biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 62:129-139, 2018 | DOI: 10.1007/s10535-017-0742-7

Tolerance to soil water stress by Oryza sativa cv. IR20 was improved by expression of Wsi18 gene locus from Oryza nivara

R. Kaur1, A. Chakraborty1, R. K. Bhunia1, S. K. Sen1, A. K. Ghosh2,*
1 Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology-Kharagpur, Kharagpur, India
2 Department of Biotechnology, Indian Institute of Technology-Kharagpur, Kharagpur, India

Wild rice genotypes are rich in genetic diversity. This has potential to improve agronomic rice by allele mining for superior traits. Late embryogenesis abundant (LEA) proteins are often associated with desiccation tolerance and stress signalling. In the present study, a group 3 LEA gene, Wsi18 from the wild rice Oryza nivara was expressed under its own inducible promoter element in stress susceptible cultivated indica rice (cv. IR20). The resulting transgenic plants cultivated in a greenhouse showed enhanced tolerance to soil water deficit. Transgenic plants had higher grain yield, plant survival rate, and shoot relative water content compared to wild type (WT) IR20. Cell membrane stability index, proline and soluble sugar content were also greater in transgenic than WT plants under water stress. These results demonstrate the potential for improving SWS tolerance in agronomically important rice cultivar by incorporating Wsi18 gene from a wild rice O. nivara.

Keywords: inducible expression; electrolyte leakage; LEA proteins; proline; relative water content; transgenic plants
Subjects: drought tolerance; transgenic plants; sequence analysis; Southern blot; gene expression; proline; soluble sugars; electrolyte leakage; RWC; rice

Received: June 22, 2016; Revised: March 27, 2017; Accepted: March 28, 2017; Published: January 1, 2018  Show citation

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Kaur, R., Chakraborty, A., Bhunia, R.K., Sen, S.K., & Ghosh, A.K. (2018). Tolerance to soil water stress by Oryza sativa cv. IR20 was improved by expression of Wsi18 gene locus from Oryza nivara. Biologia plantarum, 62(1), 129-139. doi: 10.1007/s10535-017-0742-7
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    References

    1. Ashraf, M.: Inducing drought tolerance in plants: recent advances. - Biotechnol. Adv. 28: 169-183, 2010. Go to original source...
    2. Babu, R.C., Zhang, J., Blum, A., Davidho, T.H., Wu, R., Nguyen, H.: HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. - Plant Sci. 166: 855-862, 2004. Go to original source...
    3. Bajji, M., Kinet, J., Lutts, S.: The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. - Plant Growth Regul. 36: 61-70, 2002. Go to original source...
    4. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    5. Battaglia, M., Olvera-Carrillo, Y., Garciarrubio, A., Campos, F., Covarrubias, A.A.: The enigmatic LEA proteins and other hydrophilins. - Plant Physiol. 148: 6-24, 2008. Go to original source...
    6. Bhatnagar-Mathur, P., Vadez, V., Sharma, K.K.: Transgenic approaches for abiotic stress tolerance in plants: retrospect and prospects. - Plant Cell Rep. 27: 411-424, 2008. Go to original source...
    7. Bhattacharyya, J., Chowdhury, A.H., Ray, S., Jha, J.K., Das, S., Gayen, S., Chakraborty, A., Mitra, J., Maiti, M.K., Basu, A., Sen, S.K.: Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants. - Plant Cell Rep. 31: 271-279, 2012. Go to original source...
    8. Bhunia, R.K., Chakraborty, A., Kaur, R., Gayatri, T., Bhattacharyya, J., Basu, A., Maiti, M.K., Sen, S.K.: Seedspecific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops. - Plant mol. Biol. 86: 351-365, 2014. Go to original source...
    9. Bhunia, R.K., Chakraborty, A., Kaur, R., Maiti, M.K., Sen, S.K.: Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER. - Plant Cell Rep. 35: 213-226, 2016. Go to original source...
    10. Black C.A. (ed): Methods of Soil Analysis: Part 1, Physical and Mineralogical Properties. - American Society of Agronomy, Madison 1965. Go to original source...
    11. Checker, V.G., Chhibbar, A.K., Khurana, P.: Stress-inducible expression of barley Hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress. - Transgenic Res. 21: 939-957, 2012. Go to original source...
    12. Chen, Y.S., Lo, S.F., Sun, P.K., Lu, C.A., Ho, T.H.D., Yu, S.M.: A late embryogenesis abundant protein HVA1 regulated by an inducible promoter enhances root growth and abiotic stress tolerance in rice without yield penalty. - Plant Biotechnol. J. 13: 105-116, 2015. Go to original source...
    13. Dansana, P.K., Kothari, K.S., Vij, S., Tyagi, A.K.: OsiSAP1 overexpression improves water-deficit stress tolerance in transgenic rice by affecting expression of endogenous stress-related genes. - Plant Cell Rep. 33: 1425-1440, 2014. Go to original source...
    14. DeBuck, S., Van Montagu, M., Depicker, A.: Transgene silencing of invertedly repeated transgenes is released upon deletion of one of the transgenes involved. - Plant mol. Biol. 46: 433-445, 2001. Go to original source...
    15. DeBuck, S., Windels, P., De-Loose, M., Depicker, A.: Singlecopy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable betaglucuronidase accumulation levels. - Cell. Mol. Life Sci. 61: 2632-2645, 2004. Go to original source...
    16. Degenkolbe, T., Do, P.T., Zuther, E., Repsilber, D., Walther, D., Hincha, D.K., Kohl, K.I.: Expression profiling of rice cultivars differing in their tolerance to long-term drought stress. - Plant mol. Biol. 69: 133-153, 2009. Go to original source...
    17. Duan, J., Cai, W.: OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance. - PLoS ONE 7: e45117, 2012. Go to original source...
    18. Gao, J., Lan, T.: Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli. - Sci. Rep. 6: 19467, 2016. Go to original source...
    19. Gao, Y., Jiang, W., Dai, Y., Xiao, N., Zhang, C., Li, H., Lu, Y., Wu, M., Tao, X., Deng, D., Chen, J.: A maize phytochromeinteracting factor 3 improves drought and salt stress tolerance in rice. - Plant mol. Biol. 87: 413-428, 2015. Go to original source...
    20. Gaudin, A.C.M., Henry, A., Sparks, A.H., Slamet-Loedin, I.H.: Taking transgenic rice drought screening to the field. - J. exp. Bot. 64: 109-117, 2012. Go to original source...
    21. He, R.F.: Construction of a genomic library of Oryza officinalis and transformation of large DNA fragments. - PhD Thesis, Wuhan University, Wuhan 2003.
    22. Hundertmark, M., Hincha, D.K.: LEA (late embryogenesis abundant) proteins and their encoding genes in Arabidopsis thaliana. - BMC Genomics 9: 118, 2008. Go to original source...
    23. Hincha, D.K., Thalhammer, A.: LEA proteins: IDPs with versatile functions in cellular dehydration tolerance. - Biochem. Soc. Trans. 40: 1000-1003, 2012. Go to original source...
    24. Jeng, T. L., Tseng, T. H., Wang, C. S., Chen, C. L., Sung, J. M.: Yield and grain uniformity in contrasting rice genotypes suitable for different growth environments. - Field Crops Res. 99: 59-66, 2006. Go to original source...
    25. Joo, J., Lee, Y.H., Song, S.I.: Overexpression of the rice basic leucine zipper transcription factor OsbZIP12 confers drought tolerance to rice and makes seedlings hypersensitive to ABA. - Plant Biotechnol. Rep. 8: 431-441, 2014. Go to original source...
    26. Joshee, N., Kisaka, H., Kitagawa, Y.: Isolation and characterization of a water stress-specific genomic gene, pwsi 18, from rice. - Plant Cell Physiol. 39: 64-72, 1998. Go to original source...
    27. Kasuga, M., Miura, S., Shinozaki, K., Yamaguchi-Shinozaki, K.: A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought-and low-temperature stress tolerance in tobacco by gene transfer. - Plant Cell Physiol. 45: 346-350, 2004. Go to original source...
    28. Kaur, R., Bhunia, R.K., Ghosh, A.K.: Molecular genetic approaches for environmental stress tolerant crop plants: progress and prospects. - Recent Patents Biotechnol. 10: 12-29, 2016. Go to original source...
    29. Kaur, R., Chakraborty, A., Bhunia, R.K., Bhattacharyya, J., Basu, A., Sen, S.K., Ghosh, A.K.: Wsi18 promoter from wild rice genotype, Oryza nivara, shows enhanced expression under soil water stress in contrast to elite cultivar, IR20. - J. Plant Biochem. Biotech. 26: 14-26, 2017. Go to original source...
    30. Khurana, P., Vishnudasan, D., Chhibbar, A.K.: Genetic approaches towards overcoming water deficit in plantsspecial emphasis on LEAs. - Physiol. mol. Biol. Plants 14: 277-298, 2008. Go to original source...
    31. Kumar, G.R., Sakthivel, K., Sundaram, R.M., Neeraja, C.N., Balachandran, S.M., Rani, N.S., Viraktamath, B.C., Madhav, M.S.: Allele mining in crops: prospects and potentials. - Biotechnol. Adv. 28: 451-61, 2010. Go to original source...
    32. Lal, S., Gulyani, V., Khurana, P.: Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry (Morus indica). - Transgenic Res. 17: 651-663, 2008. Go to original source...
    33. Leung, H., Raghavan, C., Zhou, B., Oliva, R., Choi, R., Lacorte, V., Jubay, M.L., Cruz, C.V., Gregorio, G., Singh, R.K., Ulat, V.J., Borja, F.N., Mauleon, R., Alexandrov, N.N., McNally, K.L., Hamilton, R.S.: Allele mining and enhanced genetic recombination for rice breeding. - Rice 8: 34, 2015. Go to original source...
    34. Liang, X., Zhang, L., Natarajan, S.K., Becker, D.F.: Proline mechanisms of stress survival. - Antioxid. Redox. Signal. 19: 998-1011, 2013. Go to original source...
    35. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. - Methods 25: 402-408, 2001. Go to original source...
    36. Lu, B-R., Yang, C.: Gene flow from genetically modified rice to its wild relatives: assessing potential ecological consequences. - Biotechnol. Adv. 27: 1083-91, 2009. Go to original source...
    37. Maclean, J.L., Hardy, B., Hettel, G.P. (ed): Rice Almanac: Source Book for One of the Most Important Economic Activity on Earth. 4th Ed. - International Rice Research Institute, Los Banos 2013.
    38. Mickelbart, M.V., Hasegawa, P.M., Bailey-Serres, J.: Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. - Nat. Rev. Genet. 16: 237-251, 2015. Go to original source...
    39. Mittler, R., Blumwald, E.: Genetic engineering for modern agriculture: challenges and perspectives. - Annu. Rev. Plant Biol. 61: 443-462, 2010. Go to original source...
    40. Moons, A., Keyser, A.D., Montagu, M.V.: A group 3 LEA cDNA of rice, responsive to abscisic acid, but not to jasmonic acid, shows variety-specific differences in salt stress response. - Gene 191: 197-204, 1997. Go to original source...
    41. Nakashima, K., Jan, A., Todaka, D., Maruyama, K., Goto, S., Shinozaki, K., Yamaguchi-Shinozaki, K.: Comparative functional analysis of six drought-responsive promoters in transgenic rice. - Planta 239: 47-60, 2014. Go to original source...
    42. Ogbaga, C.C., Stepien, P., Johnson, G.N.: Sorghum (Sorghum bicolor) varieties adopt strongly contrasting strategies in response to drought. - Physiol. Plant. 152: 389-401, 2014. Go to original source...
    43. Potenza, C., Aleman, L., Sengupta-Gopalan, C.: Targeting transgene expression in research, agricultural, and environmental applications: promoters used in plant transformation. - In Vitro cell. dev. Biol. Plant. 40: 1-22, 2004. Go to original source...
    44. Placido, D.F., Campbell, M.T., Folsom, J.J., Cui, X., Kruger, G.R., Baenziger, P.S., Walia, H.: Introgression of novel traits from a wild wheat relative improves drought adaptation in wheat. - Plant Physiol. 161: 1806-19, 2013. Go to original source...
    45. Rohila, J.S., Jain, R.K., Wu, R.: Genetic improvement of Basmati rice for salt and drought tolerance by regulated expression of a barley Hva1 cDNA. - Plant Sci. 163: 525-532, 2002. Go to original source...
    46. Sanchez, P.L., Wing, R.A., Brar, D.S.: The wild relative of rice: genomes and genomics the genus Oryza: broadening the gene pool species into rice. - In: Zhang, Q., Wing, R.A. (ed.): Genetics and Genomics of Rice. Vol. 5. Pp. 9-25. Springer, New York 2014. Go to original source...
    47. Singh, B.P., Jayaswal, P.K., Singh, B., Singh, P.K., Kumar, V., Mishra, S., Singh, N., Panda, K., Singh, N.K.: Natural allelic diversity in OsDREB1F gene in the Indian wild rice germplasm led to ascertain its association with drought tolerance. - Plant Cell Rep. 34: 993-1004, 2015. Go to original source...
    48. Takahashi, R., Joshee, N., Kitagawa, Y.: Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice. - Plant mol. Biol., 26: 339-52, 1994. Go to original source...
    49. Tompa, P.: Intrinsically disordered proteins: a 10-year recap. - Trends Biochem. Sci. 37: 509-516, 2012. Go to original source...
    50. Tripathi, R., Boschetti, C., McGee, B., Tunnacliffe, A.: Trafficking of bdelloid rotifer late embryogenesis abundant proteins. - J. exp. Biol. 215: 2786-2794, 2012. Go to original source...
    51. Uga, Y., Sugimoto, K., Ogawa, S., Rane, J., Ishitani, M., Hara, N., Kitomi, Y., Inukai, Y., Ono, K., Kanno, N., Inoue, H., Takehisa, H., Motoyama, R., Nagamura, Y., Wu, J., Matsumoto, T., Takai, T., Okuno, K., Yano, M.: Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. - Nat. Genet. 45: 1097-1102, 2013. Go to original source...
    52. Wang, J., Oard, J.H.: Rice ubiquitin promoters: deletion analysis and potential usefulness in plant transformation systems. - Plant Cell Rep. 22: 129-134, 2003. Go to original source...
    53. Wang, M., Li, P., Li, C., Pan, Y., Jiang, X., Zhu, D., Zhao, Q., Yu, J.: SiLEA14, a novel atypical LEA protein, confers abiotic stress resistance in foxtail millet. - BMC Plant Biol. 14: 290-305, 2014. Go to original source...
    54. Wang, X.S., Zhu, H.B., Jin, G.L., Liu, H.L., Wu, W.R., Zhu, J.: Genome-scale identification and analysis of LEA genes in rice (Oryza sativa L.). - Plant Sci. 172: 414-420, 2007. Go to original source...
    55. Xiao, B., Huang, Y., Tang, N., Xiong, L.: Over-expression of a LEA gene in rice improves drought resistance under the field conditions. - Theor. appl. Genet. 115: 35-46, 2007. Go to original source...
    56. Xiao, F.H., Xue, G.P.: Analysis of the promoter activity of late embryogenesis abundant protein genes in barley seedlings under conditions of water deficit. - Plant Cell Rep. 20: 667-673, 2001. Go to original source...
    57. Yi, N., Oh, S.J., Kim, Y.S., Jang, H.J., Park, S.H., Jeong, J.S., Song, S.I., Choi, Y.D., Kim, J.K.: Analysis of the Wsi18, a stress-inducible promoter that is active in the whole grain of transgenic rice. - Transgenic Res. 20: 153-63, 2011. Go to original source...
    58. Yu, J.N., Zhang, J.S., Shan, L., Chen, S.Y.: Two new group 3 LEA genes of wheat and their functional analysis in yeast. - J. Integr. Plant Biol. 47: 1372-1381, 2005. Go to original source...
    59. Zhang, Z., Huang, R.: Analysis of malondialdehyde, chlorophyll, proline, soluble sugar, glutathione content in seedling. - Bio-protocol 3: e817, 2013. Go to original source...

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