biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 65:265-272, 2021 | DOI: 10.32615/bp.2021.025

Respiration responses of wheat seedlings to treatment with trehalose under heat stress

Y. LUO1, *, X.-Y. LIU1, Y.-J. XUE1, X.-Y. CAO2, J.-J. LIU2, M. GENG1
1 Equipment Sharing Platform of School of Life Sciences, East China Normal University, Shanghai 200241, P.R. China
2 Crop Research Institute, Shandong Academy of Agricultural Sciences/National Engineering Laboratory for Wheat and Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow and Huai River Valley, Ministry of Agriculture, Jinan 250100, P.R. China

Heat stress limits wheat production and trehalose can improve stress tolerance. How trehalose affects wheat respiration is unclear. In this study, we investigated the effects of exogenous trehalose on the respiration of wheat seedlings during heat stress and the subsequent recovery period. Trehalose pretreatment significantly increased the expression of the alternative oxidase genes AOX1a and AOX1c under heat stress, indicating that trehalose pretreatment increased the capacity of the alternative respiration pathway (AP) in response to heat stress. Trehalose pretreatment also enhanced the activity of the malate-oxaloacetate (Mal-OAA) shuttle and ameliorated the decrease in photosynthetic activity caused by heat stress. However, when the AP was inhibited by salicylhydroxamic acid under heat stress, both Mal-OAA shuttle activity and photosynthetic efficiency were substantially reduced in the control and trehalose pretreatment groups. In addition, trehalose pretreatment helped to maintain inner mitochondrial respiratory activity and the activity of Complex II during heat stress, particularly the coupling of oxidative phosphorylation with the Complex II electron transport chain, thereby mitigating heat-related damage to the cytochrome pathway (CP). Taken together, these results suggest that exogenous trehalose enhanced the AP and reduced damage to the CP under heat stress in wheat seedlings, thus maintaining cellular energy metabolism. Up-regulation of the AP by trehalose pretreatment may improve the heat tolerance of wheat seedlings by dissipating excess reducing equivalents transported through the Mal-OAA shuttle, thereby protecting photosynthetic performance.

Keywords: alternative respiration pathway, heat stress, malate-oxaloacetate shuttle, photosynthetic efficiency, trehalose, Triticum aestivum, wheat.

Received: December 16, 2020; Revised: April 26, 2021; Accepted: April 30, 2021; Published online: September 25, 2021  Show citation

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LUO, Y., LIU, X.-Y., XUE, Y.-J., CAO, X.-Y., LIU, J.-J., & GENG, M. (2021). Respiration responses of wheat seedlings to treatment with trehalose under heat stress. Biologia plantarum, 65, Article 265-272. https://doi.org/10.32615/bp.2021.025
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