biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 64:807-813, 2020 | DOI: 10.32615/bp.2020.151

Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia

L. SANG1,2,3, L. PENG2, Z. QIU2, F. LUO1, G. CHEN1, L. GAO2, Y. YU2, J. LIU1,2,3,*
1 College of Horticulture, South China Agricultural University, Guangzhou 510642, P.R. China
2 College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
3 Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou, 510642, P.R. China

Ferredoxin-thioredoxin reductase (FTR) is an iron-sulfur protein that supplies electrons from photochemically reduced ferredoxin (Fd) to thioredoxin (Trx) in the ferredoxin/thioredoxin system in chloroplasts. The FTR is a heterodimer with a variable subunit (FTRv) and a catalytic subunit (FTRc). The function of FTRv is not well known. In petunia (Petunia hybrida), FTRv is a single-copy gene, which is named PhFTRv. In this study, the spatio-temporal expression of PhFTRv in petunia was analyzed, and PhFTRv transcription was found to be high in leaves and stems. A tobacco rattle virus gene silencing was used in this study. Virus induced gene silencing-mediated PhFTRv silencing resulted in large yellow-green leaves, delayed flowering, and reduced cold tolerance in petunia plants.

Keywords: flowering, chloroplasts, chlorophyll, leaf development, Petunia hybrida, photosynthesis, temperature stress.

Received: June 28, 2020; Revised: September 27, 2020; Accepted: October 12, 2020; Published online: December 15, 2020  Show citation

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SANG, L., PENG, L., QIU, Z., LUO, F., CHEN, G., GAO, L., YU, Y., & LIU, J. (2020). Virus induced PhFTRv gene silencing results in yellow-green leaves and reduced cold tolerance in petunia. Biologia plantarum, 64, Article 807-813. https://doi.org/10.32615/bp.2020.151
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