biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:438-444, 2017 | DOI: 10.1007/s10535-016-0702-7

Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate

Z. Q. Yuan1, J. Y. Zhang1,*, T. Liu1
1 School of Life Sciences, Shandong University of Technology, Zibo, Shandong, P.R. China

The accumulation of polysaccharides, activities of sucrose metabolism enzymes, and the expression of sucrose biosynthetic genes in Dendrobium officinale were significantly affected by exogenous methyl jasmonate (MeJA). Application of MeJA increased the content of polysaccharides and the highest polysaccharide production occurred in the samples treated with 200 μM MeJa. The MeJA application influenced polysaccharide biosynthesis rather than degradation because the activities of sucrose metabolism enzymes and the expressions of sucrose biosynthetic genes were upregulated by MeJA. Interestingly, low MeJA concentrations promoted accumulation of Dendrobium polysaccharides, while high MeJA amounts played an inhibitory role. The content of major constituent of polysaccharides, glucose and mannose, also increased after MeJa treatment.

Keywords: gene expression; glucose; mannose; sucrose phosphate synthase; sucrose synthase
Subjects: in vitro culture; polysaccharides; glucose; mannose; sucrose phosphate synthase; sucrose synthase; gene expression; methyl jasmonate

Received: November 18, 2015; Revised: October 15, 2016; Accepted: October 17, 2016; Published: September 1, 2017  Show citation

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Yuan, Z.Q., Zhang, J.Y., & Liu, T. (2017). Enhancement of polysaccharides accumulation in Dendrobium officinale by exogenously applied methyl jasmonate. Biologia plantarum, 61(3), 438-444. doi: 10.1007/s10535-016-0702-7
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