biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:121-125, 2008 | DOI: 10.1007/s10535-008-0024-5

Improvement of in vitro androgenesis in niger using amino acids and polyamines

B. P. Hema1, H. N. Murthy1,*
1 Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad, India

The effects of amino acids (arginine, aspargine, cysteine, glutamine, glycine and proline) and polyamines (putrescine and spermidine) on embryogenesis and plant regeneration from cultured anthers of Guizotia abyssinica (L. f.) Cass. cv. Ootacamund was studied. Supplementation of amino acids (0.5-5.0 mM) to the induction medium individually and in combination, improved embryo yield. B5 medium supplemented with 2 mM proline, 10 µM 2,4-dichlorophenoxyacetic acid, 2 µM kinetin and 0.2 M sucrose induced highest number of embryos (63 per 60 anthers cultured). Addition of polyamines (5-200 µM) to the same medium also enhanced the rate of embryogenesis.

Keywords: anther culture; embryogenesis; Guizotia abyssinica
Subjects: abscisic acid (ABA); amino acids; androgenesis; anther culture; cytokinins; gibberellic acid, gibberellins; Guizotia abyssinica; in vitro culture, somatic embryogenesis; nutrient medium, Gamborg (B5)

Received: May 29, 2006; Accepted: December 1, 2006; Published: March 1, 2008  Show citation

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Hema, B.P., & Murthy, H.N. (2008). Improvement of in vitro androgenesis in niger using amino acids and polyamines. Biologia plantarum, 52(1), 121-125. doi: 10.1007/s10535-008-0024-5
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