biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 63:54-58, 2019 | DOI: 10.32615/bp.2019.007

Arabidopsis KIN gamma subunit 1 has a potential to regulate activity of sucrose nonfermenting 1-related protein kinase 2s (SnRK2s) in vitro

M. Punkkinen1, K. Denessiouk1,2, H. Fujii1,*
1 Molecular Plant Biology Unit, Department of Biochemistry, University of Turku, Turku, Finland
2 Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland

Plants must precisely regulate their signalling pathways to respond to environmental changes promptly. Sucrose non fermenting1 (SNF1)-related protein kinases (SnRK) 2 are essential kinases in abiotic stress responses, including responses to abscisic acid. Although homologs of SnRKs in yeast require a γ-subunit for full activation, it has been unclear whether SnRK2s in higher plants are affected by γ-subunits. In this report, we aimed to show the effect of Arabidopsis KIN γ-subunit 1 (KING1), which is a potential γ-subunit, on the activity of SnRK2. A recombinant KING1 bound to SnRK2.6 and functionally inhibited its activity in vitro. On the other hand, KING1 facilitated the activity of SnRK2.2. Structural models suggest that significant structural changes occurred as a result of KING1 binding to the C-terminal tail of SnRK2s. Since KING1 inhibited the kinase activity of a chimeric protein consisting of the N-terminal domain of SnRK2.6 and the C-terminal domain of SnRK2.2, regulation by KING1 was determined by the N-terminal domain of SnRK2s. Together, these results show that KING1 can mediate activity of SnRK2s in vitro.

Keywords: abscisic acid, activation of kinase, structural models.

Accepted: November 13, 2018; Prepublished online: November 14, 2018; Published online: January 19, 2019  Show citation

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Punkkinen, M., Denessiouk, K., & Fujii, H. (2019). Arabidopsis KIN gamma subunit 1 has a potential to regulate activity of sucrose nonfermenting 1-related protein kinase 2s (SnRK2s) in vitro. Biologia plantarum, 63, Article 54-58. https://doi.org/10.32615/bp.2019.007
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