biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:417-421, 2001 | DOI: 10.1023/A:1012463315579

An Antifungal Protein Purified from Pearl Millet Seeds Shows Sequence Homology to Lipid Transfer Proteins

R. Velazhahan1, R. Radhajeyalakshmi1, R. Thangavelu1, S. Muthukrishnan2
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore-, India
2 Department of Biochemistry, Kansas State University, Manhattan, USA

In the course of a search for antifungal proteins from plant seeds, we observed inhibition of mycelial growth of Trichoderma viride with extracts of pearl millet. We have identified several proteins with antifungal properties in the seeds of pearl millet. One of these proteins has been purified to homogeneity and characterized. The purified protein has a molecular mass of 25 kDa. The N-terminal sequence of the protein (25 residues) shows homology to non-specific lipid transfer proteins (LTPs) of cotton, wheat and barley. The purified LTP inhibited mycelial growth of T. viride and the rice sheath blight fungus, Rhizoctonia solani in vitro.

Keywords: pathogenesis-related protein; Pennisetum glaucum; Rhizoctonia solani; Trichoderma viride
Subjects: antifungal protein from pearl millet seeds; pathogenesis-related protein, defense-related protein; Pennisetum glaucum; Rhizoctonia solani; Trichoderma viride

Published: September 1, 2001  Show citation

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Velazhahan, R., Radhajeyalakshmi, R., Thangavelu, R., & Muthukrishnan, S. (2001). An Antifungal Protein Purified from Pearl Millet Seeds Shows Sequence Homology to Lipid Transfer Proteins. Biologia plantarum, 44(3), 417-421. doi: 10.1023/A:1012463315579
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