biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 66:146-154, 2022 | DOI: 10.32615/bp.2021.073

Expression of genes encoding terpenoid biosynthesis enzymes during leaf development of Eucalyptus camaldulensis

N. ZHAN1, 2, L. HUANG3, Z. WANG4, J. ZHANG1, Y. XIE1, X. SHANG1, G. LIU1, Z. WU1, *
1 China Eucalypt Research Centre, Chinese Academy of Forestry, 524022, Guangdong, P.R. China
2 Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangdong 510640, P.R. China
3 Research Institute of Tropical Forestry, Chinese Academy of Forestry, 510520, Guangdong, P.R. China
4 Beihai City Protective Forest Farm, 536000, Guangxi, P.R. China

To reveal the regulation mechanism of terpenoid biosynthesis in the leaves of Eucalyptus camaldulensis, the content of volatiles in eucalyptus leaves and the transcriptome databases of young and mature leaves were analyzed. The results showed that E. camaldulensis contains 92 and 89 kinds of volatile substances in the young and mature leaves, respectively. Among them, the content of 1,8-cineole, β-pinene, and other substances was significantly different in young and mature leaves. A total of 99 802 unigenes were obtained from the transcriptome database of young and mature leaves of E. camaldulensis and 18 441 genes displayed obviously differential expressions during both developmental stages. There were 6 982 up-regulated unigenes and 11 461 down-regulated unigenes in the young leaf stage compared to the mature leaf stage. The key genes for terpenoid biosynthesis, including limonene synthase-10, limonene synthase-11, myrcene synthase-1, α- pinene synthase-2, and 1,8-cineole synthase-2, were selected for further analysis to explore the mechanism of gene regulation and genetic transformation. The expressions of key genes were validated by RT-qPCR, and their expressions were consistent with RNA-seq data. WRKY, MYB, NAC, and bHLH transcription factors (TFs) displayed important regulatory effects on the above key genes. Thus, a regulatory network model of terpenoid biosynthesis was constructed using target genes and TFs during leaf development in E. camaldulensis. These results provide theoretical evidence for understanding the terpenoid biosynthesis in plants and reference for terpenoids utilization by genetic engineering methods in E. camaldulensis.

Keywords: Eucalyptus camaldulensis, regulation mechanism, terpenoid biosynthesis, transcriptome, young and mature leaves.

Received: June 29, 2021; Revised: November 5, 2021; Accepted: November 24, 2021; Published online: May 26, 2022  Show citation

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ZHAN, N., HUANG, L., WANG, Z., ZHANG, J., XIE, Y., SHANG, X., LIU, G., & WU, Z. (2022). Expression of genes encoding terpenoid biosynthesis enzymes during leaf development of Eucalyptus camaldulensis . Biologia plantarum, 66, Article 146-154. https://doi.org/10.32615/bp.2021.073
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