biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:328-334, 2019 | DOI: 10.32615/bp.2019.038

Overexpression of the UGT76E12 gene modulates seed germination, growth, and response to NaCl, mannitol, and abscisic acid

L. Chen, W.-S. Wang, Q. Liu, R.-R. Dong, Y.-J. Li, T.-T Chen, B.-K. Hou*
The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education;
School of Life Science, Shandong University, Jinan 250100, P.R. China

Seed germination and following seedling growth are largely affected by environmental conditions. However, the genes involved in adaptations to these conditions are largely unknown. In this study, we cloned and characterized an Arabidopsis uridine diphosphate glycosyltransferase gene UGT76E12 and investigated its function in seed germination and plant growth under adverse environments. We found that UGT76E12 gene expression was induced by NaCl, mannitol, and abscisic acid (ABA) treatments. Under these treatments, the UGT76E12 overexpression lines exhibited a delayed seed germination and cotyledon growth compared with a wild type, and this delayed growth could be restored by treatment with sodium tungstate, an ABA synthesis inhibitor. Further, real-time quantitative PCR analysis reveals that the stress-induced expressions of genes involved in ABA biosynthesis were greatly enhanced in the UGT76E12 overexpression lines. Therefore, our data suggest that the UGT76E12 gene plays an important role in regulation of seed germination and growth under adverse environmental conditions by affecting ABA biosynthesis.

Keywords: adaptation, Arabidopsis thaliana, glycosyltransferase, growth inhibition, transgenic plants

Accepted: January 9, 2019; Prepublished online: January 9, 2019; Published online: January 19, 2019  Show citation

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Chen, L., Wang, W.-S., Liu, Q., Dong, R.-R., Li, Y.-J., Chen, T.-T., & Hou, B.-K. (2019). Overexpression of the UGT76E12 gene modulates seed germination, growth, and response to NaCl, mannitol, and abscisic acid. Biologia plantarum, 63, Article 328-334. https://doi.org/10.32615/bp.2019.038
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