biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 64:32-42, 2020 | DOI: 10.32615/bp.2019.124

Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

Y.Q. HAN1,2, Y. SHI3, Y.M. GAO1, J.D. DU4, N.J. FENG4, Y.X. ZHANG4, D.F. ZHENG2,5,*
College of Life Science and Technology, Heilongjiang Bayi Agricultural University,
1 Daqing, Heilongjiang 163319, P.R. China
2 National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang 163319, P.R. China
3 Fu Dekang Economic and Trade Development Co., Beijing, 100031, P.R. China
4 College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China
5 College of Agriculture, Guangdong Ocean University, Zhanjiang, Guangdong 524088, P.R. China

Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.

Keywords: gene expression, lateral root, main root, RNA-seq, seed soaking, soybean.

Received: March 30, 2019; Revised: August 24, 2019; Accepted: September 30, 2019; Published online: January 13, 2020  Show citation

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HAN, Y.Q., SHI, Y., GAO, Y.M., DU, J.D., FENG, N.J., ZHANG, Y.X., & ZHENG, D.F. (2020). Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks. Biologia plantarum, 64, Article 32-42. https://doi.org/10.32615/bp.2019.124
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