biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 63:750-756, 2019 | DOI: 10.32615/bp.2019.108

Constitutive expression of the wheat TaSOD5 gene enhances salinity tolerance of Arabidopsis thaliana

Y.-G. SONG, T.-X. GAO, X.-J. LIU, W. DONG*
School of Life Science, Qufu Normal University, Qufu, Shandong, 273165, P.R. China

Superoxide dismutase is a crucial reactive oxygen species (ROS) scavenger and converts the superoxide radical (O2-) to H2O2, so it is thought to enhance abiotic stress tolerance by reducing ROS accumulation and so avoiding oxidative damage. In this study, we isolated a salt- and oxidative stress-responsive Cu/Zn SOD gene TaSOD5 from wheat. The ectopic overexpression of TaSOD5 in Arabidopsis increased total and Cu/Zn SOD activities, and offered the plant tolerance to salt stress. Arabidopsis ectopically expressing TaSOD5 possessed a superior resistance to oxidative stress induced H2O2. The TaSOD5 ectopic overexpression elevated the activities of both ROS scavengers and O2- producer NADPH oxidase. These findings show that Cu/Zn SOD enhances salt tolerance via regulating the machinery of redox homeostasis rather than improving SOD activity alone.

Keywords: APX, CAT, GPX, NADPH oxidase, redox homeostasis, ROS, superoxide dismutase, Triticum aestivum.

Received: March 19, 2019; Revised: July 27, 2019; Accepted: August 21, 2019; Published online: December 2, 2019  Show citation

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SONG, Y.-G., GAO, T.-X., LIU, X.-J., & DONG, W. (2019). Constitutive expression of the wheat TaSOD5 gene enhances salinity tolerance of Arabidopsis thaliana. Biologia plantarum, 63, Article 750-756. https://doi.org/10.32615/bp.2019.108
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