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Mechanical rigidity and lignine content in different developmental phases of winter rape siliquaV. KratochvílBiologia plantarum 10:445-448, 1968 | DOI: 10.1007/BF02920987 The lignine content and mechanical rigidity of siliquae ofBrassica napus L. ssp.oleifera f.biennis, cv. Třebíčská were studied. Experimental plants were harvested in five consecutive periods and developmental stages of siliquae were determined on the basis of the weight of 1000 seeds. During the development of siliquae a gradual accumulation of lignine takes place; the content reached its maximum in certain developmental phases and then decreased. The mechanical rigidity of siliquae had a similar tendency. There were statistically significant differences in both properties studied within individual developmental phases. A highly significant positive correlation was found between lignine content and mechanical consistency. |
Effects of temperature on infection of French bean leaves (Phaseolus vulgaris L.) by lucerne mosaic virusJ. Bodnár, B. A. KvíčalaBiologia plantarum 10:251, 1968 | DOI: 10.1007/BF02921042 The effect of temperature on the number of lesions and the time of their appearance was studied by inoculating French bean leaves (Phaseolus vulgaris L. cv. Perlička) with lucerne mosaic virus either 24 or 48 h before or, 24 or 48 h after they were exposed to various temperatures. The temperatures tested were 23, 25, 27, 30, 33 and 36° C. |
Quantitative determination of cyanogenesis in plantsVáclav PokornýBiologia plantarum 5:310-317, 1963 | DOI: 10.1007/BF02930912 A method suitable for serial determinations in genetic experiments is described. Colorimetric determination of HCN (by 1-phenyl-3-methyl-5-pyra-zolone and pyridine, phenolphthalin or sodium picrate) facilitates processing of samples releasing 0.2 μg to 20 μg of HCN. Before hydrolysis of the glycoside the oil content in the seeds can be determined. Variance analysis of the cyanogenic properties of the particular seeds of two sorts of flax with an average HCN content of 0.4 and 0.9 μg shows that the differences between plants and between capsulae of the same plant can be determined. |
Development of the shoot apex ofChenopodium rubrum L. after photoperiodic induction in the cotyledon stageFrideta Seidlová, Jindra ŠtichováBiologia plantarum 10:131, 1968 | DOI: 10.1007/BF02921028 Development of the shoot apex up to floral differentiation was investigated in the short-day plantChenopodium rubrum. The changes occurring in the apex from energence until full opening of the cotyledons (Figs 1-4), development during photoperiodic induction (Figs. 5-8), as well as the resulting floral differentiation (Figs. 9-10) are described. It was aimed at excluding the influence of plastochron changes on the interpretation of ontogeny of the shoot apex. For that reason two planes of longitudinal sections and two plastochron stages were compared. |
Growth effects of 2-thiouracil and possibility of selective inhibition of floral differentiation inChenopodium rubrum L.Frideta Seidlová, J. KrekuleBiologia plantarum 10:41, 1968 | DOI: 10.1007/BF02920743 The effect of 2-thiouracil on vegetative growth and floral differentiation was investigated inChenopodium rubrum plants grown in water cultures. Between the low concentrations of the agent, stimulating vegetative growth and floral differentiation, and those inhibiting both these processes, a narrow concentration range was found (1.10-5 m to 2.10-5 m), where growth was inhibited selectively. At a concentration of 1.10-4 m a selective inhibition of development was found when 2-thiouracil was applied at the beginning of photoperiodic induction. Inhibition of development was strong regardless of whether 2-thiouracil was applied before, during or closely after 4 days of photoperiodic induction; the degree of growth inhibition, however, changed in dependence on photoperiodic induction. The strongest relative inhibition of development, calculated as a ratio between development and growth, was observed always at the beginning of photoperiodic induction. |
Changes in the level of endogenous gibberellins and auxins in apical buds ofChenopodium rubrum L. after application of growth substances reversing the effect of (2-chlorethyl)-trimethylammonium chloride (CCC) on floweringLola TeltscherováBiologia plantarum 10:305, 1968 | DOI: 10.1007/BF02921013 The application of CCC at concentrations inhibiting flowering ofChenopodium rubrum reduces the level of endogenous gibberellins in the apical buds of the plants. The effect of CCC may be reversed by appropriate concentrations of gibberellin (GA-), indole acetic acid (IAA) or kinetin. Kinetin applied to the apical bud during floral induction reduced the level of endogenous gibberellins similarly as CCC and if both CCC and kinetin were applied simultaneously their action was additive. On the other hand IAA applied under the same conditions increased the level of endogenous gibberellins and after joint application of CCC and IAA their level was the same as in untreated control plants. After application of CCC during floral induction the level of endogenous auxins did not change markedly but an active substance "x" appeared on the chromatograms of indole compounds. This substance was found also after simultaneous application of GA- and CCC but not after joint application of CCC and kinetin. If follows from our results that the same morphological phenomenon (flowering) can take place in plants considerably differing as to their level of endogenous growth substances. The ratio of different growth substances is obviously more important than the actual level of the single substances. |
Phenotypic, genotypic and environmental correlations of quantitative characters in winter wheatJ. SmočekBiologia plantarum 10:146-155, 1968 | DOI: 10.1007/BF02921031 In the experiment with 18 winter varieties the genotypic, phenotypic and environmental correlations were estimated in all combinations between the pairs of 11 quantitative characters. |
Comparative transmission of pea mosaic virus by one strain of Myzus persicae Sulz. and two strains of Acyrthosiphon pisum HarrisB. A. KvíčalaBiologia plantarum 10:51-57, 1968 | DOI: 10.1007/BF02920744 Experiments were conducted to compare the efficiency of transmission of a strain of pea mosaic virus (PMV) isolated in Czechoslovakia by two strains (clones) of the pea aphidAcyrthosiphon pisum Harris (green and red) and one strain ofMyzus persicae Sulz. PMV is a nonpersistent virus and the preliminary fasting of aphids before acquisition feeding increases the efficiency of aphids in transmission of this virus. In our experiments two hour fasted individuals were used and two periods of acquisition feeding on the source (1 and 5 minutes). On the healthy test plants the aphids were left over night. As the source and test plants pea (Pisum sativum L.) of the cv. Raman were used. During the one minute acquisition period on the source of infection the aphids were observed under the stereoscopic microscope. They usually made two to three probes. During the five minute acquisition feeding time the aphids were not observed and they were taken from the source of infection after a lapse of five minutes. To compare the efficiency in transmission of this virus by these aphids only one aphid per tested plant was used and in all trials only two to four day old nymphs were taken. Differences in transmission efficiency between two strains ofAcyrthosiphon pisum Harris were highly significant. The green strain of pea aphid was the less efficient vector in comparison with the green peach aphid and the red strain of the pea aphid, the latter being the most efficient vector of this virus. |
Phosphorus disturbances associated with potato leafroll virus infectionMarie Ulrychová, J. LimberkBiologia plantarum 10:311-317, 1968 | DOI: 10.1007/BF02921014 Potato leafroll virus induces considerable disturbances of phosphorus metabolism in leafroll-infected potato plants. Each variety reacts differently in this respect on the infection, to some extent being dependent on the susceptibility of the respective variety to leafroll and to the season as well. In varieties particularly susceptible to leafroll as in Apta and Sieglinde there is a decrease of total P after infection. On the contrary, varieties showing medium susceptibility such as varieties Tatranka, Rita and Ambra react to leafroll infection with a considerable increase of total P. In the comparatively less susceptible variety Krasava, there is no change in the level of total P after infection. In autumn, however, a slight decrease of total P even in the medium susceptible variety Tatranka, and the less susceptible variety, Krasava, can be found. Our results shed a new light on existing controversies in literature regarding phosphorus content in leafroll-infected potato plants. |
Effect of nutrition on the distribution of potassium and phosphorus inCucurbita pepo L.Olga VaňousováBiologia plantarum 10:157, 1968 | DOI: 10.1007/BF02921033 In young pumpkin plants, the phosphorus was found not to be affected substantially by potassium deficiency whereas the deficiency of calcium has marked effects. Potassium distribution is not affected substantially by either phosphorus or calcium deficiency. |
The effect of various microelements on changes in the activity of certain enzymes in hydroponically cultivated barley during the first period of growthJ. Pavel, Jiřina ŽákováBiologia plantarum 9:383, 1967 | DOI: 10.1007/BF02929782 The paper deals with the effect of the application of certain ions (Zn2+, B4O7 2- Mn2+, Cu2+, MoO4 2-,) on the development of the activity displayed by amylase, urease, and glutamate-oxalacetate (GOT) in hydroponically cultivated barley during the first seven days of ontogenesis. The enzymes were studied separately in the overground and root parts of the plants. The following main conclusions can be drawn from the findings made: The amylase activity of the control plants in connection with their development diminished slightly in the overground part, while it was relatively constant in the roots. Due to the effect of certain elements tested, this activity increased pronouncedly (significant differences were confirmed in variants containing MoO4 2-, Zn2+, and Co2+). The urease level in the overground part of the control plants remained low throughout the entire experimental period and was only little influenced by the individual microelements under study (with the exception of B4O7 2-). On the other hand, the urease activity in the root system increased markedly, the B4O7 2- ions exhibiting a significantly stimulating effect. The activity of glutamate-oxalacetate transaminase (GOT) in the overground part of the control plants increased only slightly in the course of the initial development, while in the root system it grew relatively more pronouncedly. In the overground part of the plants all the microelements administered displayed a significantly depressive effect on the development of this enzyme activity; in the roots, the inhibitory action was maintained only in the last experimental period in variants with Zn2+ ions. |
Types of the daily course of transpiration rate in seedlings of forest treesM. PenkaBiologia plantarum 9:407-415, 1967 | DOI: 10.1007/BF02926982 Types of the daily course of transpiration rate were studied in two-year old seedlings ofPinus silvestris L.,Picea excelsa L.,Larix decidua L.,Tilia cordata Mill., andAlnus glu-tinosa (L.) Gaertn. The curves illustrating the daily development of transpiration rate (Figs. 3 - 8) may be grouped in two types, showing a midday maximum (one-peak curves) and a midday depression (two-peak curves). Unlike herbaceous plants, the seedlings ofPinus silvestris L. were found to show, during the night-time, lower but not negligible values for the rate of transpiration (see Figs. 3 and 8). The following times are considered most suitable in determining the rate of transpiration for both herbs and woody species during the daylight: 09.0, 12.0, 14.0, and 17.0 hours. For the forest species seedlings further times are suitable for the night-time, as follows: 23.0, 01.0, and 06.0 hours. The rate of transpiration in the seedlings of Pinus silvestris attained very low levels at 3 and between 6 and 8 a.m. and between 6 and 8 p.m. (see Figs 3 and 8). |
B chromosome frequency in yugoslav rye populationsL. Zečević, Dobrila PaunovićBiologia plantarum 9:205-211, 1967 | DOI: 10.1007/BF02929739 The cytological analysis of 25 Yugoslav rye populations, collected from Slovenia Serbia and Macedonia, showed the presence of B chromosomes in the all populations analysed (100%). The number of B chromosomes present was small, from 1 to 3, while the average number per plant ranged from 0-13 to 0.97. The frequency of rye plants with B chromosomes for Yugoslavia had an average of 27.10%. The frequency of plants with B chromosomes and the average number of that chromosome category per plant were appreciably greater in the arid region of Macedonia than in the humid region of Slovenia, the differences being significant and real biometrically. |
Development and structure of foliage in wheat stands of different densityDanuše HodáňováBiologia plantarum 9:424-438, 1967 | DOI: 10.1007/BF02926984 The effect of sowing density (21, 85, 340 and 3400 plants per 1 m on the leaf area index (LAI), vertical arrangement of developing foliage and dry matter production was investigated in stands of wheat (Triticum aestivum L. cv. Niva). |
Water Uptake during Rapid Changes of Transpiration Induced by the Presence of High Concentration of Growth Substances in Root MediumV. KozinkaBiologia plantarum 9:222, 1967 | DOI: 10.1007/BF02929741 Immediate response of water uptake by intact plants ofPisum sativum L. to the introduction of high concentrations of α-naphtaleneacetic acid (NAA), β-indoleacetic acid (IAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), coumarin was determined by a series of potometric measurements. Growth substances were present in the root medium in two concentrations: 10-3 m and 10-2 m; the concentration of coumarin was 5. 10-4 m and that of EDTA was 10-3 m. |
The Effect of Wilting on the Redox Potential of Cereal LeavesJ. BenadaBiologia plantarum 9:447-453, 1967 | DOI: 10.1007/BF02926986 The redox potential of wilted leaves of cereal seedlings grown in a greenhouse was higher than in the fresh leaves. However, the redox potential in the wilted leaves of plants during the stem extension was lower than in fresh leaves. The reaction of barley leaves var. Japan 2003, which belongs to the dundar-beyi Zhuk.-group, differed materially from the European cereal varieties. The cause of the change of redox potential is discussed in connection with the ontogeny of cereals and resistance against obligate parasites. |
Peroxidases of different parts of the pumpkin plant (Cucurbita pepo L.)M. Dvořák, Jana ČernohobskáBiologia plantarum 9:308, 1967 | DOI: 10.1007/BF02929772 We compared the occurrence of peroxidase isozymes in protein extract from roots, hypocotyls and cotyledons of 10 dayCucurbita pepo plants and of adult leaves of older plants by means of starch gel and polyacryl amide gel electrophoresis. We reached maximum discrimination by means of starch gel electrophoresis: 11 zones were ascertained on the cathode side and about 2 on the anode side at pH 3.1. Two zones occurred regularly:A and (the latter having a more complicated structure). ZoneD is characteristic for roots, but is it suppressed and seldom found with leaves. On the other hand zonesC 1 andC 2 are clearly discernible with leaves but are substantially less evident with roots. The character of anodic zoneZ is discussed later in this paper. |
The relationship between sublethal water saturation deficit and the dynamics of soil moisture in some xerophilous grassesMilena RychkovskáBiologia plantarum 9:135, 1967 | DOI: 10.1007/BF02929730 The paper deals with the resaturation of water saturation deficit in fourStipa species tested under various ecological conditions. Attention is paid to the relations between the saturation and oversaturation types of curves, on the one hand, and soil moisture on the other. The oversaturation type appears in plants well supplied with soil moisture. The explanation of this phenomenon is discussed. |
Content of total chlorophyll and free amino acids of the chlorina mutant of arabidopsis thaliana in artificial glucose nutritionJiřina ŠvachulováBiologia plantarum 9:34, 1967 | DOI: 10.1007/BF02930733 When the lethal radiomutant chlorina 42 was cultivated on glucose containing and glucose-free medium the cotyledons of the matant sown on glucose medium had a higher chlorophyll content than those of the matant sown on glucose-free madium. In further cultivation of this mitant on glucose medium until flowering the total chlorophyll content is maintained at the same level, it increases slightly in the case of flowering plants. The mutant cultivated on glucose medium differs somewhat with regard to the content of free amino acids from the green control cultivated also on glucose medium over the whole vegetation period. The former has a higher glutamine content than the latter and in some growth phases also a higher content of asparagine. It is assumed that the insufficient photosynthetic activity is apparently not fully compensated by glucose and the plant has to supplement its energy balance by partial oxidative protein catabolism even under the conditions of artificial nutrition. |
The effect of carbon dioxide on the chondriom of some species of higher plantsMarie Naděžda KončalováBiologia plantarum 4:154-159, 1962 | DOI: 10.1007/BF02932937 The effect of C02 on the chondriome morphology in the meristematic tissue of pea, wheat and barley was observed. Results, evaluated on permanent preparations, are as follows: |
Growth response to different constant soil moisture levels in maize (Zea mays L.) during the vegetative phaseJ. VáclavíkBiologia plantarum 9:462, 1967 | DOI: 10.1007/BF02926988 The effect of different constant soil moisture levels (90, 60, 30% and 90, 60, 40% respectively, of the maximum capillary capacity) on the vegetative growth of maize was studied by the methods of growth analysis. The constant soil moisture in vegetation pots was maintained by means of the injection method. The constantly decreased soil moisture was applied in one experiment just from the sowing of germinated corns and in the other from the phase of 4-6 leaves. In both the cases plants responded to the different soil moisture levels by a progressively lower growth rate of the total dry weight according to the degree of soil moisture lowering. When the constant soil moistures were applied from the sowing, the differences in the relative growth rate (R.G.R.) were caused mainly by lower leaf area ratio (L.A.R.) values. In the case of constantly lowered soil moistures from the phase of 4-6 leaves, the differences in R.G.R. were caused by lower net assimilation rate (N.A.R.). When the constant soil moisture of 40% was applied from the sowing, the changes in N.A.R. showed the characteristic features of adaptation to unfavourable conditions. |
Impatiens parviflora DC. - a natural host of cabbage black ringspot and cucumber mosaic virusesZ. PolákBiologia plantarum 9:354, 1967 | DOI: 10.1007/BF02929777 Identification trials were carried out to determine a virus-like disease of Impatiene parviflora DC. Two forms of foliar symptoms were observed: |
The influence of light, darkness and changes in co2 and o2 concentration in die atmosphere on growth and gas exchange in pea (Pisum sativum)Jana Barthová, Sylva Leblová, J. KoštířBiologia plantarum 9:173, 1967 | DOI: 10.1007/BF02929735 When compared with the control plants, the increase in dry weight in the growing parts of germinating plants ofPisum sativum L., cultivated in a closed atmosphere in darkness or light is heavily inhibited. The concentration of carbon dioxide in the closed atmosphere increased in 60 mg CO2/per 1 g of the final dry weight of plants after 10 days. Similar results were obtained with older plants under similar conditions. The Knop solution caused a slight increase of dry weight in the growing parts of plants, but did not change considerably the relations in gas exchange. The results show that even plants which are able to photosynthetize, are only transpiring under the above conditions; first, when the plants were devoid of cotyledons, as storage organs, the changes in their dry weight (but not the changes in the CO2 and O2 exchange in a closed atmosphere) seemed to show photosynthetic income of CO2 (while its concentration increased to 3-6 volume %) when compared with the control. |
Metabolic disturbances in nicotiana glauca, a symptomlessMarie Ulrychová, Jaroslav LimberkBiologia plantarum 9:56, 1967 | DOI: 10.1007/BF02930736 Metabolic disturbances inNicotiana glauca, a symptomless carrier of potato witches' broom were studied. The dry weight content of leaves of diseased plants was slightly decreased, that of the stems increased. The ash content in the dry weight of the whole diseased plant was decreased by 11%. Contrary to tomato plants which had shown considerable disproportions in the distribution of metabolites among the individual organs investigated (ULRYCHOVÁ, LIMBERK 1964), the content of the total nitrogen and of the individual nitrogen fractions of both leaves and stems of diseasedNicotiana glauca were decreased. The content of glutamine was increased in both organs in spite of the decrease of ammonia nitrogen by nearly 30% in both cases. The most striking disturbance found in the diseased plants ofNicotiana glauca was the 67% increase of a low-molecular phosphorus fraction (P extractable with 0-2N HCIO4) in the leaves. This result agrees with that found in diseased tomato plants and may be primarily associated with the virus infection. The content of all other phosphorus fractions of both leaves and stems was decreased. |
Increased sensitivity of the respiratory system of plants grown in gibberellic acid toward fluorideJiří Luštinec, Věra Pokorná, Jiří RůžičkaBiologia plantarum 4:119, 1962 | DOI: 10.1007/BF02932932 1. It was found in agreement with the results of Fang and co-workers (1960) that gibberellic acid in concentrations between 2 and 80 mg./l. does not affect the ratio of radioactivities of14CO2 liberated from glucose-6-14C and -1-14G (C6/C1) when acting for several hours on sliced wheat leaves, and that at a concentration of 10 mg./l. it does not alter the degree of inhibition of respiration due to fluoride, iodoacetate and malonate or the consumption of oxygen. A linear relationship was established between the decrease in14CO2 liberation from glucose-14C and the concentration of gibberellic acid. |
Growth of radish (Raphanus sativus) seedlings in magnetic fieldsM. H. SimmersBiologia plantarum 9:377-382, 1967 | DOI: 10.1007/BF02929781 Radish seedlings were grown in asymmetric magnetic fields. The number of seeds germinated and the dry weight of the plants were the two criteria by which possible effects were examined. Two experiments were done; in the first the plants were grown for an average of 7.7 days and in the second for 14.1 days. A statistical analysis of the results failed to reveal any significant difference between control plants grown in dummy magnets and those subjected to the magnetic influence. |
Redox potential gradients in the flowerJ. BenadaBiologia plantarum 9:202-204, 1967 | DOI: 10.1007/BF02929738 Distinct redox gradients were found in flowers and stalks of some plants. The ovary was found to be the most negative part in the whole pistil and more negative than the coloured perigon or corona leaves. These redox differences are in accordance with biopotential differences obtained in flowers by other authors. |
The toxic effect of the cultivation medium of plants infected with tobacco mosaic virusVěra SosnováBiologia plantarum 7:472-473, 1965 | DOI: 10.1007/BF02920692 |
Spiral feedback oscillations of growing hypocotyl with radicle inPisum sativum L.M. SpurnýBiologia plantarum 8:381, 1966 | DOI: 10.1007/BF02930674 Cinematographic records of longitudinal growth showed that hypocotyl with radicle inPisum sativum L. undergoes spiral oscillations during growth. This phenomenon can be characterized by the following time-space limits: |
The effect of zinc on the biosynthesis of tryptophan, andol auxins and gibberellins in barleyNikola Mašev, Milan KutáčekBiologia plantarum 8:142, 1966 | DOI: 10.1007/BF02930623 The action of zinc on the growth of barley and the biosynthesis of indol compounds and gibberellin-like substances was investigated in a number of concentrations of zinc from doses stimulating growth to toxic doses. The seeds were soaked before sowing in solutions of zinc sulphate (5.10-5 to 5.10-1% Zn), and the plants cultivated for 7 days in water. Lower concentrations of zinc increased both plant growth and the biosynthesis of tryptophan and auxins. At the optimum concentration of 5.10-3% Zn this increase in tryptophan amounted to 241% of the variant without zinc; in substances with an RF corresponding to indolyacetic acid, the increase determined by the biological test, was 207% as against the variant without zinc. Higher concentrations of zinc inhibited growth, the tryptophan content was decreased to below that of the control without zinc and the auxin content also fell to below the control values. Zinc also influenced the content of gibberellin-like substances in the plants. At a concentration of 5.10-3% Zn the increase in the growth activity in the gibberellic acid area of the chromatogram was 294% of the variant without zinc. At toxic concentrations of zinc, the content of gibberellin-like substances fell to below that of the controls. The finding that zinc acts simultaneously on the biosynthesis of auxins and gibberellins is also evidence for the common action of growth substances of various chemical types on plant growth. |


