biologia plantarum

Biologia plantarum 62:671-683, 2018 | DOI: 10.1007/s10535-018-0812-5

Transcriptomic analyses reveal genotype- and organ-specific molecular responses to cold stress in Elymus nutans

J.-J. Fu¹, J. C. Geng², Y.-J. Miao³, Y.-M. Xu³, T.-M. Hu^1,*, P.-Z. Yang^1,*

¹ Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China

² Shaanxi Grassland station, Xi'an, Shaanxi, China

³ College of Plant Science, Xi Zang Agriculture and Animal Husbandry College, Linzhi, Tibet, China

Elymus nutans is an important alpine perennial forage of the Pooideae subfamily, that can survive subzero temperatures. To understand the molecular mechanisms underlying cold tolerance in E. nutans, we performed the transcriptional analysis by RNA-Seq in two genotypes, the tolerant Damxung (DX) and the sensitive Gannan (GN), under cold stress. The new E. nutans transcriptomes comprised 200 520/200 836 and 181 331/211 973 transcripts in leaves/crowns of DX and GN, respectively. More cold-stress-related genes were identified in leaves than in crowns of both genotypes throughout the whole cold stress. The most prominent functional category in leaves of both genotypes at 3 h of stress was transcriptional regulation. Brassinosteroid and jasmonic acid mediated signalling pathways play central roles in regulating downstream protective responses in DX after 24 h of cold stress. Prolonged cold stress caused more severe transcriptome responses in crowns and leaves of DX compared to GN. The most significant transcriptomic changes in both genotypes were associated with the response to abiotic stresses and the oxidation-reduction processes, implying reprogramming of the cellular metabolism as an adaptation to cold stress. This study reveals mechanisms of genotype- and organ-specific cold stress response in E. nutans and thus provides a basis for future breeding strategies aimed at improving the tolerance of cold-sensitive plants.

Keywords: abiotic stress response; brassinosteroids; jasmonic acid; RNA-Seq

Subjects: transcription factors; RNA-seq analysis; cold stress; relative water content; malondialdehyde; proline; differentially expressed genes

Received: October 25, 2017; Revised: March 23, 2018; Accepted: March 28, 2018; Published: August 1, 2018  Show citation