biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 67:105-113, 2023 | DOI: 10.32615/bp.2023.010

Self-interaction of Tomato spotted wilt virus NSs protein enhances gene silencing suppressor activity, but is dispensable as avirulence determinant on pepper

A. ALMÁSI1, *, K. NEMES2, R. SÁRAY1, Á. GELLÉRT3, N. INCZE4, P. VÁGI5, E. BADICS4, V. SOÓS4, K. SALÁNKI1
1 Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, H-1022 Budapest, Hungary
2 European Union Reference Laboratory for Foodborne Viruses, Swedish Food Agency, SE-75237 Uppsala, Sweden
3 Institute for Veterinary Medical Research, Eötvös Loránd Research Network, H-1143 Budapest, Hungary
4 Agricultural Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, H-2462 Martonvásár, Hungary
5 Institute of Experimental Medicine, Eötvös Loránd Research Network, H-1083 Budapest, Hungary

Tomato spotted wilt virus (TSWV) has significant economic impact on horticulture worldwide. One of the five proteins encoded by TSWV genome is the multifunctional NSs protein, which is a viral suppressor of RNA silencing (VSR) besides functioning as the effector of Tsw resistance gene in resistant pepper cultivars. In this study we demonstrate the in vivo self-interaction of NSs protein using bimolecular fluorescence complementation and yeast two-hybrid assays, and propose that a highly charged alpha helix located at the second half of the protein is required for self-interaction. Furthermore, we confirmed that self-interaction is not required for its effector function on pepper. Moreover, self-interaction is dispensable for gene silencing suppressor activity, although it enhances the suppression efficiency.

Keywords: avirulence determinant, hypersensitive reaction, NSs protein, self-interaction, Tomato spotted wilt virus, viral suppressor of RNA silencing.

Received: December 31, 2022; Revised: April 3, 2023; Accepted: April 6, 2023; Published online: May 31, 2023  Show citation

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ALMÁSI, A., NEMES, K., SÁRAY, R., GELLÉRT, Á., INCZE, N., VÁGI, P., ... SALÁNKI, K. (2023). Self-interaction of Tomato spotted wilt virus NSs protein enhances gene silencing suppressor activity, but is dispensable as avirulence determinant on pepper. Biologia plantarum, 67, Article 105-113. https://doi.org/10.32615/bp.2023.010
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