biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:323-327, 2019 | DOI: 10.32615/bp.2019.037

Cerium nitrate improves salt tolerance of wheat seedlings by regulating the antioxidant capacity of chloroplasts

X.D. Chen1,2, C.J. Shan1,2,*
1 Henan Institute of Science and Technology, Xinxiang, 453003, P.R. China
2 Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang, 453003, P.R. China

The effects of Ce(NO3)3 on the antioxidant capacity in the chloroplasts of salt-stressed wheat (Triticum aestivum L.) seedlings were investigated. The results showed that salt stress (40, 80, 120, or 160 mM NaCl) significantly increased the activities of ascorbate peroxidase, glutathione reductase, superoxide dismutase, catalase, and glutathione peroxidase, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2), but significantly reduced the ratios of reduced ascorbate (AsA) to dehydroascorbate (DHA) and reduced glutathione to oxidizided glutathione (GSH/GSSG), the content of chlorophylls (Chls) and carotenoids, net photosynthetic rate, plant height, plant dry mass and root length compared with the control. Compared with NaCl stress alone, application of NaCl + Ce(NO3)3 further enhanced the activities of above mentioned enzymes, increased the ratios of AsA/DHA and GSH/GSSG, the content of Chs and carotenoids, net photosynthetic rate, plant height, plant dry mass, and root length, and significantly reduced the content of MDA and H2O2 in chloroplasts. Meanwhile, Ce(NO3)3 alone also significantly enhanced the above mentioned indicators, but had no significant effect on the content of MDA and H2O2 compared with the control. Our results suggested that Ce(NO3)3 up-regulated the antioxidant capacity of chloroplast, which improved the wheat salt tolerance.

Keywords: ascorbate peroxidase, carotenoids, catalase, chlorophylls, glutathione peroxidase, glutathione reductase, H2O2, malondialdehyde, net photosynthetic rate, Triticum aestivum

Accepted: January 9, 2019; Prepublished online: January 9, 2019; Published online: January 19, 2019  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Chen, X.D., & Shan, C.J. (2019). Cerium nitrate improves salt tolerance of wheat seedlings by regulating the antioxidant capacity of chloroplasts. Biologia plantarum, 63, Article 323-327. https://doi.org/10.32615/bp.2019.037
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    2. Brennan, T., Frenkel, C.: Involvement of hydrogen peroxide in the regulation of senescence in pear. - Plant Physiol. 59: 411-416, 1977. Go to original source...
    3. Dai, H., Shan, C., Jia, G., Yang, T., Wei, A., Zhao, H., Wu, S., Huo, K., Chen, W., Cao, X.: Responses to cadmium: tolerance, accumulation and translocation in Populus × canescens. - Water Air Soil Pollut. 224: 1504-1512, 2013. Go to original source...
    4. Fatehi, F., Hosseinzadeh, A., Alizadeh, H., Brimavandi, T., Struik, P.C.: The proteome response of salt-resistant and salt-sensitive barley genotypes to long-term salinity stress. - Mol. Biol. Rep. 39: 6387-6397, 2012. Go to original source...
    5. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases occurrence in higher plants. - Plant Physiol. 59: 309-314, 1977. Go to original source...
    6. Grace, S.C., Logan, B.A.: Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. - Plant Physiol. 112: 1631-1640, 1996. Go to original source...
    7. Griffith, O.W.: Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. - Anal. Biochem. 106: 207-212, 1980. Go to original source...
    8. Guo, Z., Yang, N., Zhu, C., Gan, L.: Exogenously applied poly-γ-glutamic acid alleviates salt stress in wheat seedlings by modulating ion balance and the antioxidant system. - Environ. Sci. Pollut. Res. - 24: 6592-6598, 2017. Go to original source...
    9. He, Z.Q., Zou, Z.R., He, C.X.: Effect of AMF on GSH-Px activity and cell membrane osmosis of tomato. - J. Northwest Sci-Tech. Univ. Agr. Forest. 34: 53-57, 2006.
    10. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. - Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
    11. Hodges, D.M., Andrews, C.J., Johnson, D.A., Hamilton, R.I.: Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. - Physiol. Plant. 98: 685-692, 1996. Go to original source...
    12. Hou, K., Bao, D., Shan, C.: Cerium improves the vase life of Lilium longiflorum cut flowers through ascorbate-glutathione cycle and osmoregulation in the petals. - Scientia Hort. 227: 142-145, 2018. Go to original source...
    13. Kato, M., Shimizu, S.: Chlorophyll metabolism in higher plants VII. Chlorophyll degradation in senescing tobacco leaves: phenolic-dependent peroxidative degradation. - Can. J. Bot. 65: 729-735, 1987. Go to original source...
    14. Lichtenthaler, H.K., Wellburn, A.L.: Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. - Biochem. Soc. Trans. 11: 591-593, 1983. Go to original source...
    15. Liu, R., Shan, C., Gao, Y., Wang, J., Xu, Z., Zhang, L., Ma, W., Tan, R.: Cerium improves the copper tolerance of turf grass Poa pratensis by affecting the regeneration and biosynthesis of ascorbate and glutathione in leaves. - Braz. J. Bot. 39: 779-785, 2016. Go to original source...
    16. Mao, C.X., Chen, M.M., Wang, L., Zou, H., Liang, C.J., Wang, L.H., Zhou, Q.: Protective effect of cerium ion against ultraviolet B radiation-induced water stress in soybean seedlings. - Biol. Trace Element Res. 146: 381-387, 2012. Go to original source...
    17. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. - Plant Cell Physiol. 22: 867-880, 1981.
    18. Ouyang, J., Wang, X.D., Zhao, B., Yuan, X.F., Wang, Y.C.: Effects of rare-earth elements on the growth of Cistanche deserticola cells and the production of phenylethanoid glycosides. - J. Biotechnol. 102: 129-134, 2003. Go to original source...
    19. Qu, C., Liu, C., Guo, F., Hu, C., Ze, Y., Li, C., Zhou, Q., Hong, F.: Improvement of cerium on photosynthesis of maize seedlings under a combination of potassium deficiency and salt stress. - Biol. Trace Element Res. 155: 104-113, 2013. Go to original source...
    20. Rossi, L., Zhang, W., Lombardini, L., Ma, X.: The impact of cerium oxide nanoparticles on the salt stress responses of Brassica napus L.. - Environ. Pollut. 219: 28-36, 2016. Go to original source...
    21. Ryu, H., Cho, Y.: Plant hormones in salt stress tolerance. - J. Plant Biol. 58: 147-155, 2015. Go to original source...
    22. Sorkheh, K., Shiran, B., Rouhi, V., Khodambashi, M., Sofo, A.: Salt stress induction of some key antioxidant enzymes and metabolites in eight Iranian wild almond species. - Acta Physiol. Plant. 34: 203-213, 2012. Go to original source...
    23. Wang, H., Feng, T., Peng, X., Yan, M., Tang, X.: Up-regulation of chloroplastic antioxidant capacity is involved in alleviation of nickel toxicity of Zea mays L. by exogenous salicylic acid. - Ecotoxicol. Environ. Safety 72: 1354-1362, 2009. Go to original source...
    24. Wu, M., Wang, P.Y., Sun, L.G., Zhang, J.J., Yu, J., Wang, Y.W., Chen, G.X.: Alleviation of cadmium toxicity by cerium in rice seedlings is related to improved photosynthesis, elevated antioxidant enzymes and decreased oxidative stress. - Plant Growth Regul. 74: 251-260, 2014. Go to original source...
    25. Xu, Q., Wang, Y., Liu, H., Cheng, J.: Physiological responses and chromosomal aberration in root tip cells of Allium sativum L. to cerium treatments. - Plant Soil 409: 447-458, 2016. Go to original source...
    26. Zheng, M., Guo, Y.: Cerium improves the vase life of Dianthus caryophyllus cut flower by regulating the ascorbate and glutathione metabolism. -Scientia Hort, 240: 492-495, 2018. Go to original source...

    Return to the content