biologia plantarum

Biologia plantarum 64:266-270, 2020 | DOI: 10.32615/bp.2019.152

Overexpression of a stilbene synthase gene from Fallopia multiflora causes trans-piceid accumulation in transgenic Arabidopsis

S. SHENG¹, W. XIA², W. RUI³, W. ZHAO², T. SHE¹, J. XIE¹, S. ZHAO^2,*, L. CHEN^4,*

¹ College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510225, P.R. China

² School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, P.R. China

³ Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 501006, P.R. China

⁴ Shenzhen Research Institute, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shenzhen 518057, P.R. China

Fallopia multiflora, also known as Polygonum multiflorum, is a traditional Chinese medicine with stilbenes as the main characteristic components. We previously isolated the presumed stilbene synthase gene FmSTS1 from F. multiflora and inserted the FmSTS1 gene into the genome of Arabidopsis thaliana under the control of cauliflower mosaic virus 35S promoter to evaluate its biological functions. Southern blot and Northern blot analyses were performed to confirm the integration and expression of FmSTS1 in Arabidopsis. A new stilbene compound, which was identified as trans-piceid, was found to accumulate in the transgenic Arabidopsis. This study firstly reportsFmSTS1 from F. multiflora that was heterologously expressed in transgenic Arabidopsis and resulted in the accumulation of trans-piceid, which was originally not synthesized in Arabidopsis species.

Keywords: CaMV promoter, HPLC detection, Northern blot, Southern blot.

Received: June 10, 2019; Revised: November 17, 2019; Accepted: December 5, 2019; Published online: March 20, 2020  Show citation