biologia plantarum

Biologia plantarum 64:473-484, 2020 | DOI: 10.32615/bp.2020.066

Changes of cytosine methylation in pecan tissues of different stages by quantitative methylation-sensitive amplified polymorphism

Z.Z. LIU¹, F. ZHOU¹, J. SHANG¹, F.R. PENG^1,*, Z.H. MO², Y.R. LI³

¹ College of Forestry and Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R. China

² Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P.R. China

³ Green Universe Pecan Science and Technology Co., Nanjing 210007, P.R. China

Cytosine methylation plays an important role in plant development by regulating gene expressions. However, few studies have investigated methylation changes during the tissue differentiation and development of perennial plants. Here, the fluorescence-labeled methylation-sensitive amplified polymorphism method was used with eight primer combinations to detect methylation in leaves and xylem obtained at the stages of inflorescence emergence (IE), ovary start growth, and fruit maturity (FM) in two pecan (Carya illinoinensis) cvs. Pawnee and Stuart. The results show that the total methylation in the xylem was generally higher than in the leaves at each stage. Substantial methylation variations were observed at the amplified sites in pecan tissues at the various stages. The methylation patterns changed between the leaf and xylem, with frequencies from 44.97 to 67.01 % over the three stages in the two cultivars, among which the variation frequency between the tissues at the FM stage was the highest for each cultivar. The frequencies of methylation variation between the leaf samples at any two stages ranged from 31.86 to 45.88 %, with higher variation frequencies between the xylem samples (40.90 - 59.44 %) for each cultivar, which is consistent with the comparative results of polymorphism rates between the leaf and xylem over the three stages. Cluster analysis and principal coordinate analysis suggest that the xylem at the IE and FM stages had relatively distant epigenetic relationships with other tissue samples as a whole. This study reveals the patterns of methylation variation and methylation relationships among pecan tissues undergoing different developmental processes, implying the important roles of methylation in tissue differentiation and development of trees. These results lay a theoretical foundation for elucidating the regulatory mechanisms of methylation involved in tree development.

Keywords: Carya illinoinensis, fluorescence-labeled methylation-sensitive amplified polymorphism, plant development.

Received: October 5, 2019; Revised: December 13, 2019; Accepted: January 7, 2020; Published online: June 30, 2020  Show citation