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Determination of water deficit in disks cut out from leaf bladesJiøí ÈatskýBiologia plantarum 2:76, 1960 | DOI: 10.1007/BF02920701 The author describes a method for the determination of water deficit in disks punched out of leaves. The disks, 8 mm. in diameter, are placed on polyethylene rings and saturated in the apertures of a polyurethane foam plate which has been saturated with water (fig. 1, photo 1 and 2). This arrangement makes it possible for the tissue to be completely saturated in 3 hours with a minimum opportunity for infiltration. |
Chenopodium Quinoa Willd. as a local lesion host ofMarmor cichorii Kvíèala (Cichorium virus 1 Kvè.)Jaroslav BrèákBiologia plantarum 2:244-245, 1960 | DOI: 10.1007/BF02920654 Chicory blotch and yellows virus caused yellow local necrotic lesions on the leaves ofChenopodium Quinoa within nine days. Six strains of the common cucumber mosaic virus gave similar lesions in all cases, but in a shorter incubation period. |
The effect of gibberellic acid on the dynamics of chlorophyll synthesis in etiolated seedlingsZdenìk ©esták, Jaroslav UllmannBiologia plantarum 2:43, 1960 | DOI: 10.1007/BF02920696 Chlorophyll formation in etiolated seedlings of wheat and maize, grown for 8 days on Knop's nutrient solution with 10μg. gibberellic acid (further GA) added per ml. was investigated and compared with control plants. Under the light of fluorescent tubes of an intensity of 3000 lux a significant decrease in the amount of chlorophyll (a+b) was observed at all time intervals from 0 to 24 hr., expressed both per dry weight and per leaf length. Experimental results indicate a decreased rate of chlorophyll formation in the presence of GA. |
Zur Problematik der endogenen VirusbildungGerhard StaarBiologia plantarum 2:188-200, 1960 | DOI: 10.1007/BF02920648 Es wird über laufende Arbeiten berichtet.-Bei virösen Erkrankungen gehen dem Auftreten äusserlich erkennbarer Symptome cytologische Veränderungen voraus. Als solche werden u. a. charakteristische Zelleinschlüsse in jungen Keimen von Kartoffelknollen beschrieben. Mit ihrer Hilfe lässt sich nachweisen, dass viröse Prozesse auch in "gesund" getesteten Knollen vorkommen können.-Das unterschiedliche Auftreten spindelförmiger Zellkerne im Palisadenparenchym der Blätter von Solanum tuberosum wird als Zeichen verschieden stark ausgeprägter Neigung zu viröser Erkrankung (Virophilie) angesehen. Versuche zur chemisch-physikalischen Induktion viröser Prozesse ergaben ein bemerkenswertes Auftreten derartiger Kernformen in behandelten Pflanzen.-Die Virogenese wird als ein im Wesentlichen physiologischer Prozess aufgefasst, den die Pflanze gestaltet und der sich sowohl post infectionem als auch autonom entwickeln kann. Das anatomisch nachweisbare Vorkommen virogener Mikroprozesse und viröser Mikroherde macht den Nachweis, dass eine Pflanze virusfrei ist, unmöglich. Vielmehr ist mit der Ubiquität dieses Vorkommens zu rechnen. |
Problems of autosterility in Nicotiana alata Link et Otto (Formation of the inhibitory factor in the pistil)Jarmila TomkováBiologia plantarum 1:328-329, 1959 | DOI: 10.1007/BF02920852 From the partial results of artificial cultures in the presence of pistil tissue-pulp the author draws the preliminary conclusion that the inhibitory factor in the pistil ofNicotiana alataLink etOtto is already present before pollination. |
Studies on pectolytic enzyme system of Rhizoctonia solani Kühn properties of protopectinaseK. B. DeshpandeBiologia plantarum 2:139-151, 1960 | DOI: 10.1007/BF02920661 The study of physico-chemical properties of protopectinase secreted by R. solani was for the first time made and recorded in this paper. The results can be summarised as follows. |
The size of pollen grain of the genusTriticum L.Ivo CetlBiologia plantarum 2:287, 1960 | DOI: 10.1007/BF02920668 1. The aim of the present paper was an investigation of pollen grain size of different species of the Triticum L. natural polyploid series. |
Über die Bodenfeuchtigkeit, bei der die Pflanzen absterben®arko StojanovBiologia plantarum 2:79-87, 1960 | DOI: 10.1007/BF02920702 In der vorliegenden Arbeit ist eine einfache Methode beschrieben, mit deren Hilfe es möglich ist, die kritische Bodenfeuchtigkeit ganz objektiv zu bestimmen, bei der die Pflanzen absterben und zwar im Gegensatz zur Bestimmung des Welkungspunktes, die durch subjektive Fehler belastet ist. |
Staining of chromosomes in root tips of cloverin toto with aceto-gallocyanineJiøí Barto¹, Nadì¾da Avratov¹èukováBiologia plantarum 2:246, 1960 | DOI: 10.1007/BF02920655 |
Über die Wechselwirkung der Gibberellinsäure und anderer Stimulatoren in den WachstumskorrelationenRudolf DostálBiologia plantarum 2:48, 1960 | DOI: 10.1007/BF02920697 Weder die Dominanzverhältnisse zwischen den Sprossanlagen noch die Regulationswirkungen der Laub- und Keimblätter, die unter anderem für die vorzeitige Wachstumssistierung namentlich bei den Holzgewächsen verantwortlich sind, lassen sich durch Gibberellinsäure verändern. Doch setzt GS die von assimilierenden Blättern und vegetativen Speicherorganen ausgehenden Hemmungen herab und ersetzt in dieser Hinsicht die Stimulationswirkungen der Wurzeln, allerdings nur unvollständig, da es nachher zu den bekannten Ernährungs- und Gestaltungsstörungen kommt. Auf die Knollenbildung wirkt GS ungünstig, da die Terminalknospe der Knollenanlage zu starkem Streckungswachstum statt zu erforderlicher Ruhe gebracht wird. Die günstige Wirkung von GS auf die Blütenbildung bei der LangkurztagpflanzeBryophyllum crenatum tritt nur dann ein, wenn die mit GS behandelten Kurztagrosetten noch weiter im Kurztag gezogen werden. Somit kommt es hier mehr auf die phylogenetische Anpassung dieser Pflanzen an die Tropen, als auf gewisse stoffliche Einflüsse an, was mutatis mutandis auch von der Rhythmik der Bäume gilt. *** DIRECT SUPPORT *** A01GP005 00005 |
The seed progeny ofSolanaceae chimerasVladimír FuèíkBiologia plantarum 2:216-222, 1960 | DOI: 10.1007/BF02920650 A description is given of the production of two types of chimera between tomato and nightshade (Solanum koelreuterianum Winkler andS. geartnerianum Winkler). |
The influence of humus acid on the respiration of plant rootsMiroslava ©mídováBiologia plantarum 2:152-164, 1960 | DOI: 10.1007/BF02920662 Humus acid (Humussäure Riedel de Haen AG. Seelze Hannover) in 0.01% concentration increases the respiration intensity of plant roots grown in water cultures both as regards O2 consumption and CO2 production, while RQ (CO2:O2) is only very slightly increased. The plants used in these experiments were the winter wheat Py¹elka (Triticum vulgare Vill.), maize Zaj íèek's "Èeský koòský zub" (Zea mays L.) and the gourd Veltrusská velkoplodá (Cucurbita maxima L.). O2 consumption and CO2 production were determined on separated root tips by the direct Warburg method. It was found that the effect of humus acid is not only to increase respiration intensity in the roots of the experimental plants, but also their lengths and dry weights (for all experimental plants); for maize and gourd the dry weights of overground parts were also increased, which indicates that increased respiration intensity was linked with more intensive growth of the plants. |
Photosynthesis in leaf disks as a measure of photosynthetic capacity in crop plantsIvan ©etlík, Jiøí Barto¹, ©tìpán KubínBiologia plantarum 2:292, 1960 | DOI: 10.1007/BF02920669 In a previous communication (Barto¹, Kubín and©etlík 1960) a method was reported, which makes it possible to measure the average rate of photosynthesis in a representative sample of a large number of leaves. The sampling is made by cutting out disks of leaf tissue, following a definite pattern. Isolated leaf disks are then illuminated under controlled conditions and the rate of photosynthesis is estimated by weighing the increment of dry matter in a given time interval. |
Humic acid interactions in the growth processJiøí ØeøábekBiologia plantarum 2:88, 1960 | DOI: 10.1007/BF02920656 A characteristic feature of the effects of humic acids on plant growth is the stimulation of growth in length of the roots. This is sometimes explained as being due to the auxinoid nature of the acids. |
Changes in sunflower shoot structure under influence of gibberellic acidZ. ©esták, J. Èatský, J. SahulkaBiologia plantarum 2:247, 1960 | DOI: 10.1007/BF02920664 The spraying of 26-day-old sunflower plants with a solution containing 50 μg. gibberellic acid/ml. brought about morphological changes in leaves and a statistically significant increase in the leaf shape index (length: width of leaves). The number of leaves on the stem also increased, together with a rise in the value of the ratio of dry weight of stems to dry weight of leaves. |
Interspecific hybridization between Corchorus olitorius Linn. (strain C. G.) and C. sidoides F. Muell., and C. capsularis Linn. (strain D 154) and C. sidoides MuellR. M. Datta, S. K. DanaBiologia plantarum 2:61-66, 1960 | DOI: 10.1007/BF02920698 1. In C. olitorius ♀ × C. sidoides ♂, the pod set was 29.4 per cent and the percentage of pod harvested (out of the set pods) was 20 per cent. All harvested pods were empty. |
Respiratory pathways in gibberellin-treated wheat the effect of fluoride on the respiration rateJiøí Lu¹tinec, Jan Krekule, Vìra PokornáBiologia plantarum 2:223, 1960 | DOI: 10.1007/BF02920651 1. It was found that fluoride has a more pronounced inhibitory effect on the respiration of wheat leaves in plants grown in a solution of gibberellic acid than on that of control plants grown in water, during the entire development of the first leaf. |
The heritability of the amylolytic activity in barleyJosef NeèasBiologia plantarum 2:1-18, 1960 | DOI: 10.1007/BF02920693 During investigation of amylase activities as continuously variable biochemical features it was found that they are very sensitive to changes in the intensity of vegetative factors. The results of analysis of variance indicate that these characters not only tend to be inheritable themselves, but also the nature and intensity of their reactions to changes in the agro-ecological factors of the external environment show a similar tendency. The actual expression of these features in individuals belonging to a genetically homogeneous group must be considered as the resultant of all reactions of the realisation process concerned, on stimulatory and inhibitory influences, both internal and external. For the genetical analysis of hybrid groups it is necessary to have the most complete picture possible of this continuous modificability which masks the actual genetical variability. |
Aufklatschchromatographie der Zucker der durch Humussäure beeinflusten WeizenpflanzenAlena ÈinèerováBiologia plantarum 2:165-168, 1960 | DOI: 10.1007/BF02920663 Im vorliegenden Beitrag ist eine einfache Methode der Aufklatschchromatographie für die Bewertung des Zuckergehaltes in der Trockensubstanz der Aufklatsche (Aufklatschmasse) beschrieben. Diese Methode ist zum Zwecke der Verfolgung des Spiegels der einzelnen Zucker in den Wurzelspitzen des Weizens ausgearbeitet worden, der in Kulturen mit Humussäurezusatz bei verschiedener relativer Luftfeuchtigkeit wuchs. In der vorliegenden Arbeit ist er am Beispiel der Glukose, unter den Bedingungen niedriger, relativer Feuchtigkeit klargestellt worden. Der Glukosegehalt sinkt mit der Entwicklung der Pflanze bis zum Eintritt der Phase des zweiten Blattes. Während er in der Phase des ersten Blattes bei der Variante mit Humussäure grösser ist, ist dies in der Phase des zweiten Blattes bei den Kontrollpflanzen der Fall, |
Distribution of the humus substance fractions in plantsS. PrátBiologia plantarum 2:308-312, 1960 | DOI: 10.1007/BF02920670 The part of the radioactive fractions of humus substances which is soluble in alcohol (or in ether) spreads from drops of solution or from a mixture with lanoline paste mainly through the vascular bundles. The active component of humic acid evidently does not spread in the leaves, while the active component of fulvic acids fails to penetrate the tissues of some plants, but in others spreads to a distance of several centimetres. |
The Effect of β-Indolebutyric Acid on Phosphatase and Catalase Activity in Grape Vine CuttingsJiøí Lu¹tinecBiologia plantarum 1:99-106, 1959 | DOI: 10.1007/BF02927044 Phosphatase and catalase activity in the woody tissues of grape vine cuttings (Vitis vinifera), stimulated by β-indolebutyric acid, was studied at various temperatures. Its influence is evinced by increased root formation. The cause of the final effect is not, however, only quantitative acceleration of the metabolic processes. The parallelism of the activity changes of both enzymes in the stimulated and control tissues is evidence of this. |
A method of storage of leaf samples for chlorophyll analysisZdenìk ©estákBiologia plantarum 1:287, 1959 | DOI: 10.1007/BF02920848 In the course of experiments with leaf samples of pumpkin, sunflower, leafy fodder kale, sugar beet, tobacco, clover and begonia several, methods of storing material were compared with the object of determining the amount of chlorophyll (a+b) after 1 to 3 months. Chlorophyll analyses checked by paper chromatography provided evidence that the most reliable method of serial analyses is the following: Leaf discs are heated in very pure anhydrous acetone to its boiling point (with some MgCO3 present), rapidly closed with a paraffin stopper and then kept in the dark at temperatures just above 0°C. 50 leaf samples can thus be prepared for storage within 30 minutes. The decrease in total chlorophyll (a+b) content after 28 days of storage does not exceed 5%, after 84 days of storage 10% of the initial value. Chemical conversions of chlorophyll take place here which do not appreciably affect the spectrum of the acetone extract. The method cannot be applied to plants with acid-reacting cell juice (begonia, red varieties of fodder kale and the like). Other practicable methods of storage (a different storage in acetone, storage of fresh samples at +2 to +3°C, drying at +50°C, storage at laboratory temperature) bring about more serious destruction of the pigments. |
The relation of the refractive index of plant cell sap to its osmotic pressureBohdan SlavíkBiologia plantarum 1:48-53, 1959 | DOI: 10.1007/BF02927038 Parallel measurements of the osmotic pressure (cryoscopic method) and the refractive index of cell sap were carried out on 246 samples from seven plant species. At the same time both values were ascertained as well as their relation for aqueous solutions of some sugars, organic acids and inorganic salts. The relations found with these model solutions are different for different substances. The quantitative composition of osmotically active components of cell sap changes in the course of ontogenesis and as a result the relation of refractive index and osmotic pressure also changes. By this comparison it has been shown that a reliable and more or less linear relation of the two values, which is advantageous for practical application, is only valid for a limited period in the ontogenesis of the experimental plant. The determination of the osmotic pressure of cell sap from the refractive index, the main advantage of which is its quickness and the small volume of sap required (0-02 ml.), is reliable if we construct empiricallyad hoc the appropriate correlation graph for the experimental plant for a limited ontogenetic period. |
The application of extracted humus substances to overground parts of plantsZdenék SladkýBiologia plantarum 1:199-204, 1959 | DOI: 10.1007/BF02928685 The work presented in this paper was a study of the influence of different isolated humus substances when applied to the overground parts of plants by spraying on the leaves. The humus components employed showed varying effects on growth, respiration and chlorophyll content inBegonia semperflorens Link et Otto. The result of the physiological changes is reflected in morphological changes of the overground parts and particularly in the structure of the root system. Fulvic acids were found to be the most effective of the humus components when sprayed on leaves. |
Catalase activity in thermal blue-green algae in relation to temperature©tìpán KubinBiologia plantarum 1:3-8, 1959 | DOI: 10.1007/BF02927032 The author has studied the relation of the catalase activity in thermal blue-green algae to temperature. Experimental data were taken from the first phase of the reaction, so that oxidation of the enzyme by the substrate was eliminated. The relation of the activity of a homogenate of thermal blue-green algae to temperature gives a curve similar to that obtained bySizeR (1944) for pure enzyme preparations isolated from beef livers. The resistance of the enzyme to high temperatures is the same in thermal blue-green algae as in mesopholic seaweeds. The catalase of thermal blue-green algae does not exhibit any special characteristics differing from the catalase of mesophiles. |
Über den Einfluss äthanollöslicher organischer Kompoststoffe auf den Zuckermetabolismus in grünen PflanzenteilenVladimír TichýBiologia plantarum 1:157, 1959 | DOI: 10.1007/BF02927052 In der vorliegenden Arbeit ist der Einfluss der äthanolischen Fraktion des Bodenhumus auf das Gleichgewicht der Glycide in isolierten Blättern von Weizen (Triticum vulgare Vill.) Lindì (Tilia cordata Mill.) und Rosskastanie (Aesculus hippocastanum L.) studiert worden. |
Über den Einfluss einiger Humusstoffgruppen auf den Gehalt der oberirdischen Weizenteile an reduzierenden und nicht reduzierenden ZuekernVladimír TichýBiologia plantarum 1:107-112, 1959 | DOI: 10.1007/BF02927045 Der Einfluss der Alkohol-Benzen-Fraktion, der Fraktion der Huminsäuren sowie der Fraktion der Fulvosäuren, die in Konzentrationen von 0,1; 1; 10 und 100 mg je Liter wirkten, äussert sich im Gleichgewichte der Glycide in den oberirdisehen Teilen junger Weizenpflanzen folgendermassen: Die Alkohol-Benzen-Fraktion bewirkt ein Absinken des Gehaltes an nicht reduzierenden Zuekern (N) und ein Ansteigen des Gehaltes der reduzierenden Zucker (R), sodass der Quotient N: R herabgesetzt wird. Dies lässt sich als Anzeichen einer Erhöhung der Intensität katabolischer Vorgänge erklären. Die Fulvosäurenfraktion dagegen erhöht dieses Verhältnis, sodass sich auf eine Erhöhung der Intensität der Synthese schliessen lässt. Die Wirksamkeit der Huminsäurenfraktion ist wenig ausdrucksvoll. Die Beobachtungen dieser Veränderungen korrespondieren mit den Aenderungen des gesamten Trockengewichtes auf solche Weise, dass ein von der Fulvosäurenfraktion bewirkter hoher Quotient N: R mit einem Zuwachs des gesamten Trockengewichtes verbunden ist, während es sein durch die Alkohol-Benzen-Fraktion bewirkter niedriger Wert keineswegs ist. |
Peculiarities of the development of alternatives in relation to their winter-hardinessA. K. FedorovBiologia plantarum 1:295-301, 1959 | DOI: 10.1007/BF02920849 Some peculiarities of the development of wheat alternatives causing their winter-hardiness were investigated. |
A study of metabolism of exogenous tryptophane and β-indoleacetic acid in extirpated wheat embryosMilan Kutáèek, Kvìta Rokosová, Rudolf ØetovskýBiologia plantarum 1:54, 1959 | DOI: 10.1007/BF02927039 The metabolism of exogenous tryptophane (3. 10-2M) and β-indoleacetic acid (3. 10-2M) in extirpated wheat embryos was studied chromatographically and electrophoretically. |
Stimulation of the growth ofTrifolium pratense following exposure to low doses of chronic gamma irradiationRobert HonèarivBiologia plantarum 1:205-210, 1959 | DOI: 10.1007/BF02928686 Results of the action of low doses of gamma irradiation, intensity 1-80 to 0-24 r/day, are recorded in this paper. Irradiation was carried out on a gamma field with a 0-50 g. equiv. Ra Co60 source throughout the whole vegetative cycle.Trifolium pratense var. Jièínský was chosen as the experimental material. Growth and the yield of green matter was followed both on individually bedded out plants and on sown plots. Results of the measurement and weighing of the plants were statistically evaluated by means of the t-test and analysis of variation. Small, daily radiation doses (1.80-0.30 r per day) produced a significant stimulatory effect which was evidenced in the greater height of the irradiated plants as compared with the controls and a higher yield of green matter. In conclusion the paper deals with work that has already been carried out in this field. Experiments and measurements have shown that low doses of chronic gamma radiation are capable of stimulating plant growth. This work is confirmed by result obtained by Breslavets, Berezina and Shchibrya (1956) on other material. |


