biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:587-594, 2017 | DOI: 10.1007/s10535-017-0713-z

Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical composition

M. L. Strieder1,2,4, K. G. Pinto1,2, C. Bertoldi1, A. de B. Schneider1,3, C. A. Delatorre1,*
1 Department of Crop Science, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
2 Institute of Developmental Genetics, Heinrich Heine University, Düsseldorf, Germany
3 Department of Bioinformatics and Genomics, University of North Carolina, Charlotte, USA
4 Embrapa Wheat, Passo Fundo, Brazil

The interaction between phosphorus (P) and other media components alters root development and masks the plant response and thus limits the ability to correctly identify P-deficiency response (pdr) mutants. This study aims to assess changes in root development caused by different composition of growth media normally used in Arabidopsis research and to study their effects on pdr-mutant screening. Primary root growth of four genotypes was analyzed in media differing in P concentrations: half-strength Murashige and Skoog (1/2 MS) and Somerville and Ogren (SO). The effects of nitrogen source and Fe on root growth were investigated in each medium separately and in a mixture. We found that the primary root length of all genotypes grown on 1/2 MS was reduced in comparison with plants grown on SO medium. The mutant pdr9 was the most sensitive in 1/2 MS, This mutant was also hypersensitive to Fe that intensified its sensitivity to ammonium. Ammonium increased the root inhibition caused by Fe also in wild-type plants. In conclusion, on the basis of our study we recommend to use SO medium, which ensures an efficient selection to screen for pdr mutants through root growth. Moreover, nitrogen sources in the media other than nitrate should be taken carefully.

Keywords: ammonium sensitivity; iron hypersensitivity; nutrient interactions; phosphate deficiency; quiescent center identity
Subjects: in vitro culture; ammonium; iron; phosphorus; primary root growth; lateral root development; nitrate

Received: November 23, 2015; Revised: October 18, 2016; Accepted: October 21, 2016; Published: September 1, 2017  Show citation

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Strieder, M.L., Pinto, K.G., Bertoldi, C., de Schneider, A.B., & Delatorre, C.A. (2017). Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical composition. Biologia plantarum, 61(3), 587-594. doi: 10.1007/s10535-017-0713-z
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