, W.H.: The WRKY6 transcription factor modulates PHOSPHATE1 expression in response to low Pi stress in Arabidopsis. - Plant Cell 21: 3554-3566, 2009. Go to original source...
    6. Ciolkowski, I., Wanke, D., Birkenbihl, R.P., Somssich, I.E.: Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function. - Plant mol. Biol. 68: 81-92, 2008. Go to original source...
    7. Eulgem, T., Somssich, I.E.: Networks of WRKY transcription factors in defense signaling. - Curr. Opin. Plant Biol. 10: 366-371, 2007. Go to original source...
    8. Eulgem, T., Rushton, P.J., Robatzek, S., Somssich, I.E.: The WRKY superfamily of plant transcription factors. - Trends Plant Sci. 5: 199-206, 2000. Go to original source...
    9. Farmer, L.M., Book, A.J., Lee, K.H., Lin, Y.L., Fu, H., Vierstra, R.D. The RAD23 family provides an essential connection between the 26S proteasome and ubiquitylated proteins in Arabidopsis. - Plant Cell 22: 124-142, 2010.
    10. Gambino, G., Perrone, I., Gribaudo, I. A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. - Phytochem. Anal. 19: 520-525, 2008. Go to original source...
    11. Giacomelli, J.I., Weigel, D., Chan, R.L., Manavella, P.A.: Role of recently evolved miRNA regulation of sunflower HaWRKY6 in response to temperature damage. - New Phytol. 195: 766-773, 2012. Go to original source...
    12. Guo, R.Y., Yu, F.F., Gao, Z., An, H.L., Cao, X.C., Guo, X.Q.: GhWRKY3, a novel cotton (Gossypium hirsutum L.) WRKY gene, is involved in diverse stress responses. - Mol. Biol. Rep. 38: 49-58, 2011. Go to original source...
    13. He, H.S., Dong, Q., Shao, Y.H., Jiang, H.Y., Zhu, S.W., Cheng, B.J., Xiang, Y.: Genome-wide survey and characterization of the WRKY gene family in Populus trichocarpa. - Plant Cell Rep. 31: 1199-1217, 2012. Go to original source...
    14. Hu, Y.R., Dong, Q.Y., Yu, D.Q.: Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae. - Plant Sci. 185-186: 288-297, 2012. Go to original source...
    15. Huang, S.X., Gao, Y.F., Liu, J.K., Peng, X.L., Niu, X.L., Fei, Z.J., Cao, S.Q., Liu, Y.S.: Genome-wide analysis of WRKY transcription factors in Solanum lycopersicum. - Mol. Genet. Genom. 287: 495-513, 2012. Go to original source...
    16. Huang, Y., Feng, C.Z., Ye, Q., Wu, W.H., Chen Y.F.: Arabidopsis WRKY6 transcription factor acts as a positive regulator of abscisic acid signaling during seed germination and early seedling development. - PloS Genetics 12: e1005833, 2016. Go to original source...
    17. Hwang, S.H., Yie, S.W., Hwang, D.J.: Heterologous expression of OsWRKY6 gene in Arabidopsis activates the expression of defense related genes and enhances resistance to pathogens. - Plant Sci. 181: 316-323, 2011. Go to original source...
    18. Ishiguro, S., Nakamura, K.: Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5' upstream regions of genes coding for sporamin and β-amylase from sweet potato. - Mol. gen. Genet. 244: 563-571, 1994. Go to original source...
    19. Kasajima, I., Ide, Y., Hirai, M.Y., Fujiwara, T.: WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana. - Physiol. Plant. 139: 80-92, 2010. Go to original source...
    20. Li, H.E., Xu, Y., Xiao, Y., Zhu, Z.G., Xie, X.Q., Zhao, H.Q., Wang, Y.J.: Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata. - Planta 232: 1325-1337, 2010. Go to original source...
    21. Li, H.L., Zhang, L.B., Guo, D., Li, C.Z., Peng, S.Q.: Identification and expression profiles of the WRKY transcription factor family in Ricinus communis. - Gene 503: 248-253, 2012. Go to original source...
    22. Li, P.L., Song, A.P., Gao, C.Y., Wang, L.X., Wang, Y.J., Sun, J., Jiang, J.F., Chen, F.D., Chen, S.M.: Chrysanthemum WRKY gene CmWRKY17 negatively regulates salt stress tolerance in transgenic chrysanthemum and Arabidopsis plants. - Plant Cell Rep. 34: 1365-1378, 2015. Go to original source...
    23. Livak, K., Schmittgen, T.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. - Methods 25: 402-408, 2001. Go to original source...
    24. Mao, G.H., Meng, X.Z., Liu, Y.D., Zheng, Z.Y., Chen, Z.X., Zhang, S.Q.: Phosphorylation of a WRKY transcription factor by two pathogen-responsive MAPKs drives phytoalexin biosynthesis in Arabidopsis. - Plant Cell 23: 1639-1653, 2011. Go to original source...
    25. Miao. Y., Zentgraf, U.: A HECT E3 ubiquitin ligase negatively regulates Arabidopsis leaf senescence through degradation of the transcription factor WRKY53. - Plant J. 63: 179-188, 2010. Go to original source...
    26. Niu, C.F., Wei, W., Zhou, Q.Y., Tian, A.G., Hao, Y.J., Zhang, W.K., Ma, B., Lin, Q., Zhang, Z.B., Zhang, J.S., Chen, S.Y.: Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants. - Plant Cell Environ. 35: 1156-1170, 2012. Go to original source...
    27. Pandey, S.P., Somssich, I.E. The role of WRKY transcription factors in plant immunity. - Plant Physiol. 150: 1648-1655, 2009. Go to original source...
    28. Porebski, S., Bailey, L.G., Baum, B.R.: Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. - Plant mol. Biol. Rep. 15: 8-15, 1997. Go to original source...
    29. Postel, D., Vanlemmens, P., Gode, P., Ronco, G., Villa, P.: PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. - Nucl. Acids Res. 30: 325-327, 2002. Go to original source...
    30. Ramamoorthy, R., Jiang, S.Y., Kumar, N., Venkatesh, P.N., Ramachandran, S.: A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. - Plant Cell Physiol. 49: 865-879, 2008. Go to original source...
    31. Robatzek, S., Somssich, I.E.: A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence-and defence-related processes. - Plant J. 28: 123-133, 2001. Go to original source...
    32. Rombauts, S., Déhais, P., Van Montagu, M., Rouzé, P.: PlantCARE, a plant cis-acting regulatory element database. - Nucl. Acids Res. 27: 295-296, 1999. Go to original source...
    33. Ross, C.A., Liu, Y., Shen, Q.J.: The WRKY gene family in rice (Oryza sativa). J. Integr. Plant. Biol. 49: 827-842, 2007. Go to original source...
    34. Rushton, D.L., Tripathi, P., Rabara, R.C., Lin, J., Ringler, P., Boken, A.K., Langum, T.J., Smidt, L., Boomsma, D.D., Emme, N.J., Chen, X., Finer, J.J., Shen, Q.J., Rushton, P.J: WRKY transcription factors: key components in abscisic acid signaling. - Plant Biotechnol. J. 10: 2-11, 2012. Go to original source...
    35. Rushton, P.J., Somssich, I.E., Ringler, P., Shen, Q.J.: WRKY transcription factors. - Trends Plant Sci. 15: 247-258, 2010. Go to original source...
    36. Skibbe, M., Qu, N., Galis, I., Baldwin, I.T.: Induced plant defenses in the natural environment: Nicotiana attenuata WRKY3 and WRKY6 coordinate responses to herbivory. - Plant Cell 20: 1984-2000, 2008. Go to original source...
    37. Song, Y., Chen, L.G., Zhang, L.P., Yu, D.Q.: Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis. - J. Biosci. 35: 459-471, 2010. Go to original source...
    38. Van Verk. M.C., Bol. J.F., Linthorst. H.J.: WRKY transcription factors involved in activation of SA biosynthesis genes. - BMC. Plant Biol. 11: 89, 2011. Go to original source...
    39. Wang S.C., Liang D., Shi S.G., Ma F.W., Shu H.R., Wang R.C.: Isolation and characterization of a novel drought responsive gene encoding a Glycine-rich RNA-binding protein in Malus prunifolia (Willd.) Borkh. - Plant mol. Biol. Rep. 29: 125-134, 2011. Go to original source...
    40. Wei, K.F., Chen, J., Chen, Y.F., Wu, L.J., Xie, D.X.: Molecular phylogenetic and expression analysis of the complete WRKY transcription factor family in maize. - DNA Res. 19: 153-164, 2012. Go to original source...
    41. Zhang, J.Y., Tong, Z.G., Gao, Z.H., Luo, C.G., Qu, S.C., Zhang, Z.: Expression of MhWRKY1 gene induced by the elicitors SA, MeJA, ACC and the apple ring spot pathogen. - Scientia Agr. 44: 990-999, 2011.
    42. Zhang, X., Henriques, R., Lin, S., Niu, Q., Chua, N.: Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. - Nat. Protocols 1: 641-646, 2006. Go to original source...
    43. Zhang, Y.J., Wang, L.J.: The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. - BMC Evol. Biol. 5: 1, 2005. Go to original source...
    44. Zhou, X., Jiang, Y.J., Yu, D.Q.: WRKY22 transcription factor mediates dark-induced leaf senescence in Arabidopsis. - Mol. Cells 31: 303-313, 2011. Go to original source...

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