biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:621-624, 2005 | DOI: 10.1007/s10535-005-0061-2

Protein analysis of dwarfed transgenic rice plants overexpressing GA2-oxidase gene

M. Hajduch1,2,*, H. Tanaka1, Y. Morinaka1, Y. Otake1, H. Nakamura1, T. Kayano1, Y. Koga-Ban1
1 Plant Biotechnology Department, National Institute of Agrobiological Sciences, Ibaraki, Japan
2 Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra, Slovak Republic

Using 2-D electrophoresis, we analyzed proteins from transgenic rice overexpressing gibberellin acid (GA) catabolic enzyme, GA2-oxidase. These results indicate eight specific proteins differentially expressed in the transformed rice stems of T1 generation, but non in case of T2 generation. Proteins isolated from different stages of leaves of T1 generation showed no significant differences, except one-month-old leaf, where five differentially expressed proteins are visible.

Keywords: dwarf plants; gene silencing; Oryza sativa; protein analysis; 2-D electrophoresis
Subjects: dwarf plants; Oryza sativa; GA2-oxidase; protein, analysis, transgenic rice; rice, dwarf transgenic plants; transgenic plants

Received: January 8, 2004; Accepted: November 30, 2004; Published: December 1, 2005  Show citation

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Hajduch, M., Tanaka, H., Morinaka, Y., Otake, Y., Nakamura, H., Kayano, T., & Koga-Ban, Y. (2005). Protein analysis of dwarfed transgenic rice plants overexpressing GA2-oxidase gene. Biologia plantarum, 49(4), 621-624. doi: 10.1007/s10535-005-0061-2
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