biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:219-227, 2019 | DOI: 10.32615/bp.2019.025

Genome-wide analysis of heptahelical protein (HHP) gene family and expression of BcHHP1 in response to stresses in Brassica rapa

J. Wang1, F.Y. Huang1, X.L. Hou1,*, X. You2,*
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture, Nanjing 210095, P.R. China
2 College of Sciences, Nanjing Agricultural University, Nanjing 210095, P.R.China

Heptahelical protein (HHP) signalling pathway is involved in cold acclimation responses to low temperature and other stresses. The HHP transcription factor family is the key component regulating this signalling pathway. In this study, five HHP-like genes, BcHHP1, BcHHP2, BcHHP3, BcHHP4, and BcHHP5, were isolated from non-heading Chinese cabbage (Brassica rapa ssp. chinensis cv. Suzhouqing). Multiple sequence alignment and phylogenetic analysis showed that BcHHP proteins are highly homologous to HHP proteins from Arabidopsis thaliana, Glycine max, Oryza sativa, and Zea mays. Some of these HHP proteins might share similar functions in some aspects, which might be further proved by interaction network of BcHHP genes. Furthermore, real-time quantitative PCR showed that BcHHP1 was induced under cold and salt treatments. Besides, BcHHP1 was also accumulated in response to abscisic acid and salicylic acid, indicating that BcHHP1 gene might participate in response to hormone treatments. In addition, a BcHHP1-YFP fusion protein was localized to the nucleus and cytoplasm. These results indicated that five BcHHP genes might play important roles in a functional HHP signalling pathway responding to cold treatment. This work might be useful for future functional analysis of other HHP-like genes.

Keywords: abscisic acid, cold stress, expression analysis, non-heading Chinese cabbage, salicylic acid, salinity, subcellular localization

Accepted: November 15, 2018; Prepublished online: November 15, 2018; Published online: January 19, 2019  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wang, J., Huang, F.Y., Hou, X.L., & You, X. (2019). Genome-wide analysis of heptahelical protein (HHP) gene family and expression of BcHHP1 in response to stresses in Brassica rapa. Biologia plantarum, 63, Article 219-227. https://doi.org/10.32615/bp.2019.025
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileWANG5586Suppl.pdf

    File size: 853.48 kB

    References

    1. Chen, C.C., Liang, C.S., Ai-Ling, K., Yang, C.C.: HHP1 is involved in osmotic stress sensitivity in Arabidopsis. - J. exp. Bot. 60: 1589-1604, 2009. Go to original source...
    2. Chen, C.C., Liang, C.S., Kao, A.L., Yang, C.C.: HHP1, a novel signalling component in the cross-talk between the cold and osmotic signalling pathways in Arabidopsis. - J. exp. Bot. 61: 3305-3320, 2010. Go to original source...
    3. Chinnusamy, V., Jagendorf, A., Zhu, J.K.: Understanding and improving salt tolerance in plants crop. - Science 45: 437-448, 2005. Go to original source...
    4. Chinnusamy, V., Ohta, M., Kanrar, S., Lee, B.H., Hong, X., Agarwal, M., Zhu, J.K.: ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis. - Genes Dev. 17: 1043, 2003. Go to original source...
    5. Chinnusamy, V., Zhu, J., Zhu, J.K.: Cold stress regulation of gene expression in plants. - Trends Plant Sci. 12: 444, 2007. Go to original source...
    6. Gilmour, S.J., Zarka, D.G., Stockinger, E.J., Salazar, M.P., Houghton, J.M., Thomashow, M.F.: Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. - Plant J. cell. mol. Biol. 16: 433, 1998. Go to original source...
    7. Graham, A.D., Patterson, B.D.: Responses of plants to low, nonfreezing temperatures: proteins, metabolism, and acclimation. - Annu. Rev. Plant Physiol. Plant mol. Biol. 33: 347-372, 1982. Go to original source...
    8. Hsieh, M.H., Goodman, H.M.: A novel gene family in Arabidopsis encoding putative heptahelical transmembrane proteins homologous to human adiponectin receptors and progestin receptors. - J. exp. Bot. 56: 3137, 2005. Go to original source...
    9. Lee, B., Henderson, D.A., Zhu, J.K.: The Arabidopsis cold-responsive transcriptome and its regulation by ICE1. - Plant Cell 17: 3155-3175, 2005. Go to original source...
    10. Lee, H.G., Seo, P.J.: The MYB96-HHP module integrates cold and abscisic acid signaling to activate the CBF-COR pathway in Arabidopsis. - Plant J. cell. mol. Biol. 82: 962, 2015. Go to original source...
    11. Livak, K.J., Schmittgen, T.D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. - Methods 25: 402-408, 2001. Go to original source...
    12. Lyons, T.J., Villa, N.Y., Regalla, L.M., Kupchak, B.R., Vagstad, A., Eide, D.J., Palmiter, R.D.: Metalloregulation of yeast membrane steroid receptor homologs. - Proc. nat. Acad. Sci. USA 101: 5506-5511, 2004. Go to original source...
    13. Miura, K., Jin, J.B., Lee, J.: SIZ1- mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. - Plant Cell 19: 1403-1414, 2007. Go to original source...
    14. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. - Mol. Biol. Evol. 28: 2731-2739, 2014. Go to original source...
    15. Tang, J., Wang, F., Wang, Z., Huang, Z., Xiong, A., Hou, X.: Characterization and co-expression analysis of WRKY orthologs involved in responses to multiple abiotic stresses in Pak-choi (Brassica rapa ssp. chinensis). - BMC Plant Biol. 13: 1-13, 2013. Go to original source...
    16. Tang, Y.T., Hu, T., Arterburn, M., Boyle, B., Bright, J.M., Emtage, P.C., Funk, W.D.: PAQR proteins: a novel membrane receptor family defined by an ancient 7-transmembrane pass motif. - J. mol. Evol. 61: 372-380, 2005. Go to original source...
    17. Wei, Y., Hu, W., Xia, F., Zeng, H., Li, X., Yan, Y., He, C., Shi, H.: Heat shock transcription factors in banana: genome-wide characterization and expression profile analysis during development and stress response. - Sci. Rep. 6: 36864, 2016. Go to original source...
    18. Yamauchi, T., Kamon, J., Ito, Y.: Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. - Nature 423: 762, 2003. Go to original source...
    19. Yamaguchi-Shinozaki, K., Shinozaki, K.: Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. - Annu. Rev. Plant Biol. 57: 781-803, 2006.d Go to original source...
    20. Zhu, Y., Rice, C.D., Pang, Y., Pace, M., Thomas, P.: Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes. - Proc. nat. Acad. Sci. USA 100: 2231, 2003. Go to original source...

    Return to the content