biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 64:68-76, 2020 | DOI: 10.32615/bp.2019.047

Genes involved in strigolactone biosyntheses and their expression analyses in columnar apple and standard apple

X. SUN1,2, C. WEN1,2, H. HOU1,2, H. HUO3, J. ZHU1, H. DAI1,2, Y. ZHANG1,2,*
1 College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R. China
2 Qingdao Key Laboratory of Genetic Development and Breeding in Horticultural Plants, Qingdao Agricultural University, Qingdao 266109, P.R. China
3 Mid-Florida Research and Education Center, University of Florida, Apopka, FL 32703, USA

Columnar apple is a valuable resource for genetic improvement of cultivated apples due to its special tree architecture. Strigolactones (SLs) are a novel class of plant hormones controlling shoot branching. The content of SLs is higher in columnar apple than in standard apples. In this study, the members of major gene families involved in SLs biosynthesis and signaling were identified from apple genomic sequences and their expression profiles were characterized in columnar and standard apples using reverse transcription quantitative polymerase chain reactions. In comparison with standard apple, the higher expressions of MORE AXILLARY GROWTH genes MdMAX3-1 and MdMAX4-4 were detected in both buds and shoots of columnar apple but the expression of DWARF gene MdD53-4 showed a lower expression in columnar apple. Overexpression of Columnar gene MdCo31 in tobacco increased SLs content and weakened the inhibition of SLs signal transduction by increasing expression of MAX3 and down-regulating the transcription of D53. Thus MdCo31 could be a strong candidate gene for the control of columnar habit.

Keywords: Columnar gene; Malus × domestica, Nicotiana tabacum, shoot branching, tobacco.

Received: January 18, 2019; Revised: January 17, 2019; Accepted: January 28, 2019; Published online: January 29, 2020  Show citation

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SUN, X., WEN, C., HOU, H., HUO, H., ZHU, J., DAI, H., & ZHANG, Y. (2020). Genes involved in strigolactone biosyntheses and their expression analyses in columnar apple and standard apple. Biologia plantarum, 64, Article 68-76. https://doi.org/10.32615/bp.2019.047
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