Biologia plantarum 69:58-67, 2025 | DOI: 10.32615/bp.2025.006
Sulfur dioxide promotes wheat seed germination under drought stress and regulates antioxidant metabolism in wheat
- 1 School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, P.R. China
- 2 Hefei Fengle Seed Co., Ltd., Hefei 231283, P.R. China
- 3 Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, P.R. China
With the drastic changes in global climate and the increasing frequency of extreme weather events, abiotic stress poses a significant threat to future food crop production and is a major contributor to crop yield reduction (Ahmad et al., 2010). Wheat, one of the world's primary food crops, is susceptible to both abiotic and biotic stresses at all stages of its growth and development. Numerous studies have indicated that drought stress severely limits the growth and productivity of wheat (Xue et al., 2014; Faran et al., 2019). Therefore, understanding the effects of drought stress on wheat grain germination and developing strategies to mitigate the inhibition of germination caused by drought are crucial for enhancing wheat yield.
Keywords: antioxidants, drought stress, germination, sulfur dioxide, Triticum aestivum.
Received: March 9, 2025; Revised: July 8, 2025; Accepted: August 4, 2025; Published online: August 28, 2025 Show citation
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References
- Ahmad, P., Jaleel, C.A., Salem, M.A., Nabi, G. & Sharma, S. (2010) Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Critical Reviews in Biotechnology, 30, 161-175.
Go to original source... - Almaghrabi, O.A. (2012) Impact of drought stress on germination and seedling growth parameters of some wheat cultivars. Life Science Journal, 9, 590-598.
- Aroca, Á., Serna, A., Gotor, C. & Romero, L.C. (2015) S-sulfhydration: a cysteine posttranslational modification in plant systems. Plant Physiology, 168, 334-342.
Go to original source... - Boyer, J.S. (1970) Leaf enlargement and metabolic rates in corn, soybean, and sunflower at various leaf water potentials. Plant Physiology, 46, 233-235.
Go to original source... - Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
Go to original source... - Chan, K.X., Wirtz, M., Phua, S.Y., Estavillo, G.M. & Pogson, B.J. (2013) Balancing metabolites in drought: the sulfur assimilation conundrum. Trends in Plant Science, 18, 18-29.
Go to original source... - Choudhary, A.K., Singh, S., Khatri, N. & Gupta, R. (2022) Hydrogen sulphide: an emerging regulator of plant defence signalling. Plant Biology, 24, 532-539.
Go to original source... - Choudhury, F.K., Rivero, R.M., Blumwald, E. & Mittler, R. (2017) Reactive oxygen species, abiotic stress and stress combination. The Plant Journal, 90, 856-867.
Go to original source... - Chung, C.-Y., Chung, P.-L. & Liao, S.-W. (2011) Carbon fixation efficiency of plants influenced by sulfur dioxide. Environmental Monitoring and Assessment, 173, 701-707.
Go to original source... - Covarrubias, A.A., Ayala, J.W., Reyes, J.L., Hernandez, M. & Garciarrubio, A. (1995) Cell-wall proteins induced by water deficit in bean (Phaseolus vulgaris L.) seedlings. Plant Physiology, 107, 1119-1128.
Go to original source... - Dai, H., Xu, Y., Zhao, L. & Shan C. (2016) Alleviation of copper toxicity on chloroplast antioxidant capacity and photosystem II photochemistry of wheat by hydrogen sulfide. Brazilian Journal of Botany, 39, 787-793.
Go to original source... - Fàbregas, N. & Fernie, A.R. (2019) The metabolic response to drought. Journal of Experimental Botany, 70, 1077-1085.
Go to original source... - Faran, M., Farooq, M., Rehman, A. et al. (2019) High intrinsic seed Zn concentration improves abiotic stress tolerance in wheat. Plant and Soil, 437, 195-213.
Go to original source... - Finch-Savage, W.E., Leubner-Metzger, G. (2006) Seed dormancy and the control of germination. The New Phytologist, 171, 501-523.
Go to original source... - Han, Y., Yang, H., Wu, M. & Yi, H. (2019) Enhanced drought tolerance of foxtail millet seedlings by sulfur dioxide fumigation. Ecotoxicology and Environmental Safety, 178, 9-16.
Go to original source... - Hao, X., Li, W., Cao, H. et al. (2025) H2S promotes flowering in Brassica rapa ssp. pekinensis by persulfidation of the splicing factor BraATO2. Horticulture Research, uhaf190.
- Hu, K.-D., Bai G.-S., Li, W.-J. et al. (2015) Sulfur dioxide promotes germination and plays an antioxidant role in cadmium-stressed wheat seeds. Plant Growth Regulation, 75, 271-280.
Go to original source... - Hu, K.-D., Wang, Q., Hu, L.-Y. et al. (2014) Hydrogen sulfide prolongs postharvest storage of fresh-cut pears (Pyrus pyrifolia) by alleviation of oxidative damage and inhibition of fungal growth. PLoS ONE, 9, e85524.
Go to original source... - Hu, L.-Y., Hu, S.-L., Wu, J. et al. (2012) Hydrogen sulfide prolongs postharvest shelf life of strawberry and plays an antioxidative role in fruits. Journal of Agricultural and Food Chemistry, 60, 8684-8693.
Go to original source... - Janeček, ©. & ©evčík, J. (1999) The evolution of starch-binding domain. FEBS Letters, 456, 119-125.
Go to original source... - Jia, Y., Gu, X., Chai, J. et al. (2023) Rice OsANN9 enhances drought tolerance through modulating ROS scavenging systems. International Journal of Molecular Sciences, 24, 17495.
Go to original source... - Jin, Z., Sun, L., Yang, G. & Pei, Y. (2018) Hydrogen sulfide regulates energy production to delay leaf senescence induced by drought stress in Arabidopsis. Frontiers in Plant Science, 9, 1722.
Go to original source... - Khan, M.N., Khan, Z., Luo, T. et al. (2020) Seed priming with gibberellic acid and melatonin in rapeseed: Consequences for improving yield and seed quality under drought and non-stress conditions. Industrial Crops and Products, 156, 112850.
Go to original source... - Khan, M.N., Zhang, J., Luo, T. et al. (2019) Seed priming with melatonin coping drought stress in rapeseed by regulating reactive oxygen species detoxification: Antioxidant defense system, osmotic adjustment, stomatal traits and chloroplast ultrastructure perseveration. Industrial Crops and Products, 140, 111597.
Go to original source... - Kidokoro, S., Watanabe, K., Ohori, T. et al. (2015) Soybean DREB1/CBF-type transcription factors function in heat and drought as well as cold stress-responsive gene expression. The Plant Journal, 81, 505-518.
Go to original source... - Laisk, A., Pfanz, H. & Heber, U. (1988) Sulfur-dioxide fluxes into different cellular compartments of leaves photosynthesizing in a polluted atmosphere: II. Consequences of SO2 uptake as revealed by computer analysis. Planta, 173, 241-252.
Go to original source... - Landjeva, S., Neumann, K., Lohwasser, U. & Börner, A. (2008) Molecular mapping of genomic regions associated with wheat seedling growth under osmotic stress. Biologia Plantarum, 52, 259-266.
Go to original source... - Lei, K., Sun, S., Zhong, K. et al. (2021) Seed soaking with melatonin promotes seed germination under chromium stress via enhancing reserve mobilization and antioxidant metabolism in wheat. Ecotoxicology and Environmental Safety, 220, 112241.
Go to original source... - Li, L., Li, H., Wu, L. & Qi, H. (2022a) Sulfur dioxide improves drought tolerance through activating Ca2+ signaling pathways in wheat seedlings. Ecotoxicology, 31, 852-859.
Go to original source... - Li, L. & Yi, H. (2012) Differential expression of Arabidopsis defense-related genes in response to sulfur dioxide. Chemosphere, 87, 718-724.
Go to original source... - Li, Z.-G., Li, X.-E. & Chen, H.-Y. (2022b) Sulfur dioxide: an emerging signaling molecule in plants. Frontiers in Plant Science, 13, 891626.
Go to original source... - Lin, Y., Yi, X., Tang, S. et al. (2019) Dissection of phenotypic and genetic variation of drought-related traits in diverse Chinese wheat landraces. Plant Genome, 12, 190025.
Go to original source... - Liu, H., Wang, J., Liu, J., Liu, T. & Xue, S. (2021) Hydrogen sulfide (H2S) signaling in plant development and stress responses. aBIOTECH, 2, 32-63.
Go to original source... - Liu, H. & Xue, S. (2021) Interplay between hydrogen sulfide and other signaling molecules in the regulation of guard cell signaling and abiotic/biotic stress response. Plant Communications, 2, 100179.
Go to original source... - Liu, Y., Xu, H., Wen, X.-X. & Liao, Y.-C. (2016) Effect of polyamine on seed germination of wheat under drought stress is related to changes in hormones and carbohydrates. Journal of Integrative Agriculture, 15, 2759-2774.
Go to original source... - Luo, Z., Li, D., Du, R. & Mou, W. (2015) Hydrogen sulfide alleviates chilling injury of banana fruit by enhanced antioxidant system and proline content. Scientia Horticulturae, 183, 144-151.
Go to original source... - Mahpara, S., Zainab, A., Ullah, R. et al. (2022) The impact of PEG-induced drought stress on seed germination and seedling growth of different bread wheat (Triticum aestivum L.) genotypes. PLoS ONE, 17, e0262937.
Go to original source... - Maulana, F., Ayalew, H., Anderson, J.D., Kumssa T.T., Huang W. & Ma X.-F. (2018) Genome-wide association mapping of seedling heat tolerance in winter wheat. Frontiers in Plant Science, 9, 1272.
Go to original source... - Michel, B.E. & Kaufmann, M.R. (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51, 914-916.
Go to original source... - Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426-428.
Go to original source... - Mohamed, E.A., Ahmed, A.A.M., Schierenbeck, M. et al. (2023) Screening spring wheat genotypes for TaDreb-B1 and Fehw3 genes under severe drought stress at the germination stage using KASP technology. Genes, 14, 373.
Go to original source... - Moursi, Y.S., Thabet, S.G., Amro, A., Dawood, M.F.A., Baenziger, P.S. & Sallam, A. (2020) Detailed genetic analysis for identifying QTLs associated with drought tolerance at seed germination and seedling stages in barley. Plants, 9, 1425.
Go to original source... - Murillo-Amador, B., López-Aguilar, R., Kaya, C., Larrinaga-Mayoral, J. & Flores-Hernández, A. (2002) Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. Journal of Agronomy and Crop Science, 188, 235-247.
Go to original source... - Nonami, H. & Boyer, J.S. (1990) Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials. Plant Physiology, 93, 1610-1619.
Go to original source... - Ozturk, M., Turkyilmaz Unal, B., García-Caparrós, P., Khursheed, A., Gul, A. & Hasanuzzaman, M. (2021) Osmoregulation and its actions during the drought stress in plants. Physiologia Plantarum, 172, 1321-1335.
Go to original source... - Patel, M., Fatnani, D. & Parida, A.K. (2021) Silicon-induced mitigation of drought stress in peanut genotypes (Arachis hypogaea L.) through ion homeostasis, modulations of antioxidative defense system, and metabolic regulations. Plant Physiology and Biochemistry, 166, 290-313.
Go to original source... - Razi, K. & Muneer, S. (2021) Drought stress-induced physiological mechanisms, signaling pathways and molecular response of chloroplasts in common vegetable crops. Critical Reviews in Biotechnology, 41, 669-691.
Go to original source... - Riemenschneider, A., Wegele, R., Schmidt, A. & Papenbrock, J. (2005) Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana. The FEBS Journal, 272, 1291-1304.
Go to original source... - Sallam, A., Alqudah, A.M., Dawood, M.F.A., Baenziger, P.S. & Börner, A. (2019) Drought stress tolerance in wheat and barley: advances in physiology, breeding and genetics research. International Journal of Molecular Sciences, 20, 3137.
Go to original source... - Sheteiwy, M.S., Gong, D., Gao, Y., Pan, R., Hu, J. & Guan, Y. (2018) Priming with methyl jasmonate alleviates polyethylene glycol-induced osmotic stress in rice seeds by regulating the seed metabolic profile. Environmental and Experimental Botany, 153, 236-248.
Go to original source... - Siddiqui, M.H., Khan, M.N., Mukherjee, S. et al. (2021) Hydrogen sulfide (H2S) and potassium (K+) synergistically induce drought stress tolerance through regulation of H+-ATPase activity, sugar metabolism, and antioxidative defense in tomato seedlings. Plant Cell Reports, 40, 1543-1564.
Go to original source... - Surrey, K. (1964) Spectrophotometric method for determination of lipoxidase activity. Plant Physiology, 39, 65-70.
Go to original source... - Takahashi, H., Kopriva, S., Giordano, M., Saito, K. & Hell, R. (2011) Sulfur assimilation in photosynthetic organisms: molecular functions and regulations of transporters and assimilatory enzymes. Annual Review of Plant Biology, 62, 157-184.
Go to original source... - Wang, H., Moussa, M.G., Huang, W. et al. (2024) Exogenous hydrogen sulfide increased Nicotiana tabacum L. resistance against drought by the improved photosynthesis and antioxidant system. Scientific Reports, 14, 25534.
Go to original source... - Wang, W., Zhang, C., Zheng, W. et al. (2022) Seed priming with protein hydrolysate promotes seed germination via reserve mobilization, osmolyte accumulation and antioxidant systems under PEG-induced drought stress. Plant Cell Reports, 41, 2173-2186.
Go to original source... - Xue, Q., Rudd, J.C., Liu, S., Jessup, K.E., Devkota, R.N. & Mahan, J.R. (2014) Yield determination and water-use efficiency of wheat under water-limited conditions in the U.S. Southern High Plains. Crop Science, 54, 34-47.
Go to original source... - Yadav, V.K., Khan, N.A., Yadav, B. & Rathore, M.S. (2024) Comparative analysis of peroxidase and catalase isoenzymes via native-PAGE electrophoresis and SDS-PAGE profiling of leaf proteins in rice varieties under infection stress. Plant Cell Biotechnology and Molecular Biology, 25, 47-56.
Go to original source... - Yi, H., Liu, J. & Zheng, K. (2005) Effect of sulfur dioxide hydrates on cell cycle, sister chromatid exchange, and micronuclei in barley. Ecotoxicology and Environmental Safety, 62, 421-426.
Go to original source... - Yu, P., Li, S., Sun, Y. et al. (2024) Transcription factor VlbZIP14 inhibits postharvest grape berry abscission by directly activating VlCOMT and promoting lignin biosynthesis. International Journal of Molecular Sciences, 25, 9479.
Go to original source... - Zhang, H., Hu, S.-L., Zhang, Z.-J. et al. (2011) Hydrogen sulfide acts as a regulator of flower senescence in plants. Postharvest Biology and Technology, 60, 251-257.
Go to original source... - Zhao, T., Deng, X., Xiao, Q., Han, Y., Zhu, S. & Chen, J. (2020) IAA priming improves the germination and seedling growth in cotton (Gossypium hirsutum L.) via regulating the endogenous phytohormones and enhancing the sucrose metabolism. Industrial Crops and Products, 155, 112788.
Go to original source... - Zhu, D.-B., Hu, K.-D., Guo, X.-K. et al. (2015) Sulfur dioxide enhances endogenous hydrogen sulfide accumulation and alleviates oxidative stress induced by aluminum stress in germinating wheat seeds. Oxidative Medicine and Cellular Longevity, 2015, 612363.
Go to original source... - Zhu, J.-K. (2016) Abiotic stress signaling and responses in plants. Cell, 167, 313-324.
Go to original source...



