biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 65:221-236, 2021 | DOI: 10.32615/bp.2021.010

The effect of Fusarium culmorum inoculation and deoxynivalenol application on proteome response in wheat cultivars Sumai 3 and SW Kadrilj

K. KOSOVÁ1, *, J. CHRPOVÁ1, J. ŠANTRŮČEK2, R. HYNEK2, M. KLÍMA1, I.T. PRÁŠIL1, P. VÍTÁMVÁS1
1 Division of Crop Genetics and Breeding, Crop Research Institute, Drnovská 507, 161 06 Prague 6, Czech Republic
2 Department of Biochemistry and Microbiology, University of Chemistry and Technology, Technická 3, 160 00 Prague 6, Czech Republic

Fusarium head blight (FHB) represents a damaging disease of cereal spikes caused by mycotoxin-producing Fusarium fungi revealing adverse effects on grain quality and yield. Chinese spring wheat (Triticum aestivum L.) cv. Sumai 3 represents a major source of FHB resistance thanks to Fhb1 QTL on 3BS chromosome conferring resistance to FHB and encoding a lectin-like protein. The aim of our study lied in a comparison of proteome response to Fusarium culmorum inoculation and mycotoxin deoxynivalenol (DON) application at 10 d after inoculation (dai) in spikes of resistant wheat cultivar Sumai 3 and susceptible wheat cultivar SW Kadrilj. Proteome analysis revealed profound impact of Fusarium inoculation and mycotoxin application on plant energy metabolism. Fusarium inoculation decreased photosynthesis and ATP biosynthesis and increased the level of stress-protective proteins (chaperones such as Hsc70, lectins). Genotype related differences observed at 10 dai indicated an active acclimation in Sumai 3 as indicated by increased content of some enzymes involved in phenolics biosynthesis (phenylalanine ammonia lyase PAL, BAHD acyltransferase), jasmonate biosynthesis (lipoxygenase LOX), and oligosaccharide biosynthesis (sucrose synthase SuSy, UDP-glucose uridylyltransferase) which are an important part of glycoproteins such as lectins and other pathogen-responsive biomolecules. The study thus provides data on plant acclimation to Fusarium infection which may underlie superior resistance of Sumai 3 cultivar.

Keywords: deoxynivalenol, energy metabolism, FHB resistance, Fusarium culmorum, protective proteins biosynthesis, proteomic analysis, stress-related proteins, Triticum aestivum.

Received: October 8, 2020; Revised: January 27, 2021; Accepted: February 12, 2021; Published online: July 30, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
KOSOVÁ, K., CHRPOVÁ, J., ŠANTRŮČEK, J., HYNEK, R., KLÍMA, M., PRÁŠIL, I.T., & VÍTÁMVÁS, P. (2021). The effect of Fusarium culmorum inoculation and deoxynivalenol application on proteome response in wheat cultivars Sumai 3 and SW Kadrilj. Biologia plantarum, 65, Article 221-236. https://doi.org/10.32615/bp.2021.010
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anderson, J.A., Stack R.W., Liu S., Waldron, B.L., Fjeld, A.D., Coyne, C., Moreno-Sevilla, B., Mitchell Fetch, J., Song, Q.J., Cregan, P.B., Frohberg, R.C.: DNA markers for Fusarium head blight resistance QTLs in two wheat populations. - Theor. appl. Genet. 102: 1164-1168, 2001. Go to original source...
    2. Bontpart, T., Cheynier, V., Ageorges, A., Terrier, N.: BAHD or SCPL acyltransferase? What a dilemma for acylation in the world of plant phenolic compounds. - New Phytol. 208: 695-707, 2015. Go to original source...
    3. Buerstmayr, H., Lemmens, M., Hartl, L., Doldi, L., Steiner, B., Stierschneider, M., Ruckenbauer, P.: Molecular mapping of QTL for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). - Theor. appl. Genet. 104: 84-91, 2002. Go to original source...
    4. Buerstmayr, H., Steiner, B., Hartl, L., Griesser, M., Angerer, N., Lengauer, D., Miedaner, T., Schneider, B., Lemmens, M.: Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. - Theor. appl. Genet. 107: 503-508, 2003. Go to original source...
    5. Caraux, G., Pinloche, S.: Permutmatrix: a graphical environment to arrange gene expression profiles in optimal linear order. - Bioinformatics 21: 1280-1281, 2005. Go to original source...
    6. Chambers, J.K., Macdonald, L.E., Sarau, H.M., Ames, R.S., Freeman, K., Foley, J.J. et al.: A G protein-coupled receptor for UDP-glucose. - J. biol. Chem. 275: 10767-10771, 2000. Go to original source...
    7. Chetouhi, C., Bonhommeee L., Lecomte P., Cambon F., Merlino M., Biron, D.G., Langin, T.: A proteomics survey on wheat susceptibility to Fusarium head blight during grain development. - Eur. J. Plant Pathol. 141: 407-418, 2015. Go to original source...
    8. Chivasa, S., Tomé, D., Slabas, A.: UDP-glucose pyrophosphorylase is a novel plant cell death regulator. - J. Proteome Res. 12: 1743-1753, 2013. Go to original source...
    9. Chrpová, J., Šíp, V., Štočková, L., Stemberková, L., Tvarůžek, L.: Resistance to Fusarium head blight in spring barley. - Czech J. Genet. Plant Breed. 47: 58-63, 2011. Go to original source...
    10. Cuthbert, P.A., Somers, D.J., Thomas, J., Cloutier, S., Brule-Babel, A.: Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat (Triticum aestivum L.). - Theor. appl. Genet. 112: 1465-1472, 2006. Go to original source...
    11. Ding, L.N., Xu, H.B., Yi, H.Y., Yang, L.M., Kong, Z.X., Zhang, L.X., Xue, S.L., Jia, H.Y., Ma, Z.Q.: Resistance to hemi-biotrophic F. graminearum infection is associated with coordinated and ordered expression of diverse defense signaling pathways. - PLoS ONE 6: e19008, 2011. Go to original source...
    12. Ding, L., Yang, R., Yang, G., Cao, J., Li, P., Zhou, Y.: Identification of putative phosphoproteins in wheat spikes induced by Fusarium graminearum. - Planta 243: 719-731, 2016. Go to original source...
    13. Ding, L., Li, M., Li, P., Cao, J.: Comparative proteomics analysis of young spikes of wheat in response to Fusarium graminearum infection. - Acta Physiol. Plant. 39: 271, 2017. Go to original source...
    14. Eggert, K., Pawelzik, E.: Proteome analysis of Fusarium head blight in grains of naked barley (Hordeum vulgare subsp. nudum). - Proteomics 11: 972-985, 2011. Go to original source...
    15. Eggert, K., Zörb, C., Mühling, K.H., Pawelzik, E.: Proteome analysis of Fusarium infection in emmer grains (Triticum dicoccum). - Plant Pathol. 60: 918-928, 2011. Go to original source...
    16. Glickman, M.H., Klinman, J.P.: Lipoxygenase reaction mechanism: demonstration that hydrogen abstraction from substrate precedes dioxygen binding during cartalytic turnover. - Biochemistry 35: 12882-12892, 1996. Go to original source...
    17. Gunnaiah, R., Kushalappa, A.C., Duggavathi, R., Fox, S., Somers, D.J.: Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat QTL (Fhb1) contributes to resistance against Fusarium graminearum. - PLoS ONE 7: e40695, 2012. Go to original source...
    18. He, Y., Mawhinney, T.P., Preuss M.L., Schroeder, A.C., Chen, B., Abraham, L., Jez, J.M., Chen, S.: A redox-active isopropylmalate dehydrogenase functions in the biosynthesis of glucosinolates and leucine in Arabidopsis. - Plant J. 60: 679-690, 2009. Go to original source...
    19. Janse van Rensburg. H.C., Van den Ende, W.: UDP-glucose: a potential signaling molecule in plants? - Front. Plant Sci. 8: 2230, 2018. Go to original source...
    20. Kijima, S.T., Staiger, C.J., Katoh, K., Nagasaki, A., Ito, K., Uyeda, T.Q.P.: Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells. - Sci. Rep. 8: 4381, 2018. Go to original source...
    21. Kosová, K., Chrpová, J., Šíp, V.: Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding. - Czech J. Genet. Plant Breed. 45: 87-105, 2009. Go to original source...
    22. Kosová, K., Chrpová, J., Šantrůček, J., Hynek, R., Štěrbová, L., Vítámvás, P., Bradová, J., Prášil, I.T.: The effect of Fusarium culmorum infection and deoxynivalenol (DON) application on proteome response in barley cultivars Chevron and Pedant. - J. Proteomics 169: 112-124, 2017. Go to original source...
    23. Kosová, K, Vítámvás, P., Urban, M.O., Prášil, I.T., Renaut, J.: Plant abiotic stress proteomics: the major factors determining alterations in cellular proteome. - Front. Plant Sci. 9: 122, 2018. Go to original source...
    24. Lannoo, N., Van Damme, E.J.M.: Lectin domains at the frontiers of plant defense. - Front. Plant Sci. 5: 397, 2014. Go to original source...
    25. Lemmens, M., Scholz, U., Berthiller, F., Dall´Asta, C., Koutnik, A., Schuhmacher, R., Adam, G., Buerstmayr, H., Mesetrhazy, A., Krska, R., Ruckenbauer, R.: The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium head blight resistance in wheat. - Mol. Plant-Microbe Interact. 18: 1318-1324, 2005. Go to original source...
    26. Leontovyčová, H., Kalachova, T., Trdá, L., Pospíchalová, R., Lamparová, L., Dobrev, P.I., Malínská, K., Burketová, L., Valentová, O., Janda, M.: Actin depolymerization is able to increase plant resistance against pathogens via activation of salicylic acid signalling pathway. - Sci. Rep. 9: 10397, 2019. Go to original source...
    27. Li, W.L., Faris, J.D., Muthukrishnan, S., Liu, D.J., Chen, P.D., Gill, B.S.: Isolation and characterization of novel cDNA clones of acidic chitinases and β-1,3-glucanases from wheat spikes infected by Fusarium graminearum. - Theor. appl. Genet. 102: 353-362, 2001. Go to original source...
    28. Lionetti, V., Giancaspro, A., Fabri, E., Giove, S.L., Reem, N., Zabotina, O.A., Blanco, A., Gadaleta, A., Bellincampi, D.: Cell wall traits as potential resources to improve resistance of durum wheat against Fusarium graminearum. - BMC Plant Biol. 15: 6, 2015. Go to original source...
    29. Liu, J.J., Li, L., Foroud, N.A., Gong, X., Li, C.C., Li, T.: Proteomics of bulked rachides combined with documented QTL uncovers genotype nonspecific players of the Fusarium head blight responses in wheat. - Phytopathology 109: 111-119, 2019. Go to original source...
    30. Maalekuu, K., Elkind, Y., Leikin-Frenkel, A., Lurie, S., Fallik, E.: The relationship between water loss, lipid content, membrane integrity and lox activity in ripe pepper fruit after storage. - Postharvest Biol. Technol. 42: 248-255, 2006. Go to original source...
    31. Makandar, R., Naiam, V., Chaturvedi, R., Jeannotte, R., Sparks, A.A., Shah, J.: Involvement of salicylate and jasmonate signaling pathways in Arabidopsis interaction with Fusarium graminearum. - Mol. Plant Microbe Interact. 23: 861-870, 2010. Go to original source...
    32. Mesterházy, A.: Types and components of resistance to Fusarium head blight of wheat. - Plant Breed. 114: 377-386, 1995. Go to original source...
    33. Mesterházy, A.: Theory and practice of the breeding for Fusarium head blight resistance in wheat. - J. appl. Genet. 43A: 289-302, 2002.
    34. Mesterházy, A., Tóth, B., Varga, M., Bartók, T., Szabó-Hevér, A., Farády, L., Lehoczki-Krsjak, S.: Role of fungicides, application of nozzle types, and the resistance level of wheat varieties in the control of Fusarium head blight and deoxynivalenol. - Toxins (Basel) 3: 1453-1483, 2011. Go to original source...
    35. Mugford, S.T., Qi, X., Bakht, S., Hill, L., Wegel, E., Hughes, R.K., Papadopoulou, K., Melton, R., Philo, M., Sainsbury, F., Lomonossoff G.P., Roy A.D., Goss R.J.M., Osbourn A.: A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats. - Plant Cell 21: 2473-2484, 2009. Go to original source...
    36. Noël, L.D., Cagna, G., Stuttmann, J., Wirthmüller, L., Betsuyaku, S., Witte, C.P., Bhat, R., Pochon, N., Colby, T., Parker, J.E.: Interaction between SGT1 and cytosolic/nuclear HSC70 chaperones regulates Arabidopsis immune responses. - Plant Cell 19: 4061-4076, 2007.
    37. Rawat, N., Pumphrey, M.O., Liu, S., Zhang, X., Tiwari, V.K., Ando, K., Trick, H.N., Bockus, W.W., Akhunov, E., Anderson, J.A., Gill, B.S.: Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight. - Nature Genet. 48: 1576-1580, 2016. Go to original source...
    38. Shahidi-Noghabi, S., Van Damme, E.J.M., Smagghe, G.: Carbohydrate-binding activity of the type-2 ribosome-inactivating protein SNA-I from elderberry (Sambucus nigra) is a determining factor for its insecticidal activity. - Phytochemistry 69: 2972-2978, 2008. Go to original source...
    39. Siedow, J.N.: Plant lipoxygenase: structure and function. - Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 145-188, 1991. Go to original source...
    40. Vančo, B., Šliková, S., Šudyová, V., Šrobárová, A.: Response to Fusarium culmorum inoculation in barley. - Biologia 62: 56-61, 2007. Go to original source...
    41. Waldron, B.L., Moreno-Sevilla, B., Anderson, J.A., Stack, R.W., Frohberg, R.C.: RFLP mapping of QTL for Fusarium head blight resistance in wheat. - Crop Sci. 39: 805-811, 1999. Go to original source...
    42. Wasternack, C., Hause, B.: Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. - Ann. Bot. 111: 1021-1058, 2013. Go to original source...
    43. Wang, Y., Yang, L.M., Xu, H.B., Li, Q.F., Ma, Z.Q., Chu, C.G.: Differential proteomic analysis of proteins in wheat spikes induced by Fusarium graminearum. - Proteomics 5: 4496-4503, 2005. Go to original source...
    44. Wang, W., Vignani, R., Scali, M., Cresti, M.: A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. - Electrophoresis 27: 2782-2786, 2006. Go to original source...
    45. Yang, F., Jensen, J.D., Svensson, B., Jørgensen, H.J.L., Collinge, D.B., Finnie, C.: Analysis of early events in the interaction between Fusarium graminearum and the susceptible barley (Hordeum vulgare) cultivar Scarlett. - Proteomics 10: 3748-3755, 2010a. Go to original source...
    46. Yang, F., Jensen, J.D., Spliid, N.H., Svensson, B., Jacobsen, S., Jørgensen, L.N., Jørgensen, H.J.L., Collinge D.B., Finnie C.: et al.: Investigation of the effect of nitrogen on severity of Fusarium Head Blight in barley. - J. Proteomics 73: 743-752, 2010b. Go to original source...
    47. Yang, F., Jacobsen, S., Jorgensen, H.J.L., Collinge, D.B., Svensson, B., Finnie, C.: Fusarium graminearum and its interactions with cereal heads: studies in the proteomics era. - Front. Plant Sci. 4: 37, 2013. Go to original source...
    48. Zhou, W., Eudes, F., Laroche, A.: Identification of differentially regulated proteins in response to a compatible interaction between the pathogen Fusarium graminearum and its host, Triticum aestivum. - Proteomics 6: 4599-4609, 2006. Go to original source...
    49. Zhou, W., Kolb, F.L., Riechers, D.E.: Identification of proteins induced or upregulated by Fusarium head blight infection in the spikes of hexaploid wheat (Triticum aestivum). - Genome 48: 770-780, 2005. Go to original source...

    Return to the content