biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 62:194-199, 2018 | DOI: 10.1007/s10535-017-0743-6

Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

K. Marciniak1,2,*, E. Wilmowicz1,2, A. Kuæko1, J. Kopcewicz1
1 Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Toruñ, Poland
2 Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruñ, Poland

Ent-kaurene oxidase (EKO) catalyze three sequential oxidations in the early steps of gibberellin biosynthesis pathway. In this research, a cDNA sequence of InEKO1 gene in the model short-day plant Ipomoea nil was identified. Our studies revealed that inductive conditions for flowering caused an increase in the transcriptional activity of the examined gene in the cotyledons-the main organs for the perception of the photoperiodic stimulus. In contrast, in the second half of the 16 h long inductive night and after that, a decreased amount of InEKO1 mRNA in the apexes was detected. What is more, ethylene, the key inhibitor of flower induction in I. nil, elevated the InEKO1 expression exclusively in the cotyledons between 10 and 14 h of the inductive night.

Keywords: ent-kaurene oxidase; flowering; phytohormones; short-day plant
Subjects: gene expression; flowering; gibberelins; ethylene

Received: December 19, 2016; Revised: March 15, 2017; Accepted: March 20, 2017; Published: January 1, 2018  Show citation

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Marciniak, K., Wilmowicz, E., Kuæko, A., & Kopcewicz, J. (2018). Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil. Biologia plantarum, 62(1), 194-199. doi: 10.1007/s10535-017-0743-6
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