Biologia plantarum, 1988 (vol. 30), issue 6
Article
Book reviews
Jana Pospíšilová, V. Škrdleta, F. Pospíšil
Biologia plantarum 30:478-480, 1988 | DOI: 10.1007/BF02890523 
Original Papers
Enucleation of protoplasts derived from suspension cultures of winged bean and from a crown gall cell line ofParthenocissus tricuspidata
H. Fakhrai, H. Haq, P. K. Evans
Biologia plantarum 30:401, 1988 | DOI: 10.1007/BF02890507 
Protoplasts derived from suspension cultures of the winged bean and a crown gall lineof Parthenocissus tricuspidata were enucleated by centrifugation on iso-osmotic discontinuous Percoli gradients and on discontinuous sucrose-mannitol density gradients. Enucleation was achieved according to the buoyant density of the protoplasts and preparations of both cytoplasts and miniprotoplasts were recovered. The Percoll gradients gave more satisfactory results than the sucrose-mannitol gradients. Enucleation was increased by raising the centrifugal force from 20 kg to 60 kg. The presence of nuclei was determined by the DNA specific probe 4'6-diamidino-2-phenylindole...
Nutritional changes induce xylogenesis in callus ofHaplopappus gracilis (NUTT.) A. Gray
Wiesława Kutternozińska, Lucyna Wajda, W. Korohoda
Biologia plantarum 30:409, 1988 | DOI: 10.1007/BF02890508 
Xylogenesis was induced in cultures of calli ofHaplopappus gracilis (NUTT.) A. Gray by metabolic shocks brought about by changes in nutrition. Xylogenesis was observed to occur in response to three changes in callus nutrition, caused by the following modifications of Eriksson's nutrient medium and culture conditions: i) omission of sucrose in the medium for 3 to 6 days and then transfer of the calli onto complete Eriksson's medium; ii) replacement of sucrose by 0.36*mol l-1 xylose for the whole culture period; iii) omission of nitrogen sources(i.e. glycine and NH4NO3 omission and replacement of KNO3...
Large scale micropropagation ofQuercus robur L. using adenine-type cytokinins and thidiazuron to stimulate shoot proliferation
V. Chalupa
Biologia plantarum 30:414-421, 1988 | DOI: 10.1007/BF02890509 
High rates of oak shoot multiplication were achieved on low salt nutrient media (BTM, WPM) supplemented with adenine type cytokinin or BAP. Low concentration of (0.2-0.6 mg 1-1) stimulated formation of a large number of axillary shoots and their elongation. Thidiazuron in very low concentration (0.001 - 0.002 mg 1-1) promoted shoot proliferation, in high concentration stimulated formation of large callus. More than twelve thousand micropropagated shoots were rooted in low salt agar media supplemented with low concentration of auxin (IBA or NAA 0.2 - 0.5 mg 1-1). High rooting percentages (81 %) were obtained. Survival...
Ethylene formation in the leaves of short- and long-day tobacco during transition to flowering
Veronika N. Lozhnikova, V. I. Rakitin, J. Krekule, Ivana Macháčková, M. K. Chailakhyan
Biologia plantarum 30:422, 1988 | DOI: 10.1007/BF02890510 
Ethylene formation by loaves of the central stem zone of the short-day tobacco cv. Maryland Mammoth and long-davNicntiana sylvestris was followed for 40 days during in duction and transition to flowering. In SD tobacco Mammoth, ethylene formation rose between days 0-10, remained unchanged for the next 10 davs, rose slightly between days 20 - 30 and sharply within the last 10 days. The time-course of ethylene formation by the leaves of LD tobacco N.silvestris resembled that of Mammoth, but tho changes were less pronounced, especially at the beginning of the period. Generally, ethylene formation is much higher in SD tobacco Mammoth than...
Relationships between nodulation and auxin level in pea roots
S. A. El-Desouky, V. Psota, J. Šebánek, G. N. Choi
Biologia plantarum 30:427, 1988 | DOI: 10.1007/BF02890512 
In the roots of 4-day-old pea plants germinated in unsterile soil from Rhizobium-inoculated seeds, a higher level of native IAA was determined than in roots of pea plants germinated in sterile soil from superficially sterilized seeds. The IAA level in plants grown from inoculated seeds increased markedly up to the age of 6 days of the plant, while in plants growing under sterile conditions it did not significantly change during the same period. Between the 6th and 10th days of the age of the plant, a decline in the IAA level was observed in roots of plante growing from inoculated seeds. It was not until after 10 days of age of the plant that the level...
Effect of pRi T-DNA transformation on growth and gas exchange of transplanted tobacco regenerants of the T and T' phenotypes
J. Václavík, Anna Konečná, M. Ondřej
Biologia plantarum 30:432-439, 1988 | DOI: 10.1007/BF02890513 
Five independent tobacco regenerant clones obtained after transformation withAgrobdcterium rhizogenes strain TR101 (four clones of the T phenotype, clone 5 a mixture of plants of the T and T' phenotypes) and untransformed plants cultivatedin vitro were gradually acclimated to the atmosphere and transferred to the soil substrate in pots. During 28 d after transplanting the increase of the leaf area was in T phenotype plants greater than in untransformed plants, but 14 d later no significant differences were observed between T phenotype and untransformed plants in leaf area and dry matter. T'phenotype plants, however, had significantly...
Sulphate ion influx and efflux inhydrodictyon reticulatum
Renata Rybová, Ludmila Nešpůrková, K. Janáček
Biologia plantarum 30:440, 1988 | DOI: 10.1007/BF02890514 
Sulphate ion is accumulated in the cells of the algaHydrodidyon reticulatum to a concentration approximately 30 mmol 1-1. The rate of SO4 2- uptake is increased markedly in the light, however, the effect is observable only after the cells have been kept in the light for about one hour. Uptake of sulphate is strongly inhibited by phosphorylation uncouplers and also by DIDS. The two-phase sulphate uptake kinetics, reported earlier was confirmed in experiments in which competition with phosphate anion was tested. Phosphate competes with sulphate only in the range of higher substrate concentrations (from 0.2 mmol...
Sulphate uptake by leaf mesophyll and bundle sheath cells of maize plants
C. Passera, M. Ferretti
Biologia plantarum 30:451, 1988 | DOI: 10.1007/BF02890515 
Uptake of35S-sulphate by bundle sheath strands (BSC) from leaves of maize plants (Zea mays L. ev. Dekalb L 72 A) was higher than that by isolated mesophyll protoplasts (MC) of maize. Ion uptake followed the Michaelis-Menten kinetic satuiation curves. SO2 4-uptake increased after addition of malate, NADPH, malate + NADP+ to BSC suspensions, but not to MC susp: nsions.
Effects of NaCl and Na2SO4 on Water Relations ofPlantago maritima (L.) andPlantago lanceolata (L.)
R. Houchi, A. Morant-Avice, G. Ferard, A. Coudret
Biologia plantarum 30:457, 1988 | DOI: 10.1007/BF02890516 
Transpiration and water absorption rates, stomatal and cuticular resistances to water vapour diffusion, were measured onPlantago maritima (halophyte) andPlantago lanceolata (glycophyte) grown in the presence of NaCl or Na2SO4. Water absorption was reduced in the presence of Na2SO4 and transpiration rate was increased when NaCl was added to the nutrient solution. The glycophyte daily water balance was more disturbed than that of the halophyte in the presence of Na2SO4.
Characterization of Albumins fromLupinus luteus L. Cotyledons
Ligia Konopska-Waliszkiewicz
Biologia plantarum 30:461, 1988 | DOI: 10.1007/BF02890517 
The cotyledons of yellow lupin seeds contain 15.4 mg of the albumins, water soluble proteins, per 100 mg of dry matter. These proteins were resolved by SDS gel electrophoresis into 13 polypeptides with relative molecular mass, Mr, between 16 000 and 84 000. Isoelectrofocusing analyses of the albumins showed that they were composed of six components with isoelectric points between 4.1 and 5.5. These results have indicated that the albumins are acid proteins and exhibited molecular and charge heterogeneity.
The effect of the glucosinolate sinigrin and of allyl isothiocyanate on the infectivity of turnip mosaic virus
J. Špak
Biologia plantarum 30:465, 1988 | DOI: 10.1007/BF02890518 
The effect of the glucoainolate sinigrin applied at concentrations from 5×10-6 to 5 × 10-3 g cm-3 on the infectivity of two isolates of turnip mosaic virus (TuMV) was studied. A statistically highly significant linear correlation relationship was found between the concentration of sinigrin added to the sap prepared from infectious plants ofPetunia hybrida hort. Vilm. cv. Lavina and the infectivity of the virus, expressed in the number of local necrotic lesions onNicotiana tabacum L. cv. Samsun leaves. Sinigrin caused a statistically significant decrease in the virus infectivity already at a concentration...
Brief Communications
ADP inhibition of the plasmalemma ATPase from Jerusalem artichoke tubers (Hèlianthus tuberosus L.)
G. Petel
Biologia plantarum 30:471, 1988 | DOI: 10.1007/BF02890519 
The inhibitory effect of ADP on the plasma membrane ATPase from the parenchyma cells of Jerusalem artichoke (Helianthiis tuberosus L.) tubers was investigated in both physiological states (dormant or non-dormant). To begin with, several sources of ADP were employed, as many commercial ADP contain a variable and non-negligible amount of vanadate the inhibitory effect of which on plasmalemma ATPase is well known. By using vanadium-free ADP, it was possible to show that the plasma membrane ATPase was inhibited by ADP. Similar levels of inhibition were measured in dormant and non-dormant samples, showing that endogenous ADP is not responsible for...
Leaf respiration: Possible cause of stimulation after low temperature treatment
N. P. Todaria
Biologia plantarum 30:475, 1988 | DOI: 10.1007/BF02890521 
Uncoupling of oxidative phosphorylation by 2,4 dinitrophenol stimulated leaf respiration rate measured as CO2 production or O2 uptake after low temperature treatment.
Book Review
Proceedings of the IAB Conference on Bryoecology, Part A + B. Symposia Biologica Hungarica 35
T. Herben
Biologia plantarum 30:426, 1988 | DOI: 10.1007/BF02890511 
On the economy of plant form and function. Proceedings of the Sixth Maria Moors Cabot Symposium, "Evolutionary Constraints on Primary Productivity: Adaptive Patterns of Energy Capture in Plants"
Javrmila Solárová
Biologia plantarum 30:474, 1988 | DOI: 10.1007/BF02890520 
Utilization, Treatment, and Disposal of Waste on Land. Proceedings of a workshop held in Chicago, IL, 6-7 Dec. 1985
Jarmila Solárová
Biologia plantarum 30:477, 1988 | DOI: 10.1007/BF02890522 


