biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:135-140, 2010 | DOI: 10.1007/s10535-010-0021-3

Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers

B. Sikdar1, M. Bhattacharya2, A. Mukherjee3, A. Banerjee2, E. Ghosh2, B. Ghosh2, S. C. Roy2,*
1 Department of Genetic Engineering and Biotechnology, University of Rajsahi, Rajsahi, Bangladesh
2 Department of Botany, University of Calcutta, Kolkata, India
3 Department of Botany, Dinabandhu Mahavidyalaya, Bongaon, India

Biochemical and molecular markers have been used on eleven species of Cucurbitaceae collected from lower Gangetic plains. Six enzyme systems were selected. Among 40 primers examined, 14 random amplified polymorphic DNA (RAPD) and 10 inter-simple sequence repeat (ISSR) primers were selected for the analysis. Generated RAPD (100) and ISSR (100) fragments showed high variations among the species. Jaccard similarity coefficients were used for the evaluation of pairwise genetic divergence; cluster analysis of the similarity matrices was performed to estimate interspecific diversity. Further, principal coordinate analysis was performed to evaluate the resolving power of the three marker systems to differenciate among the species.

Keywords: cluster analysis; Jaccard similarity coefficient; molecular marker; primer
Subjects: cucumber; Cucumis sativus; Cucurbita maxima; Cucurbitaceae members; genetic diversity; inter-simple sequence repeat (ISSR); random amplified polymorphic DNA (RAPD); UPGMA dengrogram

Received: April 5, 2008; Accepted: September 15, 2008; Published: March 1, 2010  Show citation

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Sikdar, B., Bhattacharya, M., Mukherjee, A., Banerjee, A., Ghosh, E., Ghosh, B., & Roy, S.C. (2010). Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers. Biologia plantarum, 54(1), 135-140. doi: 10.1007/s10535-010-0021-3
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