biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:733-740, 2017 | DOI: 10.1007/s10535-017-0720-0

Zinc-alleviating effects on iron-induced phytotoxicity in roots of Triticum aestivum

T. Ma1, X. H. Duan1, Y. Y. Yang1,*, J. Yao1, T. P. Gao2
1 School of Life Science, Northwest Normal University, Lanzhou, P.R. China
2 Centre of Urban Ecology and Environmental Biotechnology, Lanzhou City University, Lanzhou, P.R. China

The mechanisms of growth inhibition and antioxidative response were investigated in wheat roots exposed to 300 μM iron together with different zinc concentrations (0, 50, and 250 μM). All Zn concentrations decreased Fe content but increased Zn content in the roots and leaves of Fe-treated seedlings. Compared with Fe stress alone, 50 or 250 μM Zn + Fe treatment stimulated root growth, and increased cell viability but decreased malondialdehyde content, which were correlated with the decreases of total and apoplastic hydrogen peroxide and superoxide anion radical (O2 .-) content along with apoplastic hydroxyl radical content. Generation of O2 .- in response to 10 μM diphenylene iodonium suggested that NADPH oxidase activity was lower in Zn + Fe-treated roots than in other roots. In addition, cell wallbound peroxidase, diamine oxidase, and polyamine oxidase in Fe-treated roots were insensitive to Zn addition. Further study showed the stimulation of total superoxide dismutase and glutathione reductase (GR) activities as well as apoplastic catalase, ascorbate peroxidase, and GR in Zn + Fe-stressed roots in comparison with Fe-alone-treated ones. Taken together, Zn could alleviate iron-inhibitory effect on root growth, which might be associated with the decrease of lipid peroxidation, the increase of cell viability and the reductions of reactive oxygen species generation.

Keywords: antioxidative enzymes; lipid peroxidation; reactive oxygen species; root growth
Subjects: phytotoxicity; zinc; iron; malondialdehyde; reactive oxygen species; peroxidase; catalase; diamine oxidase; polyamine oxidase; superoxide dismutase; ascorbate peroxidase; glutathione reductase; wheat

Received: September 5, 2015; Revised: June 6, 2016; Accepted: June 14, 2016; Published: December 1, 2017  Show citation

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Ma, T., Duan, X.H., Yang, Y.Y., Yao, J., & Gao, T.P. (2017). Zinc-alleviating effects on iron-induced phytotoxicity in roots of Triticum aestivum. Biologia plantarum, 61(4), 733-740. doi: 10.1007/s10535-017-0720-0
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