biologia plantarum

Biologia plantarum 65:118-125, 2021 | DOI: 10.32615/bp.2020.166

Rapid and efficient leaf regeneration propagation system for Euonymus bungeanus

K. NING¹, T. ZHOU², C.Z. JIANG¹, H.M. WU¹, J.L. JIANG¹, J. CHEN¹, Y.A. El-KASSABY^{3, *}, Y. MA^{1, *}

¹ College of Horticulture, Jinling Institute of Technology, Nanjing 210038, P.R. China

² Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P.R. China

³ Department of Forest and Conservation Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

Rapid propagation of plants by tissue culture is of great significance for large-scale production, molecular genetics research, and breeding. Currently, a rapid and high-efficient tissue culture protocol for Euonymus bungeanus is needed. To develop a propagation system for this species, we established a new regeneration system from leaves. Callus formation was induced on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm^-3 6-benzylaminopurine (6-BA) and 0.5 mg dm^-3 α-naphthalene acetic acid (NAA) and the induction rate almost reached 100 % under red radiation within 21 d. The medium for proliferation of adventitious buds comprised of MS medium with 1.0 mg dm^-3 6-BA and 0.5 mg dm^-3 NAA, and the induction rate within 20 d nearly reached 100 %. When, the adventitious buds were transferred to the rooting medium containing 1/2MS, 2.0 mg dm^-3 indole-3-butyric acid (IBA), and 0.05 mg dm^-3 NAA, adventitious root formation was achieved within 20 d. Collectively, the rapid and high-efficient regeneration system from E. bungeanus leaves was established, providing useful references for effective mass propagation and it could serve as an enabling technology for future genetic engineering.

Keywords: adventitious buds and roots, 6-benzylaminopurine, callus induction, indole-3-butyric acid, α-naphthalene acetic acid.

Received: June 5, 2020; Revised: November 16, 2020; Accepted: November 25, 2020; Published online: May 17, 2021  Show citation