biologia plantarum

Biologia plantarum 64:485-489, 2020 | DOI: 10.32615/bp.2020.051

Distribution of Na⁺ in roots and stem bases of buckwheat seedlings

W.-Y. ZHAN, Y.-C. YU, L.-X. HOU, C.-Y. LIU, F.-G. ZHAO, Y.-P. ZHANG, H.-B. YANG^*

Key Laboratory of Plant Biotechnology, University of Shandong Province,

College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, P.R. China

The localizations of sodium exclusion are roots and stem base, however, Na⁺ distribution in these localizations is unclear. Here, we used a salt-tolerant buckwheat cultivar Chuanqiao No.1 and a salt-sensitive cultivar TQ-0808 to demonstrate Na⁺ distribution. We found that Na⁺ content was highest in vacuole, the following was in cell wall or free space, and the least was in cytoplasm. Comparative analysis shows that Na⁺ accumulation in vacuole, cell wall, or free space of roots and stem base in 'Chuanqiao No.1' was obviously higher than in 'TQ-0808'; in contrast, Na⁺ accumulation in cytoplasm of 'Chuanqiao No.1' was less than in 'TQ-0808'. These results indicate that the capabilities of Na⁺ extrusion and Na⁺ compartmentalization of salt-tolerant buckwheat were obviously higher than of the salt-sensitive one, and the capabilities could effectively restrict Na⁺ transport to shoot. Compartmentalization of Na⁺ in the vacuole was the main way for Na⁺ exclusion of salt-tolerant buckwheat. In addition, the transcriptions of Na⁺/H⁺ antiporter 1 and salt overly sensitive 1 were remarkably higher in 'Chuanqiao No.1' than in 'TQ-0808', which is consistent with the above results.

Keywords: Fagopyrum esculentum, salt tolerance, TEM, transcription NHX1 and SOS1.

Received: December 26, 2019; Revised: March 16, 2020; Accepted: March 25, 2020; Published online: July 13, 2020  Show citation