biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:641-650, 2017 | DOI: 10.1007/s10535-017-0724-9

Na+/H+ and K+/H+ antiporters AtNHX1 and AtNHX3 from Arabidopsis improve salt and drought tolerance in transgenic poplar

L. Yang1,2, H. Liu3, S. M. Fu1, H. M. Ge1, R. J. Tang3, Y. Yang3, H. H. Wang3, H. X. Zhang2,3,*
1 College of Life Sciences, Nanjing University, Nanjing, P.R. China
2 College of Agriculture, Ludong University, Yantai, P.R. China
3 Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, P.R. China

The tonoplast and plasma membrane localized sodium (potassium)/proton antiporters have been shown to play an important role in plant resistance to salt stress. In this study, AtNHX1 and AtNHX3, two tonoplast Na+(K+)/H+ antiporter encoding genes from Arabidopsis thaliana, were expressed in poplar to investigate their biological functions in the resistance to abiotic stresses in woody plants. Transgenic poplar plants expressing either gene exhibited increased resistance to both salt and water-deficit stresses. Compared to the wild type (WT) plants, transgenic plants accumulated more sodium and potassium ions in the presence of 100 mM NaCl and showed reduced electrolyte leakage in the leaves under water stress. Furthermore, the proton-translocating and cation-dependent H+ (Na+/H+ or K+/H+) exchange activities in the tonoplast vesicles isolated from the leaves of transgenic plants were higher than in those isolated from WT plants. Therefore, constitutive expression of either AtNHX1 or AtNHX3 genetically modified the salt and water stress tolerance of transgenic poplar plants, providing a potential tool for engineering tree species with enhanced resistance to multiple abitotic stresses.

Keywords: abiotic stress; electrolyte leakage; Populus davidiana × Populus bolleana; tonoplast vesicles; transgenic plant; water deficit
Subjects: antiporters; salinity; drought; plasma membrane; tonoplast; electrolyte leakage; transgenic plants; sodium; potassium; poplar

Received: June 23, 2016; Revised: December 22, 2016; Accepted: December 30, 2016; Published: December 1, 2017  Show citation

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Yang, L., Liu, H., Fu, S.M., Ge, H.M., Tang, R.J., Yang, Y., Wang, H.H., & Zhang, H.X. (2017). Na+/H+ and K+/H+ antiporters AtNHX1 and AtNHX3 from Arabidopsis improve salt and drought tolerance in transgenic poplar. Biologia plantarum, 61(4), 641-650. doi: 10.1007/s10535-017-0724-9
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