biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 64:422-429, 2020 | DOI: 10.32615/bp.2020.046

Effects of exogenous nitric oxide and ethylenediaminetetraacetic acid on cadmium toxicity and accumulation in ryegrass

Q. ZHANG, Y. Y. ZHU, Y. J. DONG*
College of Resources and Environment, Shandong Agricultural University, Tai'an, 271018, P.R. China

The effects of exogenous nitric oxide (NO) and ethylenediaminetetraacetic acid (EDTA) on cadmium toxicity and accumulation in ryegrass (Lolium perenne L.) were studied in a hydroponic experiment. The results show that in plants without Cd application, addition of EDTA and sodium nitroprusside (SNP, an exogenous NO donor) significantly reduced the plant height, root length, and root activity of ryegrass, and significantly increased the O2*- generation rate and H2O2 and malondialdehyde (MDA) content in the aboveground and underground parts of ryegrass. Cadmium stress significantly inhibited ryegrass growth. Addition of SNP or EDTA alleviated Cd toxicity, and addition of both had a better effect. Compared with Cd alone, the shoot height and root length in the Cd+EDTA+SNP treatment increased by 68.8 and 59.6 %, and plant fresh and dry masses by 62.6 and 60.0 %, respectively. Also, the superoxide dismutase activity in the shoots and roots increased by 32.5 and 67.6 %, the peroxidase activity by 49.8 and 67.6 %, the ascorbate peroxidase activity by 134 and 102 %, the MDA content decreased by 30.4 and 21.8 %, and the O2*- generation rate by 29.0 and 26.1 %, respectively. At the same time, Cd content in the shoots and roots increased significantly by 89.7 and 30.2 %, respectively. Overall, the results suggest that exogenous NO could enhance Cd tolerance of ryegrass, but addition of EDTA could promote plant Cd uptake. Combined application of NO and EDTA increased Cd accumulation in the aboveground parts of ryegrass. In this experiment, the treatment of 100 µM CdCl2 + 0.25 mM EDTA + 50 μM SNP showed the best effects in promoting Cd accumulation in ryegrass and enhancing its Cd tolerance.

Keywords: ascorbate peroxidase, catalase, malondialdehyde, peroxidase, phytoremediation, sodium nitroprusside, superoxide dismutase.

Received: January 6, 2020; Revised: February 27, 2020; Accepted: March 11, 2020; Published online: May 29, 2020  Show citation

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ZHANG, Q., ZHU, Y.Y., & DONG, Y.J. (2020). Effects of exogenous nitric oxide and ethylenediaminetetraacetic acid on cadmium toxicity and accumulation in ryegrass. Biologia plantarum, 64, Article 422-429. https://doi.org/10.32615/bp.2020.046
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