biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 64:271-279, 2020 | DOI: 10.32615/bp.2019.080

Salt tolerance of potato genetically engineered with the Atriplex canescens BADH gene

A. ALI1,2,3,*, Q. ALI3,4, M.S. IQBAL3, I.A. NASIR3, X. WANG2,*
1 Four Brothers Group Pakistan,77-D/1, 1st Floor Lahore Centre, Main Boulevard Gulberg, Lahore, Punjab 54000, Pakistan
2 Department of Plant Pathology, China Agricultural University, Beijing 100093, P.R. China
3 Centre of Excellence in Molecular Biology, University of Punjab, Lahore, Punjab 53700, Pakistan
4 Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Punjab 54000, Pakistan

Potato (Solanum tuberosum L.) is among the top staple foods in the world, and salinity adversely affects its yield and quality. To improve salt tolerance in potato, the present study is focused on the Agrobacterium-mediated transformation of potato by the Atriplex canescens betaine aldehyde dehydrogenase (BADH) gene driven by single, double, and triple CaMV 35S promoters. The study led first to the detection of seven lines containing the BADH gene followed by the identification of T-DNA insertions via DNA hybridization and enzyme-linked immunosorbent assays. The salt tolerance was found to be promoter dependent, as the lines with triple promoters showed a higher resistance than those tranformed with single and double promoters. The transgenic lines showed lower content of H2O2 and malondialdehyde and a lower relative electrical conductivity than wild-type plants. Furthermore, these lines also showed higher proline and chlorophyll content. In silico analysis confirmed that the A. canescens BADH protein had a remarkable tendency to interact with sodium ions and water molecules like other BADH proteins. Taken together, the overexpression of BADH under triple CaMV 35S promoters enhanced salt tolerance of potato.

Keywords: Atriplex canescens, CaMV 35S promoter, chlorophyll, malondialdehyde, proline, Solanum tuberosum.

Received: December 17, 2018; Revised: June 9, 2019; Accepted: June 11, 2019; Published online: March 27, 2020  Show citation

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ALI, A., ALI, Q., IQBAL, M.S., NASIR, I.A., & WANG, X. (2020). Salt tolerance of potato genetically engineered with the Atriplex canescens BADH gene. Biologia plantarum, 64, Article 271-279. https://doi.org/10.32615/bp.2019.080
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