biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 64:519-528, 2020 | DOI: 10.32615/bp.2020.075

Different responses of the halophyte Carex pumila to salt stress

S.W. WANG1,2, F.F. XU3, L.J. GUO3, T.T. HE1,2, X.L. LI5, L. YUAN1, Q.F. WANG1,4, H.T. LIU1,*
1 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, P.R. China
2 University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3 Collage of Life Sciences, Shangrao Normal University, Shangrao, Jiangxi 334001, P.R. China
4 Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei 430074, P.R. China
5 The Institute of Advanced Studies in Coastal Ecology, Ludong University, Yantai 264000, P.R. China

Salt stress is one of the most important stresses that affect coastal vegetation. A halophyte Carex pumila plays a crucial role in the maintenance of fragile ecosystems in coastal areas. Thus, understanding the mechanism of C. pumila responses to salt stress is a prerequisite for the conservation and utilization of this species. After treatment with 200, 300, 400, 500 mM NaCl for 60 h, C. pumila leaves displayed a decline in the relative water content and an increase in salt injury index. Osmolyte accumulation, as a function of osmotic adjustment, and antioxidant enzyme activities were maintained under salinity, even at a high NaCl concentration. High NaCl concentrations severely affected the photosystem II, the JIP-test indicated a significant decrease in performance indexes and quantum efficiencies and an increase in phenomenological fluxes. Metabolic analyses showed the changes of 39 metabolites, including 16 kinds of organic acids, 9 kinds of amino acids, 9 kinds of sugars, 3 kinds of sugar alcohols, and 2 amines. The identified metabolites were mainly involved in the glycolysis, pentose phosphate pathway, and tricarboxylic acids cycle.

Keywords: antioxidant enzymes, chlorophyll a fluorescence, metabolites, NaCl, osmotic adjustment.

Received: February 28, 2020; Revised: April 29, 2020; Accepted: May 18, 2020; Published online: July 29, 2020  Show citation

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WANG, S.W., XU, F.F., GUO, L.J., HE, T.T., LI, X.L., YUAN, L., WANG, Q.F., & LIU, H.T. (2020). Different responses of the halophyte Carex pumila to salt stress. Biologia plantarum, 64, Article 519-528. https://doi.org/10.32615/bp.2020.075
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