biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:323-332, 2017 | DOI: 10.1007/s10535-016-0663-x

Enhancement of stress tolerance in cucumber seedlings by proanthocyanidins

L.-J. Zhu1,2, X.-G. Deng1, L.-J. Zou1,2, D.-W. Zhang1,*, H.-H. Lin1,*
1 Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, P.R. China
2 Ecological Security and Protection Key Laboratory of Sichuan Province, MianYang Teachers' College, Mianyang, P.R. China

Proanthocyanidins (PAs) are the main products of the flavonoid biosynthetic pathway in many plants. However, their biological function during environmental stresses in plants is rarely reported. In the present study, the effects of pretreatment with PAs on the response of cucumber (Cucumis sativus L.) seedlings to high irradiance (HI), polyethylene glycol (PEG), and cold stress were investigated. The PAs pretreament alleviated stress-induced oxidative damage in plant cells and increased the activity of alternative oxidase (AOX) and content of abscisic acid (ABA). Furthermore, PAs-pretreated seedlings suffered less damage by the stress conditions, maintained higher content of chlorophyll a+b and AOX proteins in comparison with the control. Therefore, our findings suggest that PAs might contribute to plant tolerance to environmental stresses.

Keywords: abscisic acid; alternative oxidase; chlorophyll; cold; Cucumis sativus; high irradiance; PEG
Subjects: proanthocyanidins; stress tolerance; abscisic acid; alternative oxidase; electrolyte leakage; polyethylene glycol; high irradiance; cold stress; catalase; ascorbate peroxidase; superoxide dismutase; chlorophyll content; respiration rate; cucumber

Received: November 1, 2015; Revised: March 21, 2016; Accepted: April 1, 2016; Published: June 1, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Zhu, L.-J., Deng, X.-G., Zou, L.-J., Zhang, D.-W., & Lin, H.-H. (2017). Enhancement of stress tolerance in cucumber seedlings by proanthocyanidins. Biologia plantarum, 61(2), 323-332. doi: 10.1007/s10535-016-0663-x
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download filebpl-201702-0014_S1.pdf

    File size: 2.13 MB

    References

    1. Bahin, E., Bailly, C., Sotta, B., Kranner, I., Corbineau, F., Leymarie, J.: Crosstalk between reactive oxygen species and hormonal signalling pathways regulates grain dormancy in barley. - Plant Cell Environ. 34: 980-993, 2011. Go to original source...
    2. Cao, Y., Zhang, Z.W., Xue, L.W., Du, J.B., Shang, J., Xu, F., Yuan, S., Lin, H.H.: Lack of salicylic acid in Arabidopsis protects plants against moderate salt stress. - Z. Naturforsch. 64c: 231-238, 2009. Go to original source...
    3. Dixon, R.A., Xie, D.Y., Sharma, S.B.: Proanthocyanidins-a final frontier in flavonoid research? - New Phytol. 165: 9-28, 2005. Go to original source...
    4. Dong, C.J., Li, L., Shang, Q.M., Liu, X.Y., Zhang, Z.G.: Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings. - Planta 240: 687-700, 2014. Go to original source...
    5. Du, J.B., Yuan, S., Chen, Y.E., Sun, X., Zhang, Z.W., Xu, F., Yuan, M., Shang, J., Lin, H.H.: Comparative expression analysis of dehydrins between two barley varieties, wild barley and Tibetan hulless barley associated with different stress resistance. - Acta Physiol. Plant. 33: 567-574, 2011. Go to original source...
    6. Foyer, C.H., Noctor, G.: Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications. - Antioxid. Redox Signal. 11: 861-905, 2009. Go to original source...
    7. Galvez-Valdivieso, G., Fryer, M.J., Lawson, T., Slattery, K., Truman, W., Smirnoff, N., Asami, T., Davies, W.J., Jones, A.M., Baker, N.R.: The high light response in Arabidopsis involves ABA signaling between vascular and bundle sheath cells. - Plant Cell 21: 2143-2162, 2009. Go to original source...
    8. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - B.B.A. Gen. Subjects 990: 87-92, 1989. Go to original source...
    9. Gilbert, N.: Drought devastates US crops. - Nature doi:10.1038/nature.2012.11065, 2012. Go to original source...
    10. Giraud, E., Ho, L.H.M., Clifton, R., Carroll, A., Estavillo, G., Tan, Y.F., Howell, K.A., Ivanova, A., Pogson, B.J., Millar, A.H.: The absence of alternative oxidase1a in Arabidopsis results in acute sensitivity to combined light and drought stress. - Plant Physiol. 147: 595-610, 2008. Go to original source...
    11. Gould, K.S., Dudle, D.A., Neufeld, H.S.: Why some stems are red: cauline anthocyanins shield photosystem II against high light stress. - J. exp. Bot. 61: 2707-2717, 2010. Go to original source...
    12. Jia, L.G., Wu, Q.Y., Ye, N.H., Liu, R., Shi, L., Xu, W.F., Zhi, H., Rahman, A., Xia, Y.J., Zhang, J.H.: Proanthocyanidins inhibit seed germination by maintaining a high level of abscisic acid in Arabidopsis thaliana. - J. integr. Plant Biol. 54: 663-673, 2012. Go to original source...
    13. Julliard, J., Maldiney, R., Sotta, B., Miginiac, E., Kerhoas, L., Einhorn, J.: HPLC-ELISA and MS as complementary techniques to study plant hormone metabolites. - Analysis 22: 483-489, 1994.
    14. Kusano, M., Tohge, T., Fukushima, A., Kobayashi, M., Hayashi, N., Otsuki, H., Kondou, Y., Goto, H., Kawashima, M., Matsuda, F.: Metabolomics reveals comprehensive reprogramming involving two independent metabolic responses of Arabidopsis to UV-B light. - Plant J. 67: 354-369, 2011. Go to original source...
    15. Larkindale, J., Knight, M.R.: Protection against heat stressinduced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. - Plant Physiol. 128: 682-695, 2002. Go to original source...
    16. Lee, Y.P., Baek, K.H., Lee, H.S., Kwak, S.S., Bang, J.W., Kwon, S.Y.: Tobacco seeds simultaneously over-expressing Cu/Zn-superoxide dismutase and ascorbate peroxidase display enhanced seed longevity and germination rates under stress conditions. - J. exp. Bot. 61: 2499-2506, 2010. Go to original source...
    17. Lei, T., Feng, H., Sun, X., Dai, Q.L., Zhang, F., Liang, H.G., Lin, H.H.: The alternative pathway in cucumber seedlings under low temperature stress was enhanced by salicylic acid. - Plant Growth Regul. 60: 35-42, 2010. Go to original source...
    18. Lu, S., Su, W., Li, H., Guo, Z.: Abscisic acid improves drought tolerance of triploid bermuda grass and involves H2O2-and NO-induced antioxidant enzyme activities. - Plant Physiol. Biochem. 47: 132-138, 2009. Go to original source...
    19. Maxwell, D.P., Wang, Y., McIntosh, L.: The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. - Proc. nat. Acad. Sci. USA 96: 8271-8276, 1999. Go to original source...
    20. Mittler, R.: Oxidative stress, antioxidants and stress tolerance. - Trends Plant Sci. 7: 405-410, 2002. Go to original source...
    21. Moller, I.M.: Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. - Annu. Rev. Plant Biol. 52: 561-591, 2001. Go to original source...
    22. Moller, I.M., Berczi, A., Van der Plas, L.H.W., Lambers, H.: Measurement of the activity and capacity of the alternative pathway in intact plant tissues: identification of problems and possible solutions. - Physiol. Plant. 72: 642-649, 1988. Go to original source...
    23. Moller, I.M., Sweetlove, L.J.: ROS signalling-specificity is required. - Trends Plant Sci. 15: 370-374, 2010. Go to original source...
    24. Nakabayashi, R., Yonekura-Sakakibara, K., Urano, K., Suzuki, M., Yamada, Y., Nishizawa, T., Matsuda, F., Kojima, M., Sakakibara, H., Shinozaki, K.: Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. - Plant J. 77: 367-379, 2014. Go to original source...
    25. Page, M., Sultana, N., Paszkiewicz, K., Florance, H., Smirnoff, N.: The influence of ascorbate on anthocyanin accumulation during high light acclimation in Arabidopsis thaliana: further evidence for redox control of anthocyanin synthesis. - Plant Cell Environ. 35: 388-404, 2012. Go to original source...
    26. Popov, V.N., Simonian, R.A., Skulachev, V.P., Starkov, A.A.: Inhibition of the alternative oxidase stimulates H2O2 production in plant mitochondria. - FEBS Lett. 415: 87-90, 1997. Go to original source...
    27. Stewart, R.R., Bewley, J.D.: Lipid peroxidation associated with accelerated aging of soybean axes. - Plant Physiol. 65: 245-248, 1980. Go to original source...
    28. Stracke, R., Favory, J.J., Gruber, H., Bartelniewoehner, L., Bartels, S., Binkert, M., Funk, M., Weisshaar, B., Ulm, R.: The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation. - Plant Cell Environ. 33: 88-103, 2010. Go to original source...
    29. Van Aken, O., Giraud, E., Clifton, R., Whelan, J.: Alternative oxidase: a target and regulator of stress responses. - Physiol. Plant. 137: 354-361, 2009. Go to original source...
    30. Varshney, R.K., Ribaut, J.M., Buckler, E.S., Tuberosa, R., Rafalski, J.A., Langridge, P.: Can genomics boost productivity of orphan crops? - Nat. Biotechnol. 30: 1172-1176, 2012. Go to original source...
    31. Velikova, V., Yordanov, I., Edreva, A.: Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. - Plant Sci. 151: 59-66, 2000. Go to original source...
    32. Wang, S.D., Zhu, F., Yuan, S., Yang, H., Xu, F., Shang, J., Xu, M.Y., Jia, S.D., Zhang, Z.W., Wang, J.H., Xi, D.H., Lin, H.H.: The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses. - Planta 234: 171-181, 2011. Go to original source...
    33. Xi, D.M., Liu, W.S., Yang, G.D., Wu, C.A., Zheng, C.C.: Seedspecific overexpression of antioxidant genes in Arabidopsis enhances oxidative stress tolerance during germination and early seedling growth. - Plant Biotechnol. J. 8: 796-806, 2010. Go to original source...
    34. Xu, F., Zhang, D.W., Zhu, F., Tang, H., Lv, X., Cheng, J., Xie, H.F., Lin, H.H.: A novel role for cyanide in the control of cucumber(Cucumis sativus L.) seedlings response to environmental stress. - Plant Cell Environ. 35: 1983-1997, 2012. Go to original source...
    35. Yoshida, K., Terashima, I., Noguchi, K.: Distinct roles of the cytochrome pathway and alternative oxidase in leaf photosynthesis. - Plant Cell Physiol. 47: 22-31, 2006. Go to original source...
    36. Zhang, D.W., Xu, F., Zhang, Z.W., Chen, Y.E., Du, J.B., Jia, S.D., Yuan, S., Lin, H.H.: Effects of light on cyanideresistant respiration and alternative oxidase function in Arabidopsis seedlings. - Plant Cell Environ. 33: 2121-2131, 2010. Go to original source...
    37. Zhang, K.M., Yu, H.J., Shi, K., Zhou, Y.H., Yu, J.Q., Xia, X.J.: Photoprotective roles of anthocyanins in Begonia semperflorens. - Plant Sci. 179: 202-208, 2010. Go to original source...

    Return to the content