biologia plantarum

Biologia plantarum 69:77-87, 2025 | DOI: 10.32615/bp.2025.009

Efficient serological and molecular methods for the detection of tomato spotted wilt virus

Hana HOFFMEISTEROVÁ^{1, 2}, Emad IBRAHIM², Qinhai LIU³, Min ZHU³, Ladislav MENŠÍK⁴, Petr KOMÁREK⁵, Miroslav JURKA⁵, Xiaorong TAO³, Tomáš MORAVEC^{1, *}, Jiban KUMAR KUNDU^{1, 2, *}

¹ Laboratory of Virology-Centre for Plant Virus Research, Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 00 Prague, Czech Republic

² Plant Virus and Vector Interactions-Centre for Plant Virus Research, Czech Agrifood Research Center, Drnovská 507, 161 06 Prague, Czech Republic

³ Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China

⁴ Division of Crop Management Systems, Czech Agrifood Research Center, Drnovská 507, 161 06 Prague, Czech Republic

⁵ Hanácká zemědělská společnost Jevíčko a.s., Třebovská 713, 569 43 Jevíčko, Czech Republic

Tomato spotted wilt virus (TSWV; species Orthotospovirus tomatomaculae, family Tospoviridae) (Kuhn et al., 2023), is a negative strand RNA-virus containing envelope structures, which makes it unique among plant viruses (de Haan et al., 1991). TSWV ranks among the most destructive plant viruses worldwide. First described in Australia in 1919, TSWV has since attained a global distribution, infecting over 1 000 plant species across more than 85 families, including key agricultural crops such as tomato (Solanum lycopersicum), pepper (Capsicum annuum), groundnut (Arachis hypogaea), and various ornamentals (Parrella et al., 2003; Pappu et al., 2009). Infected plants typically exhibit chlorotic or necrotic spots, wilting, stunted growth, and in severe cases, complete crop failure, resulting in considerable economic losses, particularly in Solanaceous and Asteraceous crops (Roselló et al., 1996; Latham and Jones, 1998).

Keywords: dot blot, immune capture-reverse transcription PCR, one-enzyme reverse transcription-PCR, recombinase polymerase amplification, tissue blot immunoassay, tomato spotted wilt virus.

Received: October 2, 2025; Revised: October 24, 2025; Accepted: November 11, 2025; Published online: November 27, 2025  Show citation