biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 67:213-223, 2023 | DOI: 10.32615/bp.2023.022

Gene expression and biochemical profiling in the mitigation of heat stress in common bean using Bacillus subtilis

B.C. Lima1, T.R. Cruz2, A.F. RibaS1, T.B. Santos1, V. Cacefo1, F.F. Araujo1, *
1 Plant Production Post Graduate Program, Universidade do Oeste Paulista, CEP 19067-175, Presidente Prudente - SP, Brazil
2 Chemistry Post Graduate Program, Universidade Estadual Paulista "Júlio de Mesquita Filho" CEP 19060-900, Presidente Prudente - SP, Brazil

The present work aimed to evaluate the effect of heat stress on common bean (Phaseolus vulgaris L.) genotypes during the reproductive phase as a function of the inoculation of plants with Bacillus subtilis. The treatments were established by inoculating two strains of B. subtilis (AP-3 and AP-12) and a control. The plants were subjected to heat stress when they reached the reproductive stage, with an increase in temperature to 28/33°C. The duration of the stress period was ten days. Flowering, biochemical, and gene expression evaluations were performed. There was the interaction of B. subtilis AP-3 with the bean cultivar IAC-Imperador, reducing flower abortion, promoting the formation of new flower buds, and increasing the content of proline and guaiacol peroxidase activity in plant tissues. However, there was a reduction of transcription of genes encoding the 1-carboxylic acid-1aminocyclopropane oxidase and ethylene response factors and an increase of the Δ1-pyrroline-5-carboxylate synthetase1 gene. These results suggest that B. subtilis may modulate some metabolic pathways in response to high-temperature stress during the reproductive phase of the common bean. This also confirms that Bacillus strains represent a useful option to moderate abiotic stresses.

Keywords: gene expression, Phaseolus vulgaris L., plant growth-promoting rhizobacteria, stress tolerance.

Received: July 1, 2022; Revised: May 15, 2023; Accepted: May 16, 2023; Published online: August 23, 2023  Show citation

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Lima, B.C., Cruz, T.R., RibaS, A.F., Santos, T.B., Cacefo, V., & Araujo, F.F. (2023). Gene expression and biochemical profiling in the mitigation of heat stress in common bean using Bacillus subtilis. Biologia plantarum, 67, Article 213-223. https://doi.org/10.32615/bp.2023.022
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